Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,926,888 C→A 8.9% G651V (GGC→GTC)  ptrB ← protease II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,926,8880CA 8.9% 274.9 / 14.1 79G651V (GGC→GTC) ptrBprotease II
Reads supporting (aligned to +/- strand):  ref base C (36/36);  new base A (4/3);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

GTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGG  >  NC_000913/1926748‑1927035
                                                                                                                                            |                                                                                                                                                   
gTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTcc                                                                                                                                             <  2:1073713/149‑1 (MQ=255)
         tGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCAt                                                                                                                                           <  2:690560/142‑1 (MQ=255)
         tGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCAt                                                                                                                                           >  1:690560/1‑142 (MQ=255)
         tGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa                                                                                                                                    >  4:698236/1‑149 (MQ=255)
                   tCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATa                                                                                                                          >  2:1142403/1‑149 (MQ=255)
                        aaaaTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                                                                            >  2:680803/1‑126 (MQ=255)
                        aaaaTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                                                                            <  1:680803/126‑1 (MQ=255)
                         aaaTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGa                                                                                                                                                 >  3:299642/1‑120 (MQ=255)
                         aaaTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGa                                                                                                                                                 <  4:299642/120‑1 (MQ=255)
                               ttAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa                                                                                                                                    >  2:582064/1‑127 (MQ=255)
                               ttAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa                                                                                                                                    <  1:582064/127‑1 (MQ=255)
                               ttAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAt                                                                                                              <  3:698236/149‑1 (MQ=255)
                                    ccGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGtt                                                                                                         <  1:1142403/149‑1 (MQ=255)
                                          gCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGc                                                                                                   >  4:1022279/1‑149 (MQ=255)
                                                cGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATg                                                                                                                   <  1:523126/127‑1 (MQ=255)
                                                cGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTCCGTACGATTGAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATg                                                                                                                   >  2:523126/1‑127 (MQ=255)
                                                    tAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAAtt                                                                                         <  3:1022279/149‑1 (MQ=255)
                                                    tAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATCTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCAt                                                                                                                                           >  2:782105/1‑99 (MQ=255)
                                                    tAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATCTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCAt                                                                                                                                           <  1:782105/99‑1 (MQ=255)
                                                         ttCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCg                                                                                    >  3:497611/1‑149 (MQ=255)
                                                            ccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa                                                                                                                                    <  1:210008/98‑1 (MQ=255)
                                                            ccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa                                                                                                                                    >  2:210008/1‑98 (MQ=255)
                                                                     gcgcGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGcc                                                                        <  4:497611/149‑1 (MQ=255)
                                                                         gACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGtt                                                                    >  1:30459/1‑149 (MQ=255)
                                                                            cAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTccc                                                                 >  3:1145199/1‑149 (MQ=255)
                                                                                aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGc                                                                                     >  1:1069200/1‑125 (MQ=255)
                                                                                aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGc                                                                                     <  2:1069200/125‑1 (MQ=255)
                                                                                 aaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGcc                                                                        >  1:595230/1‑137 (MQ=255)
                                                                                 aaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGcc                                                                        <  2:595230/137‑1 (MQ=255)
                                                                                 aaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTccc                                                                 <  3:1330462/144‑1 (MQ=255)
                                                                                 aaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTccc                                                                 >  4:1330462/1‑144 (MQ=255)
                                                                                   gCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCg                                                                                    >  4:153084/1‑123 (MQ=255)
                                                                                   gCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCg                                                                                    <  3:153084/123‑1 (MQ=255)
                                                                                    cATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATc                                                                                                             >  3:497758/1‑97 (MQ=255)
                                                                                    cATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATc                                                                                                             <  4:497758/97‑1 (MQ=255)
                                                                                    cATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGc                                                         >  2:1465600/1‑149 (MQ=255)
                                                                                     atatTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCa                                                        >  4:1357141/1‑149 (MQ=255)
                                                                                     atatTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCa                                                        <  2:30459/149‑1 (MQ=255)
                                                                                        ttCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATaaaa                                                                                                                       >  2:207446/1‑83 (MQ=255)
                                                                                        ttCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATaaaa                                                                                                                       <  1:207446/83‑1 (MQ=255)
                                                                                        ttCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCt                                                     >  2:1254568/1‑149 (MQ=255)
                                                                                          ccATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTga                                                   >  1:515493/1‑149 (MQ=255)
                                                                                            aTCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGcc                                                                        <  1:1127129/126‑1 (MQ=255)
                                                                                            aTCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGcc                                                                        >  2:1127129/1‑126 (MQ=255)
                                                                                               gCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATc                                              <  3:1357141/149‑1 (MQ=255)
                                                                                                 tACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGt                                            <  1:1465600/149‑1 (MQ=255)
                                                                                                 tACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGt                                            <  2:515493/149‑1 (MQ=255)
                                                                                                    gCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCa                                         >  1:1399175/1‑149 (MQ=255)
                                                                                                      cTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTa                                                                                       >  1:205645/1‑101 (MQ=255)
                                                                                                      cTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTa                                                                                       <  2:205645/101‑1 (MQ=255)
                                                                                                       ttCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAAc                                      >  2:954428/1‑149 (MQ=255)
                                                                                                           tACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATg                                                                                                                   <  1:1287444/68‑1 (MQ=255)
                                                                                                           tACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATg                                                                                                                   >  2:1287444/1‑68 (MQ=255)
                                                                                                            aCGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTc                                 <  4:1145199/149‑1 (MQ=255)
                                                                                                               aTTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACa                                                                                                                                  <  4:578699/49‑1 (MQ=255)
                                                                                                               aTTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACa                                                                                                                                  >  3:578699/1‑49 (MQ=255)
                                                                                                                 ttAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTAc                            <  2:1399175/149‑1 (MQ=255)
                                                                                                                         aCCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTAc                            >  1:672025/1‑141 (MQ=255)
                                                                                                                         aCCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTAc                            <  2:672025/141‑1 (MQ=255)
                                                                                                                          ccAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTga                                                   >  4:805551/1‑117 (MQ=255)
                                                                                                                          ccAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTga                                                   <  3:805551/117‑1 (MQ=255)
                                                                                                                             tctgtACCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCgg                >  1:963461/5‑149 (MQ=255)
                                                                                                                              aTTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGa               <  1:954428/149‑1 (MQ=255)
                                                                                                                                    cgacATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGcc         >  1:1226621/4‑149 (MQ=255)
                                                                                                                                     gacATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTga                                                   >  4:1035007/3‑106 (MQ=255)
                                                                                                                                     gacATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTga                                                   <  3:1035007/104‑1 (MQ=255)
                                                                                                                                     gacATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTg                                           <  4:1001573/112‑1 (MQ=255)
                                                                                                                                     gacATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTg                                           >  3:1001573/3‑114 (MQ=255)
                                                                                                                                      ccATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCaaa                                       <  2:1154736/117‑1 (MQ=255)
                                                                                                                                      ccATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCaaa                                       >  1:1154736/1‑117 (MQ=255)
                                                                                                                                      ccATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCa                          >  3:385673/1‑130 (MQ=255)
                                                                                                                                      ccATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCa                          <  4:385673/130‑1 (MQ=255)
                                                                                                                                       cATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCtgt      <  2:963461/149‑1 (MQ=255)
                                                                                                                                          ggCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTTCACCTGAGAATCGt                                            >  3:945404/1‑108 (MQ=255)
                                                                                                                                          ggCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTTCACCTGAGAATCGt                                            <  4:945404/108‑1 (MQ=255)
                                                                                                                                          ggCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCg   <  1:1254568/149‑1 (MQ=255)
                                                                                                                                           gCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTc                                 >  4:1156922/1‑118 (MQ=255)
                                                                                                                                           gCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCgg  >  3:629923/1‑149 (MQ=255)
                                                                                                                                           gCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGCGCAAACCGGTc                                 <  3:1156922/118‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
GTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGG  >  NC_000913/1926748‑1927035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: