Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,284,338 C→T 8.0% N70N (AAC→AAT yejL → UPF0352 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,284,3380CT 8.0% 636.3 / 34.1 199N70N (AAC→AATyejLUPF0352 family protein
Reads supporting (aligned to +/- strand):  ref base C (95/88);  new base T (8/8);  total (103/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 206 total aligned reads displayed.

ACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAATATTCTGCTTTCGCTCGTCATTGGCAGCCGTTAC  >  NC_000913/2284190‑2284486
                                                                                                                                                    |                                                                                                                                                    
      tGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGAc                                                                                                                                                >  4:1120550/1‑149 (MQ=255)
                tAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGAc                                                                                                                                                >  2:234040/1‑139 (MQ=255)
                    gCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAgg                                                                                                                                  >  3:1441525/1‑149 (MQ=255)
                     cTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAATGAAGACAAAGCGCACTAAggg                                                                                                                                 <  3:925452/149‑1 (MQ=255)
                      tCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACaaa                                                                                                                                             >  3:180193/1‑136 (MQ=255)
                      tCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACaaa                                                                                                                                             <  4:180193/136‑1 (MQ=255)
                         gaccgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAATGAAGACAAAGCGCACTAAGGGGAAc                                                                                                                             >  3:649481/1‑149 (MQ=255)
                          accgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCAc                                                                                                                                       <  2:1349577/138‑1 (MQ=255)
                            cgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGa                                                                                                                          <  1:650543/149‑1 (MQ=255)
                              accTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAATGAAGACAAAGCGCACTAAGGGAAACAGATa                                                                                                                        >  4:962954/1‑149 (MQ=255)
                              accTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAATGAAGACAAAGCGCACTAAGGGAAACAGATa                                                                                                                        >  4:928058/1‑149 (MQ=255)
                                 tttCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACa                                                                                                                     >  3:15454/1‑149 (MQ=255)
                                 tttCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACa                                                                                                                     <  2:729482/149‑1 (MQ=255)
                                  ttCCCCGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAg                                                                                                                    <  3:235429/149‑1 (MQ=255)
                                      cTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGGACTAAGGGAAACAGATAACAGGTTa                                                                                                                <  4:1371171/149‑1 (MQ=255)
                                       tGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAATGAAGACAAAGCGCACTAAGGGGAACAGATAACAGGTTAt                                                                                                               <  4:649481/149‑1 (MQ=255)
                                           ggTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTa                                                                                                           >  1:565543/1‑149 (MQ=255)
                                               ttAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTc                                                                                                       >  2:663399/1‑149 (MQ=255)
                                                   gCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATaa                                                                                                                       >  1:1014791/1‑129 (MQ=255)
                                                     aaCATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATgg                                                                                                             <  1:85099/137‑1 (MQ=255)
                                                     aaCATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATgg                                                                                                             >  2:85099/1‑137 (MQ=255)
                                                     aaCATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTc                                                                                                 >  3:529951/1‑149 (MQ=255)
                                                     aaCATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTc                                                                                                 <  4:239839/149‑1 (MQ=255)
                                                     aaCATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTc                                                                                                 >  3:993761/1‑149 (MQ=255)
                                                     aaCATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTc                                                                                                 >  4:1068543/1‑149 (MQ=255)
                                                      aCATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAg                                                                                                                    >  3:1055957/1‑129 (MQ=255)
                                                        aTGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAgc                                                                                              <  3:1120550/149‑1 (MQ=255)
                                                         tGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAgcg                                                                                             >  4:1092918/1‑149 (MQ=255)
                                                           gTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCt                                                                                           >  4:1344693/1‑149 (MQ=255)
                                                             cACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTc                                                                                                       <  1:750754/135‑1 (MQ=255)
                                                              aCTAACCTTATCAACACCAGTATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTAcc                                                                                        >  3:1230066/1‑149 (MQ=255)
                                                              aCTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAAc                                                                                                                                                      >  2:523637/1‑87 (MQ=255)
                                                                tAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAggg                                                                                                                                 >  1:326825/1‑106 (MQ=255)
                                                                tAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGt                                                                                      >  1:521366/1‑149 (MQ=255)
                                                                tAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATCGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATc                                                                                                    >  2:762049/1‑135 (MQ=255)
                                                                 aaCCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCTGCGCCTGACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAgg                                                                                                                   <  3:1418913/119‑1 (MQ=255)
                                                                  aCCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGa                                                                                    >  1:678156/1‑149 (MQ=255)
                                                                  aCCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGa                                                                                    >  1:1223465/1‑149 (MQ=255)
                                                                   ccTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGaa                                                                                   >  1:484743/1‑149 (MQ=255)
                                                                     ttATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGa                                                                                                                                                 >  3:809007/1‑85 (MQ=255)
                                                                       aTCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGa                                                                                                                                                 <  3:872801/83‑1 (MQ=255)
                                                                        tCAACACCAGTATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGt                                                                              <  4:1230066/149‑1 (MQ=255)
                                                                        tCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGa                                                                                                                                                 >  1:139940/1‑82 (MQ=255)
                                                                           acacCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGtctc                                                                           >  2:1410962/1‑149 (MQ=255)
                                                                           acacCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGAGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGtctc                                                                           <  1:111388/149‑1 (MQ=255)
                                                                             acCAGCATTGCCCCGGCCGAACGCCAGGCAATTGCCAACGCTGTTGCCCGCGACTTACACTCCTCTATCAACGAAGACAAAGCGCACTAAAGGAAACAGGTAACAGGTTATGGTTACTCATCGTCAGCGCTACCGTGAAAAAGTCTccc                                                                         <  2:642168/149‑1 (MQ=255)
                                                                             acCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTccc                                                                         <  2:1223465/149‑1 (MQ=255)
                                                                             acCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTccc                                                                         <  3:229537/149‑1 (MQ=255)
                                                                              ccAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGaaa                                                                                                                              >  3:337303/1‑95 (MQ=255)
                                                                              ccAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATc                                                                                                    <  4:802232/121‑1 (MQ=255)
                                                                               cAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTcc                                                                          <  2:868983/146‑1 (MQ=255)
                                                                               cAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTcc                                                                          >  1:868983/1‑146 (MQ=255)
                                                                                 gCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAAc                                                                                                                                                      <  4:148452/68‑1 (MQ=255)
                                                                                  cATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATg                                                                    <  3:1344693/149‑1 (MQ=255)
                                                                                    ttGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAg                                                                                                                                   <  4:896493/84‑1 (MQ=255)
                                                                                      gCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCa                                                                <  3:1068543/149‑1 (MQ=255)
                                                                                        cccGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCGACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGt                                                              >  1:644528/1‑149 (MQ=255)
                                                                                           ggCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTgg                                                           >  2:785101/1‑149 (MQ=255)
                                                                                              ccAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAATGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAAc                                                                                                         >  1:780089/1‑100 (MQ=255)
                                                                                              ccAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGtct                                                                            >  4:1098873/1‑129 (MQ=255)
                                                                                              ccAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGtct                                                                            <  3:1098873/129‑1 (MQ=255)
                                                                                                 aCGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAATGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCAc                                                     <  3:962954/149‑1 (MQ=255)
                                                                                                    ccAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTgg                                                  <  1:633870/149‑1 (MQ=255)
                                                                                                      aGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGtct                                                                            <  1:197449/121‑1 (MQ=255)
                                                                                                      aGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGtt                                                >  3:323135/1‑149 (MQ=255)
                                                                                                       ggCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGttt                                               <  1:41905/149‑1 (MQ=255)
                                                                                                          aaTTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGa                                                                                                                          >  4:301630/1‑71 (MQ=255)
                                                                                                          aaTTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGa                                                                                                                          <  3:245223/71‑1 (MQ=255)
                                                                                                          aaTTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCa                                            <  4:529951/149‑1 (MQ=255)
                                                                                                               ccAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGtt                                       >  4:760234/1‑149 (MQ=255)
                                                                                                               ccAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGtt                                       <  3:1092918/149‑1 (MQ=255)
                                                                                                                cAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAAc                                                                                                                             >  1:815718/1‑62 (MQ=255)
                                                                                                                cAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATc                                                                                                    <  2:722560/87‑1 (MQ=255)
                                                                                                                cAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTggg                                                          >  4:552037/1‑129 (MQ=255)
                                                                                                                cAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTggg                                                          <  3:552037/129‑1 (MQ=255)
                                                                                                                 aaCTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCa                                     >  2:482601/1‑149 (MQ=255)
                                                                                                                 aaCTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCa                                     >  1:1332003/1‑149 (MQ=255)
                                                                                                                    tctTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAata                                  >  3:204586/1‑149 (MQ=255)
                                                                                                                      ttttGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATTGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAATAtt                                <  2:362994/149‑1 (MQ=255)
                                                                                                                      ttttGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTggg                                                          >  4:1038598/1‑123 (MQ=255)
                                                                                                                      ttttGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGtt                                                <  4:323416/133‑1 (MQ=255)
                                                                                                                      ttttGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGc                                             >  2:836273/1‑136 (MQ=255)
                                                                                                                                 ccTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAATATTCTGCTTTCGCt                     <  3:760234/149‑1 (MQ=255)
                                                                                                                                  cTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAATATTCTGCTTTc                        >  4:859989/1‑145 (MQ=255)
                                                                                                                                       aGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGt                                                                  >  1:1150225/1‑98 (MQ=255)
                                                                                                                                       aGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGt                                                                  <  2:1150225/98‑1 (MQ=255)
                                                                                                                                         tCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGaaaaa                                                                                >  4:232076/1‑82 (MQ=255)
                                                                                                                                         tCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTggg                                                          >  1:83846/1‑104 (MQ=255)
                                                                                                                                         tCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAATATTCTGCt                           >  2:1191858/1‑135 (MQ=255)
                                                                                                                                         tCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAATATTCTGCt                           <  1:1191858/135‑1 (MQ=255)
                                                                                                                                         tCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAATATTCTGCTTTCGCTCGTCATTg             >  4:1188731/1‑149 (MQ=255)
                                                                                                                                           ctctATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAATATTCTGCTTTCGCTCGTCATTGGc           >  2:591438/1‑149 (MQ=255)
                                                                                                                                              tATCAATGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTc                                      >  4:205959/1‑119 (MQ=255)
                                                                                                                                              tATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGtct                                                                            <  2:1047918/81‑1 (MQ=255)
                                                                                                                                                tCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTggg                                                          >  1:885643/1‑97 (MQ=255)
                                                                                                                                                tCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAATATTCTGCTTTCGCTCGTCATTGGCAGCCg      >  4:372231/1‑149 (MQ=255)
                                                                                                                                                   aCGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGt                                                                                                            <  4:1423661/44‑1 (MQ=255)
                                                                                                                                                   aCGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGtt                                       <  3:1302377/113‑1 (MQ=255)
                                                                                                                                                   aCGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGtt                                       >  4:1302377/1‑113 (MQ=255)
                                                                                                                                                   aCGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGtt                                       <  3:1035723/113‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAATATTCTGCTTTCGCTCGTCATTGGCAGCCGTTAC  >  NC_000913/2284190‑2284486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: