Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,044,725 T→A 9.0% intergenic (+101/‑213) asnT → / → yeeJ tRNA‑Asn/putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,044,7250TA 9.0% 225.1 / 11.1 67intergenic (+101/‑213)asnT/yeeJtRNA‑Asn/putative adhesin
Reads supporting (aligned to +/- strand):  ref base T (28/33);  new base A (2/4);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

GGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGT  >  NC_000913/2044577‑2044873
                                                                                                                                                    |                                                                                                                                                    
ggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCt                                                                                                                                                      <  3:1307892/149‑1 (MQ=255)
     gTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATg                                                                                                                                                 <  3:1173323/149‑1 (MQ=255)
     gTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATg                                                                                                                                                 <  2:1031929/149‑1 (MQ=255)
      ttAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATgg                                                                                                                                                >  4:1067447/1‑149 (MQ=255)
      ttAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATgg                                                                                                                                                <  2:1406430/149‑1 (MQ=255)
        aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                              <  1:420837/149‑1 (MQ=255)
           gcaatatgattgaaaattaataacttttcgttaggcagttttgggtgtgagttgcaagaggggagactactgaataactcaagttttataatcgaggggaaaatggtgatggcgttcatagcaaaaCGCCCTCGACCTTTATGGTTGAg                                                                                                                                           <  4:825130‑M1/23‑1 (MQ=255)
            cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAgg                                                                                                                                          <  3:676340/149‑1 (MQ=38)
            cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAgg                                                                                                                                          <  1:120014/149‑1 (MQ=38)
             aatatgattgaaaattaataacttttcgttaggcagttttgggtgtgagttgcaagaggggagactactgaataactcaagttttataatcgaggggaaaatggtgatggcgttcatagcaaaaCGCCCTCGACCTTTATGGTTGAggg                                                                                                                                         >  3:825130‑M1/125‑149 (MQ=255)
                 gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                              <  4:1421357/140‑1 (MQ=255)
                 gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                              >  3:1421357/1‑140 (MQ=255)
                        gTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAgg                                                                                                                                          <  1:1021692/137‑1 (MQ=255)
                         ttCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAgg                                                                                                                                          >  2:1098257/1‑136 (MQ=255)
                               tCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATg                                                                                                                                                 >  4:946889/1‑123 (MQ=255)
                               tCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATg                                                                                                                                                 <  3:946889/123‑1 (MQ=255)
                                aacttttcgttaggcagttttgggtgtgagttgcaagaggggagactactgaataactcaagttttataatcgaggggaaaatggtgatggcgttcatagcaaaaCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                          >  4:1305520‑M1/106‑129 (MQ=255)
                                aacttttcgttaggcagttttgggtgtgagttgcaagaggggagactactgaataactcaagttttataatcgaggggaaaatggtgatggcgttcatagcaaaaCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                          <  3:1305520‑M1/24‑1 (MQ=255)
                                         ggagCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAATATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTAt                                                                                                                                                  <  1:959246/112‑1 (MQ=255)
                                         ggagCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTAt                                                                                                                                                  >  2:959246/1‑112 (MQ=255)
                                                  ttCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAt                                                                                                                              >  3:19382/1‑123 (MQ=255)
                                                  ttCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAt                                                                                                                              <  4:19382/123‑1 (MQ=255)
                                                     tAAAAATTCGCTTTTTTAGCGCAATGTCACTGACGTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGCTTTGCTATGAACGCCATCACCATTTTCCCCTCGATta                                                                                                 <  1:1195547/149‑1 (MQ=255)
                                                     tAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATg                                                                                                                                                 <  1:108785/101‑1 (MQ=255)
                                                     tAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATg                                                                                                                                                 >  2:108785/1‑101 (MQ=255)
                                                                ttttttAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATaaaa                                                                                            <  4:1327992/143‑1 (MQ=255)
                                                                ttttttAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATaaaa                                                                                            >  3:1327992/1‑143 (MQ=255)
                                                                 caagaggggagactactgaataactcaagttttataatcgaggggaaaatggtgatggcgttcatagcaaaaCGCCCTCGACCTTTATGGTTGAg                                                                                                                                           <  1:599975‑M1/23‑1 (MQ=255)
                                                                 caagaggggagactactgaataactcaagttttataatcgaggggaaaatggtgatggcgttcatagcaaaaCGCCCTCGACCTTTATGGTTGAg                                                                                                                                           >  2:599975‑M1/73‑95 (MQ=255)
                                                                     tAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTAtt                                                                                 <  3:1067447/149‑1 (MQ=255)
                                                                              gTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGtct                                                                        >  4:591327/1‑149 (MQ=255)
                                                                                 tgaataactcaagttttataatcgaggggaaaatggtgatggcgttcatagcaaaaCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                          >  2:1376877‑M1/57‑80 (MQ=255)
                                                                                 tgaataactcaagttttataatcgaggggaaaatggtgatggcgttcatagcaaaaCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                          <  1:1376877‑M1/24‑1 (MQ=255)
                                                                                            ttGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAt                                                                                                                              >  4:927967/1‑81 (MQ=255)
                                                                                            ttGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAt                                                                                                                              <  3:927967/81‑1 (MQ=255)
                                                                                            ttGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACtt                                                                                         >  2:507117/1‑118 (MQ=255)
                                                                                            ttGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACtt                                                                                         <  1:507117/118‑1 (MQ=255)
                                                                                            ttGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACt                                                          >  1:1138737/1‑149 (MQ=255)
                                                                                              gAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTg                                                                                                                                  >  2:381992/1‑75 (MQ=255)
                                                                                              gAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTg                                                                                                                                  <  1:381992/75‑1 (MQ=255)
                                                                                              gAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCGTTTTCCCCTCGATTATAAAACTTGa                                                                                       <  3:1441106/118‑1 (MQ=255)
                                                                                              gAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCGTTTTCCCCTCGATTATAAAACTTGa                                                                                       >  4:1441106/1‑118 (MQ=255)
                                                                                                  aTTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACAcc                                                    >  2:26476/1‑149 (MQ=255)
                                                                                                   ttGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccc                                                                                                         >  4:808484/1‑95 (MQ=255)
                                                                                                   ttGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccc                                                                                                         <  3:808484/95‑1 (MQ=255)
                                                                                                    aatcgaggggaaaatggtgatggcgttcatagcaaaaCGCCCTCGACCTTTATGGTTGAg                                                                                                                                           <  4:1444400‑M1/23‑1 (MQ=255)
                                                                                                    aatcgaggggaaaatggtgatggcgttcatagcaaaaCGCCCTCGACCTTTATGGTTGAg                                                                                                                                           >  3:1444400‑M1/38‑60 (MQ=255)
                                                                                                           tAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc                                           <  1:26476/149‑1 (MQ=255)
                                                                                                           tAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc                                           <  3:591327/149‑1 (MQ=255)
                                                                                                           tAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc                                           <  2:1138737/149‑1 (MQ=255)
                                                                                                                 aTAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCagta                                                                            >  3:332247/1‑110 (MQ=255)
                                                                                                                 aTAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCagta                                                                            <  4:332247/110‑1 (MQ=255)
                                                                                                                       ggTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGt                               >  3:1346862/1‑149 (MQ=255)
                                                                                                                        gTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTcccc                                                                                                        <  3:1426811/75‑1 (MQ=255)
                                                                                                                        gTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTcccc                                                                                                        >  4:1426811/1‑75 (MQ=255)
                                                                                                                           tttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttatt                           <  4:1346862/149‑1 (MQ=255)
                                                                                                                              aaCATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAAt                        >  2:1153232/1‑149 (MQ=255)
                                                                                                                               aCATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAAc                                      <  2:1344736/134‑1 (MQ=255)
                                                                                                                               aCATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAAc                                      >  1:1344736/1‑134 (MQ=255)
                                                                                                                                   cTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGaaaa                                 <  2:1137560/135‑1 (MQ=255)
                                                                                                                                   cTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGaaaa                                 >  1:1137560/1‑135 (MQ=255)
                                                                                                                                       aaCGCCCTCAACCATAAAGGTCGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTAtt                                                                                 <  3:908834‑M1/81‑1 (MQ=255)
                                                                                                                                       aaCGCCCTCAACCATAAAGGTCGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTAtt                                                                                 >  4:908834‑M1/3‑83 (MQ=255)
                                                                                                                                            CCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTg          <  3:713377‑M1/149‑1 (MQ=255)
                                                                                                                                             CTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAAttt                      >  4:231614‑M1/1‑136 (MQ=255)
                                                                                                                                             CTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAAttt                      <  3:231614‑M1/136‑1 (MQ=255)
                                                                                                                                                gACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTaa                         >  1:1089476/1‑130 (MQ=255)
                                                                                                                                                gACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTaa                         <  2:1089476/130‑1 (MQ=255)
                                                                                                                                                    tttATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGt  >  1:1010836/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGT  >  NC_000913/2044577‑2044873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: