Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,145,313 C→A 100% S683Y (TCC→TAC)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,145,3130CA100.0% 560.6 / NA 143S683Y (TCC→TAC) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (66/77);  total (66/77)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 143 total aligned reads displayed.

TGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGC  >  NC_000913/2145171‑2145455
                                                                                                                                              |                                                                                                                                              
tGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatga                                                                                                                                          <  1:552128/149‑1 (MQ=255)
tGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatga                                                                                                                                          <  4:1233655/149‑1 (MQ=255)
 gTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatgat                                                                                                                                         <  4:632422/149‑1 (MQ=255)
    ggAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCa                                                                                                                                      >  3:1094357/1‑149 (MQ=255)
     gAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCAc                                                                                                                                     >  3:548874/1‑149 (MQ=255)
     gAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCAc                                                                                                                                     <  4:1148083/149‑1 (MQ=255)
        cggcggCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGa                                                                                                                                  <  1:565058/149‑1 (MQ=255)
        cggcggCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGa                                                                                                                                  <  3:47405/149‑1 (MQ=255)
        cggcggCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGa                                                                                                                                  <  4:1218445/149‑1 (MQ=255)
         ggcggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGAc                                                                                                                                 <  4:1094357/149‑1 (MQ=255)
              cAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCAtc                                                                                                                            <  4:1026644/149‑1 (MQ=255)
                 ttACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGAc                                                                                                                                 >  1:218842/1‑141 (MQ=255)
                  tACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGc                                                                                                                        <  4:1213226/149‑1 (MQ=255)
                  tACGAAGTGCATTACAGTATTACGCCGGTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGc                                                                                                                        >  4:1234003/1‑149 (MQ=255)
                      acgTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAAc                                                                                                                    >  1:459068/1‑149 (MQ=255)
                        gTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACcg                                                                                                                  >  1:70299/1‑149 (MQ=255)
                                cAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCa                                                                                                                                      >  1:917400/1‑121 (MQ=255)
                                cAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCa                                                                                                                                      <  2:917400/121‑1 (MQ=255)
                                cAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGtt                                                                                                          >  2:366711/1‑149 (MQ=255)
                                  gTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGtttt                                                                                                        <  4:1145754/149‑1 (MQ=255)
                                    aTTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTga                                                                                                      <  1:366711/149‑1 (MQ=255)
                                     ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTgag                                                                                                     >  3:1121111/1‑149 (MQ=255)
                                       aCGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGaa                                                                                                   <  2:946787/149‑1 (MQ=255)
                                           cGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAaacaa                                                                                               >  1:1343686/1‑149 (MQ=255)
                                               aaGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGc                                                                                           <  4:1121111/149‑1 (MQ=255)
                                               aaGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGc                                                                                           <  3:531906/149‑1 (MQ=255)
                                               aaGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGc                                                                                           <  2:130595/149‑1 (MQ=255)
                                                  tACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTa                                                                                        >  1:1006044/1‑149 (MQ=255)
                                                   aCTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACGATGATGCACTGACGCATCTCGCCAACCGCGCCAGtttt                                                                                                        >  1:418080/1‑132 (MQ=255)
                                                   aCTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGtttt                                                                                                        <  2:418080/132‑1 (MQ=255)
                                                     tctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATcc                                                                                     >  1:937506/1‑149 (MQ=255)
                                                      ctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCct                                                                                    <  1:779806/149‑1 (MQ=255)
                                                       tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatg                                                                                                                                           <  1:455151/93‑1 (MQ=255)
                                                       tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCctg                                                                                   <  2:70299/149‑1 (MQ=255)
                                                        ggACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCctgc                                                                                  >  1:10984/1‑149 (MQ=255)
                                                         gACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCctgct                                                                                 <  2:459068/149‑1 (MQ=255)
                                                                 caATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACgg                                                                         <  3:320373/149‑1 (MQ=255)
                                                                    tatTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGtttt                                                                                                        >  3:1212274/1‑115 (MQ=255)
                                                                    tatTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaa                                                                      >  4:725229/1‑149 (MQ=255)
                                                                      ttGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAAc                                                                    >  2:593472/1‑149 (MQ=255)
                                                                         gttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGc                                                                                                                               >  3:168259/1‑87 (MQ=255)
                                                                         gttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGt                                                                 >  1:703762/1‑149 (MQ=255)
                                                                          ttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTga                                                                                                      >  3:216273/1‑111 (MQ=255)
                                                                          ttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTga                                                                                                      >  3:636096/1‑111 (MQ=255)
                                                                          ttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTga                                                                                                      <  4:216273/111‑1 (MQ=255)
                                                                          ttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                <  3:725229/149‑1 (MQ=255)
                                                                          ttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                <  4:699469/149‑1 (MQ=255)
                                                                          ttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                <  2:937506/149‑1 (MQ=255)
                                                                          ttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                <  2:103084/149‑1 (MQ=255)
                                                                              gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTAcaca                                                            >  4:327238/1‑149 (MQ=255)
                                                                              gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTAcaca                                                            >  4:1059973/1‑149 (MQ=255)
                                                                              gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTAcaca                                                            >  3:1229767/1‑149 (MQ=255)
                                                                                      gATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                    >  2:1255089/1‑149 (MQ=255)
                                                                                        ttCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACAt                                                  >  2:1428484/1‑149 (MQ=255)
                                                                                           aGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTga                                                                                                      <  2:1034042/94‑1 (MQ=255)
                                                                                           aGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTga                                                                                                      >  1:1034042/1‑94 (MQ=255)
                                                                                           aGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc                                               >  1:259572/1‑149 (MQ=255)
                                                                                           aGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc                                               >  4:623801/1‑149 (MQ=255)
                                                                                            ggACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGccc                                              >  2:401268/1‑149 (MQ=255)
                                                                                             gACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGttt                                                                                                         <  2:208058/89‑1 (MQ=255)
                                                                                             gACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGttt                                                                                                         >  1:208058/1‑89 (MQ=255)
                                                                                                gTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCctgc                                                                                  >  3:1137882/1‑109 (MQ=255)
                                                                                                  tACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAg                                                                                                            >  4:1420243/1‑81 (MQ=255)
                                                                                                  tACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAg                                                                                                            <  3:1420243/81‑1 (MQ=255)
                                                                                                   aCCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGtt                                       <  2:259572/149‑1 (MQ=255)
                                                                                                    ccGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGttt                                      >  4:292616/1‑149 (MQ=255)
                                                                                                     cGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTa                                     >  2:1045074/1‑149 (MQ=255)
                                                                                                       aaTCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTga                                                                                                      <  2:1015592/82‑1 (MQ=255)
                                                                                                       aaTCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTga                                                                                                      >  1:1015592/1‑82 (MQ=255)
                                                                                                       aaTCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATc                                   >  2:564217/1‑149 (MQ=255)
                                                                                                        aTCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCg                                  <  3:623801/149‑1 (MQ=255)
                                                                                                        aTCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCg                                  <  3:1059973/149‑1 (MQ=255)
                                                                                                         tCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGa                                 >  2:1351169/1‑149 (MQ=255)
                                                                                                          cACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGAt                                <  4:1115056/149‑1 (MQ=255)
                                                                                                           aCGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCctgc                                                                                  <  4:1355079/98‑1 (MQ=255)
                                                                                                           aCGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCctgc                                                                                  >  3:1355079/1‑98 (MQ=255)
                                                                                                            cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACgg                                                                         >  1:101736/1‑106 (MQ=255)
                                                                                                            cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCt                              <  2:703762/149‑1 (MQ=255)
                                                                                                             gCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGc                                                                                           >  3:1297014/1‑87 (MQ=255)
                                                                                                             gCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGc                                                                                           <  4:1297014/87‑1 (MQ=255)
                                                                                                              cAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTg                            <  1:564217/149‑1 (MQ=255)
                                                                                                               aaaaTGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGa                           <  2:10984/149‑1 (MQ=255)
                                                                                                                aaaTGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGAt                          <  4:1387477/149‑1 (MQ=255)
                                                                                                                   tgctgcGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGAt                          <  2:479786/146‑1 (MQ=255)
                                                                                                                     ctgcGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTgag                                                                                                     >  1:987551/1‑69 (MQ=255)
                                                                                                                     ctgcGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTgag                                                                                                     <  2:987551/69‑1 (MQ=255)
                                                                                                                     ctgcGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCtt                     <  3:292616/149‑1 (MQ=255)
                                                                                                                       gcgcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTg                                            <  2:1155020/124‑1 (MQ=255)
                                                                                                                       gcgcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTg                                            >  1:1155020/1‑124 (MQ=255)
                                                                                                                         gcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTaaa                 <  4:247936/149‑1 (MQ=255)
                                                                                                                          ccAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                >  3:1385252/1‑149 (MQ=255)
                                                                                                                            aGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCg              >  4:502657/1‑149 (MQ=255)
                                                                                                                             gCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCgg             <  3:1308103/149‑1 (MQ=255)
                                                                                                                              cTGAGCTACAGCGCCTACCATGATGCACTTACGCATCTCGCCAACCGCGCCAGTTTTGAGAAAAAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGt            <  1:1045074/149‑1 (MQ=255)
                                                                                                                              cTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGt            <  1:1351169/149‑1 (MQ=255)
                                                                                                                               tGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTg           >  3:759391/1‑149 (MQ=255)
                                                                                                                                  gCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCtt                             <  1:481291/128‑1 (MQ=255)
                                                                                                                                  gCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCtt                             >  2:481291/1‑128 (MQ=255)
                                                                                                                                  gCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAAt        <  4:1316032/149‑1 (MQ=255)
                                                                                                                                        gcgcCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAgc  <  1:1255089/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                              
TGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGC  >  NC_000913/2145171‑2145455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: