Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 173,746 A→T 7.9% F379L (TTT→TTA hemL ← glutamate‑1‑semialdehyde aminotransferase (aminomutase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913173,7460AT 7.9% 620.2 / 33.5 177F379L (TTT→TTAhemLglutamate‑1‑semialdehyde aminotransferase (aminomutase)
Reads supporting (aligned to +/- strand):  ref base A (80/83);  new base T (7/7);  total (87/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

Only 100 of 188 total aligned reads displayed.

TGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTCCAGCAGACCTTCTGCCAG  >  NC_000913/173598‑173892
                                                                                                                                                    |                                                                                                                                                  
  gATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTaaa                                                                                                                                                  <  3:387679/149‑1 (MQ=255)
  gATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTaaa                                                                                                                                                  >  4:387679/1‑149 (MQ=255)
    tCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGc                                                                                                                                                >  2:1225248/1‑149 (MQ=255)
    tCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGc                                                                                                                                                >  1:177532/1‑149 (MQ=255)
      acaACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGt                                                                                                                                              <  2:1179323/149‑1 (MQ=255)
      acaACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGt                                                                                                                                              <  3:498207/149‑1 (MQ=255)
       caACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGtt                                                                                                                                             <  2:729382/149‑1 (MQ=255)
        aaCTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTc                                                                                                                                            <  1:301533/149‑1 (MQ=255)
          cTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCCTATGGAAGAAACGCTTAAAGCGTTCCa                                                                                                                                          <  3:974814/149‑1 (MQ=255)
             cGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGt                                                                                                                                       <  1:1225248/149‑1 (MQ=255)
                          cGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATcac                                                                                                                          <  1:609144/149‑1 (MQ=255)
                                 caTCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGa                                                                                                                   >  1:175851/1‑149 (MQ=255)
                                  aTCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGa                                                                                                                   <  4:1465695/148‑1 (MQ=255)
                                  aTCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGa                                                                                                                   >  3:1465695/1‑148 (MQ=255)
                                  aTCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGAt                                                                                                                  >  4:822168/1‑149 (MQ=255)
                                   tCGATGGTGTTATTGATATCTTCCATGCTGTGCGCTACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATa                                                                                                                 <  4:952035/149‑1 (MQ=255)
                                     gATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGGCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGc                                                                                                               >  2:1297908/1‑149 (MQ=255)
                                          tgtTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGtt                                                                                                                                             <  2:720234/114‑1 (MQ=255)
                                          tgtTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGtt                                                                                                                                             >  1:720234/1‑114 (MQ=255)
                                              aTTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTaaa                                                                                                                                                  <  3:891832/105‑1 (MQ=255)
                                               ttGATATCTTCCATGCTGTGCGCCACGGACGTAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGa                                                                                                     <  1:318989/149‑1 (MQ=255)
                                                tGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGAc                                                                                                    >  1:1038150/1‑149 (MQ=255)
                                                  atatCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTc                                                                                                  >  3:1153865/1‑149 (MQ=255)
                                                   tatCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCg                                                                                                 <  4:976581/149‑1 (MQ=255)
                                                   tatCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCg                                                                                                 <  3:563324/149‑1 (MQ=255)
                                                       ttCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTTAAGCGtt                                                                                                                                             <  3:996230/101‑1 (MQ=255)
                                                       ttCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGa                                                                                                                   <  3:596472/127‑1 (MQ=255)
                                                         ccATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGCCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCg                                                                                           <  1:1297908/149‑1 (MQ=255)
                                                           aTGCTGCGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGt                                                                                         >  2:1040700/1‑149 (MQ=255)
                                                              cTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCGCGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTaaa                                                                                      <  1:171660/149‑1 (MQ=255)
                                                               tgtgCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAg                                                                                     <  2:175851/149‑1 (MQ=255)
                                                                   cgcCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGaaaa                                                                                 <  1:697886/149‑1 (MQ=255)
                                                                    gcCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAAt                                                                                >  2:327570/1‑149 (MQ=255)
                                                                      catggACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATAc                                                                              <  3:1291301/146‑1 (MQ=255)
                                                                      cACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATAc                                                                              >  3:263977/1‑149 (MQ=255)
                                                                         ggACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTa                                                                                                                                                    >  1:1017379/1‑76 (MQ=255)
                                                                              tagaGCACGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAg                                                                                     <  4:58040/131‑1 (MQ=255)
                                                                              tAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATg                                                                      >  2:172474/1‑149 (MQ=255)
                                                                               aaaGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGa                                                                                                                   >  2:1074585/1‑103 (MQ=255)
                                                                               aaaGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCAc                                                                                                        >  2:614338/1‑114 (MQ=255)
                                                                               aaaGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCAc                                                                                                        <  1:614338/114‑1 (MQ=255)
                                                                                aaGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGa                                                                                                     <  2:54643/116‑1 (MQ=255)
                                                                                aaGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCAAATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAGAATACCGAACATgcc                                                                    >  2:423723/1‑149 (MQ=255)
                                                                                  gCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATgccgc                                                                  >  1:491315/1‑149 (MQ=255)
                                                                                     cGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTTAAGCGt                                                                                                                                              >  2:229700/1‑70 (MQ=255)
                                                                                      gCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAAc                                                              <  4:1153865/149‑1 (MQ=255)
                                                                                       cTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGc                                                                                                                                >  4:1249580/1‑82 (MQ=255)
                                                                                          cAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTcac                                                                                                                                    >  2:75828/1‑75 (MQ=255)
                                                                                          cAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTcac                                                                                                                                    <  1:75828/75‑1 (MQ=255)
                                                                                           aaaCGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATGATAGGGAAGAAACGCTCAAAGCGTTCCACGTCACAGACCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTGAAGAAAATACCGAACATGCCGCGAACGTGGt                                                         >  3:1226740/1‑149 (MQ=255)
                                                                                            aaCGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGtt                                                        >  4:59782/1‑149 (MQ=255)
                                                                                            aaCGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGtt                                                        >  2:699392/1‑149 (MQ=255)
                                                                                                 tGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGaa                                                                                   <  3:841252/117‑1 (MQ=255)
                                                                                                  gACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCa                                                                                                                                          <  4:1402655/61‑1 (MQ=255)
                                                                                                  gACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCa                                                                                                                                          >  3:1402655/1‑61 (MQ=255)
                                                                                                  gACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTCAACGAc                                                  <  2:1038150/149‑1 (MQ=255)
                                                                                                        gCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCCGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCgg                                            >  4:869238/1‑149 (MQ=255)
                                                                                                          cAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAATATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGaa                                          <  1:423723/149‑1 (MQ=255)
                                                                                                           aGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTgg                                                          >  2:848478/1‑132 (MQ=255)
                                                                                                           aGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTgg                                                          >  4:234867/1‑132 (MQ=255)
                                                                                                           aGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTgg                                                          <  1:848478/132‑1 (MQ=255)
                                                                                                               aaaCACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCg                                                                                              >  4:755336/1‑92 (MQ=255)
                                                                                                               aaaCACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCg                                                                                              <  3:755336/92‑1 (MQ=255)
                                                                                                               aaaCACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGc                                              >  2:901271/1‑140 (MQ=255)
                                                                                                               aaaCACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGc                                              <  1:901271/140‑1 (MQ=255)
                                                                                                                 acacCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCg                                                                                           <  4:466549/93‑1 (MQ=255)
                                                                                                                 acacCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCg                                                                                           >  3:466549/1‑93 (MQ=255)
                                                                                                                 acacCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGaa                                                                          >  4:525328/1‑110 (MQ=255)
                                                                                                                     cTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGaaaa                                                                                 <  3:798037/99‑1 (MQ=255)
                                                                                                                     cTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATAc                                                                              <  3:199198/102‑1 (MQ=255)
                                                                                                                     cTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGaa                                                                          >  3:273525/1‑106 (MQ=255)
                                                                                                                     cTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGaa                                                                          <  4:273525/106‑1 (MQ=255)
                                                                                                                     cTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGcttct                               >  4:303716/1‑149 (MQ=255)
                                                                                                                      ttCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGcttctt                              >  4:1287368/1‑149 (MQ=255)
                                                                                                                         gTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTg                           >  4:746135/1‑149 (MQ=255)
                                                                                                                             aGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGAc                                                  >  1:530968/1‑122 (MQ=255)
                                                                                                                                   atatGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTCcag                 >  1:994756/1‑149 (MQ=255)
                                                                                                                                    tatGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGt                                                            <  3:692707/105‑1 (MQ=255)
                                                                                                                                    tatGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGt                                                            >  4:692707/1‑105 (MQ=255)
                                                                                                                                      tGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTCcagcag              >  4:359048/1‑149 (MQ=255)
                                                                                                                                        gaagaaACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGcttc                                >  4:797038/1‑129 (MQ=255)
                                                                                                                                          agaaACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTCCAGCAGACCt          >  3:1179387/1‑149 (MQ=255)
                                                                                                                                          agaaACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTCCAGCAGACCt          >  2:1351693/1‑149 (MQ=255)
                                                                                                                                          agaaACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTCCAGCAGACCt          >  4:731870/1‑149 (MQ=255)
                                                                                                                                           ggaaCGCTTTAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCgg                                                                                          <  4:1217212/66‑1 (MQ=255)
                                                                                                                                            gaaCGCTTTAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATAc                                                                              <  3:1060573/78‑1 (MQ=255)
                                                                                                                                             aaCGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTg                                                           <  1:892724/97‑1 (MQ=255)
                                                                                                                                             aaCGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTg                                                           >  2:892724/1‑97 (MQ=255)
                                                                                                                                             aaCGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTCCAGCAGACCTTCt       <  2:491315/149‑1 (MQ=255)
                                                                                                                                              aCGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTgg                                                          <  1:1069806/97‑1 (MQ=255)
                                                                                                                                              aCGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTgg                                                          <  3:733980/97‑1 (MQ=255)
                                                                                                                                              aCGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTCCAGCAGACCTTc        <  2:1415025/147‑1 (MQ=255)
                                                                                                                                              aCGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTCCAGCAGACCTTCTg      >  1:1149054/1‑149 (MQ=255)
                                                                                                                                                gCTTTAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGtt                                                        >  4:621897/1‑97 (MQ=255)
                                                                                                                                                gCTTTAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGtt                                                        <  3:621897/97‑1 (MQ=255)
                                                                                                                                                gCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATgcc                                                                    >  1:1379872/1‑85 (MQ=255)
                                                                                                                                                  ttAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGc                                              >  4:1137118/1‑105 (MQ=255)
                                                                                                                                                  ttAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGc                                              <  3:1137118/105‑1 (MQ=255)
                                                                                                                                                  ttAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTc                    <  3:1270354/131‑1 (MQ=255)
                                                                                                                                                  ttAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTCCAGCAGACCTTCTGCCAg  >  1:36194/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGTCCAGCATCATATGGAAGAAACGCTTAAAGCGTTCCACGTCACAGGCCATCACATCCTGATAGCACGTCACGGACTCGGCGTCGGTAAAGAAAATACCGAACATGCCGCCAACGTGGTTAACGACCAGCGGAATTCCGGCTTCTTCTGCCGCTTCCAGCAGACCTTCTGCCAG  >  NC_000913/173598‑173892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: