| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,987,857 | C→T | 7.8% | intergenic (‑99/+16) | yecJ ← / ← azuC | DUF2766 family protein/acid‑inducible small membrane‑associated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,987,857 | 0 | C | T | 7.8% | 165.6 / 2.9 | 51 | intergenic (‑99/+16) | yecJ/azuC | DUF2766 family protein/acid‑inducible small membrane‑associated protein |
| Reads supporting (aligned to +/- strand): ref base C (23/24); new base T (2/2); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATT > NC_000913/1987713‑1987995 | ccACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAg > 4:372635/1‑149 (MQ=255) cAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑tt < 3:372635/149‑1 (MQ=255) ttCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATc < 4:843362/149‑1 (MQ=255) ttggCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCTCCGGCAGG‑ttt < 1:1072056/130‑1 (MQ=255) ttggCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCTCCGGCAGG‑ttt > 2:1072056/3‑132 (MQ=255) cAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGc > 3:1072130/1‑119 (MQ=255) cAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGc < 4:1072130/119‑1 (MQ=255) aGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTgg > 3:1082144/1‑149 (MQ=255) gCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTg > 3:1453885/1‑143 (MQ=255) gCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTg < 4:1453885/143‑1 (MQ=255) cATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCtt < 4:1082144/149‑1 (MQ=255) aTAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc > 4:924672/1‑139 (MQ=255) aTAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTAGCGGAACATATTGCCTGGCGGTACGTCTTTGAAc < 3:924672/139‑1 (MQ=255) ttttGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑tttttt < 1:81011/95‑1 (MQ=255) ttttGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑tttttt > 2:81011/1‑95 (MQ=255) gTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTg > 3:153750/1‑85 (MQ=255) gTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTg < 4:153750/85‑1 (MQ=255) gTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGt > 2:549763/1‑110 (MQ=255) gTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGt < 1:549763/110‑1 (MQ=255) ttATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCg < 2:427060/90‑1 (MQ=255) ttATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCg > 1:427060/1‑90 (MQ=255) acaTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCaa < 4:762737/114‑1 (MQ=255) acaTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCaa > 3:762737/1‑114 (MQ=255) gCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGcc < 3:1331476/77‑1 (MQ=255) gCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGcc > 4:1331476/1‑77 (MQ=255) cGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAg < 4:1142504/107‑1 (MQ=255) cGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAg > 3:1142504/1‑107 (MQ=255) tCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATc < 2:566922/57‑1 (MQ=255) tCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATc > 1:566922/1‑57 (MQ=255) tCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTAc > 4:1281544/1‑79 (MQ=255) tCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTAc < 3:1281544/79‑1 (MQ=255) acaTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATa > 2:935423/1‑98 (MQ=255) acaTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATa < 1:935423/98‑1 (MQ=255) atcaGTACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCttt < 1:1384186/71‑1 (MQ=255) atcaGTACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCttt > 2:1384186/1‑71 (MQ=255) atcaGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGt > 1:170363/1‑67 (MQ=255) atcaGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGt < 2:170363/67‑1 (MQ=255) tcaGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtttt < 3:142179/112‑1 (MQ=255) tcaGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtttt > 4:142179/1‑112 (MQ=255) aGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTg > 3:1053696/1‑78 (MQ=255) aGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTg < 4:1053696/78‑1 (MQ=255) acaAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATa > 4:93580/1‑78 (MQ=255) acaAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATa < 3:93580/78‑1 (MQ=255) aaaaaaaCCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTa > 1:256935/1‑149 (MQ=255) aaaaaaCCTGCTCCGGCAGGTTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGaa < 3:323950/64‑1 (MQ=18) aaaaaaCCTGCTCCGGCAGGTTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTGTGaa > 4:323950/1‑64 (MQ=17) aaaaaCCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAtt < 2:256935/149‑1 (MQ=255) aaCCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTc < 2:1334553/90‑1 (MQ=255) aaCCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTc > 1:1334553/1‑90 (MQ=255) tGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtt > 3:471477/1‑92 (MQ=255) tGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtt < 4:471477/92‑1 (MQ=255) | CCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATT > NC_000913/1987713‑1987995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |