Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 173,573 T→G 6.3% intergenic (+129/+29) fhuB → / ← hemL iron(3+)‑hydroxamate import ABC transporter permease/glutamate‑1‑semialdehyde aminotransferase (aminomutase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913173,5730TG 6.3% 285.4 / 9.5 80intergenic (+129/+29)fhuB/hemLiron(3+)‑hydroxamate import ABC transporter permease/glutamate‑1‑semialdehyde aminotransferase (aminomutase)
Reads supporting (aligned to +/- strand):  ref base T (43/32);  new base G (2/3);  total (45/35)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.83e-01

GAGCCGTTAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCG  >  NC_000913/173435‑173719
                                                                                                                                          |                                                                                                                                                  
gagCCGTTAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATGCGGCGTGAAcgccttat                                                                                                                                          <  2:502932/149‑5 (MQ=255)
     gTTAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGAc                                                                                                                                     >  2:248172/1‑149 (MQ=255)
                        ggCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCAt                                                                                                                                          >  2:78545/1‑125 (MQ=255)
                        ggCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCAt                                                                                                                                          <  1:78545/125‑1 (MQ=255)
                         gCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGa                                                                                                                        >  2:374000/1‑142 (MQ=255)
                         gCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGa                                                                                                                        <  1:374000/142‑1 (MQ=255)
                                    cTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAacac                                                                                                      >  1:1330823/1‑149 (MQ=255)
                                               aaGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTc                                                                                                                                                   >  2:894098/1‑93 (MQ=255)
                                               aaGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTc                                                                                                                                                   <  1:894098/93‑1 (MQ=255)
                                               aaGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGAt                                                                                                                       <  4:423325/121‑1 (MQ=255)
                                               aaGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGAt                                                                                                                       >  3:423325/1‑121 (MQ=255)
                                                   gTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAAc                                                                                                                                 <  2:112024/107‑1 (MQ=255)
                                                   gTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAAc                                                                                                                                 >  1:112024/1‑107 (MQ=255)
                                                     ttACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGa                                                                                     <  2:1330823/149‑1 (MQ=255)
                                                         gcgACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAac                                                                                                        <  2:1443944/126‑1 (MQ=255)
                                                         gcgACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAac                                                                                                        >  1:1443944/1‑126 (MQ=255)
                                                         gcgACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGgt                                                                                 >  3:235743/1‑149 (MQ=255)
                                                           gACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATGCGGCGTGAAcgccttat                                                                                                                                          >  3:1243600/1‑86 (MQ=25)
                                                           gACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATGCGGCGTGAAcgccttat                                                                                                                                          <  4:1243600/90‑5 (MQ=25)
                                                           gACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGgtgt                                                                               <  4:235743/149‑1 (MQ=255)
                                                              aaCGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATcc                                                                                                                                        <  2:555893/89‑1 (MQ=255)
                                                              aaCGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATcc                                                                                                                                        >  1:555893/1‑89 (MQ=255)
                                                              aaCGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTAt                                                                            <  1:248172/149‑1 (MQ=255)
                                                               aCGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTAtt                                                                           <  1:1376121/149‑1 (MQ=255)
                                                                         gATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTAt                                                                            <  1:71095/138‑1 (MQ=255)
                                                                         gATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTAt                                                                            >  2:71095/1‑138 (MQ=255)
                                                                                 gCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCtgtg                                                         >  4:1440860/1‑149 (MQ=255)
                                                                                            ttATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGAt                                                                                    >  4:1322239/1‑111 (MQ=255)
                                                                                            ttATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGAt                                                                                    <  3:1322239/111‑1 (MQ=255)
                                                                                                 gggCCTACAAACGGCCCCGAATCGTAGGTCGGATATGGCGTTCACGCCGCATCCGACaaa                                                                                                                                  >  2:102170/1‑60 (MQ=255)
                                                                                                 gggCCTACAAACGGCCCCGAATCGTAGGTCGGATATGGCGTTCACGCCGCATCCGACaaa                                                                                                                                  <  1:102170/60‑1 (MQ=255)
                                                                                                 gggCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGAc                                               >  3:207765/1‑143 (MQ=255)
                                                                                                 gggCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGAc                                               <  4:207765/143‑1 (MQ=255)
                                                                                                  ggCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGTCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCt                                                            <  1:422827/129‑1 (MQ=255)
                                                                                                  ggCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCt                                                            >  2:422827/1‑129 (MQ=255)
                                                                                                  ggCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACATGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGAc                                               <  4:935187/142‑1 (MQ=255)
                                                                                                  ggCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACATGCAGCATCGATGGGGTTATTGATATCTTCCATGCTGTGCGCCACGGAc                                               >  3:935187/1‑142 (MQ=255)
                                                                                                      tACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACAccc                                                                                                    <  3:742503/85‑1 (MQ=255)
                                                                                                      tACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACAccc                                                                                                    >  4:742503/1‑85 (MQ=255)
                                                                                                      tACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATg                                                                                   <  4:1207945/102‑1 (MQ=255)
                                                                                                      tACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATg                                                                                   <  2:915594/102‑1 (MQ=255)
                                                                                                      tACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATg                                                                                   >  1:915594/1‑102 (MQ=255)
                                                                                                      tACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATg                                                                                   >  3:1207945/1‑102 (MQ=255)
                                                                                                      tACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGa                                                <  2:50895/137‑1 (MQ=255)
                                                                                                      tACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGa                                                >  1:50895/1‑137 (MQ=255)
                                                                                                      tACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAg                                         >  1:1097266/1‑144 (MQ=255)
                                                                                                      tACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAg                                         <  2:1097266/144‑1 (MQ=255)
                                                                                                         aaaCGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTcc                                                                 >  1:1052715/1‑117 (MQ=255)
                                                                                                         aaaCGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTcc                                                                 <  2:1052715/117‑1 (MQ=255)
                                                                                                         aaaCGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTc                                 >  4:851625/1‑149 (MQ=255)
                                                                                                             ggCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAAc                             <  3:851625/149‑1 (MQ=255)
                                                                                                                 ccGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTg                         >  3:294065/1‑149 (MQ=255)
                                                                                                                 ccGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTg                         >  2:186106/1‑149 (MQ=255)
                                                                                                                 ccGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTg                         >  2:333262/1‑149 (MQ=255)
                                                                                                                 ccGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTg                         >  2:72440/1‑149 (MQ=255)
                                                                                                                  cGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGa                        >  4:566005/1‑149 (MQ=255)
                                                                                                                   gAATNGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTc                                                                  >  1:1451773/1‑106 (MQ=255)
                                                                                                                   gAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTc                                                                  <  2:1451773/106‑1 (MQ=255)
                                                                                                                         tttgTCGGATGCGGCGTGAACGCCTTATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATaaa                                          >  3:1136197/4‑124 (MQ=38)
                                                                                                                         tttgTCGGATGCGGCGTGAACGCCTTATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATaaa                                          <  4:1136197/121‑1 (MQ=38)
                                                                                                                           ggTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAg               >  2:1376493/1‑149 (MQ=255)
                                                                                                                            gTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTgc                                                                                             >  2:824020/1‑70 (MQ=255)
                                                                                                                            gTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTgc                                                                                             <  1:824020/70‑1 (MQ=255)
                                                                                                                            gTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAgg              >  4:498207/1‑149 (MQ=255)
                                                                                                                             tCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGt             <  3:1440860/149‑1 (MQ=255)
                                                                                                                                gATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTaaa          >  1:86406/1‑149 (MQ=255)
                                                                                                                                gATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTaaa          >  4:974814/1‑149 (MQ=255)
                                                                                                                                 aTAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGc                           <  4:1035579/131‑1 (MQ=255)
                                                                                                                                 aTAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGc                           >  3:1035579/1‑131 (MQ=255)
                                                                                                                                 aTAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAg               <  4:1176277/143‑1 (MQ=255)
                                                                                                                                 aTAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAg               >  3:1176277/1‑143 (MQ=255)
                                                                                                                                   aaGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAacac       >  1:729382/1‑149 (MQ=255)
                                                                                                                                    aGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGt             <  1:861041/142‑1 (MQ=255)
                                                                                                                                    aGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGt             >  2:861041/1‑142 (MQ=255)
                                                                                                                                      gCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATc                                                                                       <  1:8235/66‑1 (MQ=255)
                                                                                                                                      gCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATc                                                                                       >  2:8235/1‑66 (MQ=255)
                                                                                                                                      gCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCa                                                                <  2:1089534/89‑1 (MQ=255)
                                                                                                                                      gCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCa                                                                >  1:1089534/1‑89 (MQ=255)
                                                                                                                                        gTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCg  >  1:723070/1‑149 (MQ=255)
                                                                                                                                        gTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCACGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCg  >  3:952035/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
GAGCCGTTAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCG  >  NC_000913/173435‑173719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: