| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,329,710 | C→T | 10.2% | G30S (GGT→AGT) ‡ | yfaQ ← | tandem DUF2300 domain protein, putative host defense protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,329,710 | 0 | C | T | 10.2% | 175.9 / 9.3 | 59 | G30S (GGT→AGT) ‡ | yfaQ | tandem DUF2300 domain protein, putative host defense protein |
| Reads supporting (aligned to +/- strand): ref base C (25/28); new base T (4/2); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCGGTCCATTCACTCCCTGCCGCAACACTTTGCTGCGCAGGTGCA > NC_000913/2329575‑2329858 | gATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGt < 1:503198/149‑1 (MQ=255) tATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAAc > 1:885642/1‑149 (MQ=255) tATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTAATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAAc > 4:766303/1‑149 (MQ=255) tccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcgcg < 2:1166163/128‑1 (MQ=255) tccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcgcg > 1:1166163/1‑128 (MQ=255) ccagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGCCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGtc < 1:1463906/149‑1 (MQ=255) cagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTAATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcctc < 3:766303/149‑1 (MQ=255) aTACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCGGTGCGCAGCAc < 4:968928/117‑1 (MQ=255) aTACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCGGTGCGCAGCAc > 3:968928/1‑117 (MQ=255) aTACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTg < 2:885642/149‑1 (MQ=255) aCAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGTGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTg < 1:776388/147‑1 (MQ=255) aCAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGTGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTg > 2:776388/1‑147 (MQ=255) tttCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTAGCagag > 3:625365/1‑149 (MQ=255) acaACCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCagag < 2:1359000/144‑1 (MQ=255) acaAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCagag > 1:1359000/1‑144 (MQ=255) cTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGc < 1:1398692/149‑1 (MQ=255) tCCCTAACGGCGTGGTGAGCGAGTCAGGTAATTCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcgc < 2:1179593/93‑1 (MQ=255) tCCCTAACGGCGTGGTGAGCGAGTCAGGTAATTCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcgc > 1:1179593/1‑93 (MQ=255) cccTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcg < 4:1181202/91‑1 (MQ=255) cccTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcg > 3:1181202/1‑91 (MQ=255) ccTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTaa < 2:1423865/139‑1 (MQ=255) ccTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTaa > 1:1423865/1‑139 (MQ=255) ggCGTGGTGAGGGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCGGTGCgcagc > 3:1177660/1‑90 (MQ=255) tCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGc > 3:1236241/1‑90 (MQ=255) tCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGc < 4:1236241/90‑1 (MQ=255) tCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGa > 2:267322/1‑139 (MQ=255) tCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGa < 1:267322/139‑1 (MQ=255) tCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCa > 3:84417/1‑149 (MQ=255) gCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcct < 2:568166/88‑1 (MQ=255) gCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcct > 1:568166/1‑88 (MQ=255) cTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTAt < 2:1002966/149‑1 (MQ=255) cTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTAt > 1:1002966/1‑149 (MQ=255) gagaCTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTAttt > 1:1450397/1‑149 (MQ=255) agaCTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCaa > 2:635288/1‑90 (MQ=255) agaCTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCaa < 1:635288/90‑1 (MQ=255) aCTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTAGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCAc < 4:625365/149‑1 (MQ=255) tttGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCaa > 2:241191/1‑86 (MQ=255) tttGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCaa < 1:241191/86‑1 (MQ=255) tttGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGAc > 3:1009968/1‑120 (MQ=255) tttGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGAc < 4:1009968/120‑1 (MQ=255) gacAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCa > 4:14082/1‑127 (MQ=255) gacAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCa < 3:14082/127‑1 (MQ=255) acAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACTGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCAc < 1:1111989/127‑1 (MQ=255) acAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACTGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCAc > 2:1111989/1‑127 (MQ=255) gCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTc < 2:915886/102‑1 (MQ=255) gCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTc > 1:915886/1‑102 (MQ=255) gCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCgg < 4:152398/135‑1 (MQ=255) gCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCgg > 3:152398/1‑135 (MQ=255) gTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGAt > 2:458351/1‑91 (MQ=255) gTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGAt < 1:458351/91‑1 (MQ=255) ttGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCGGTCCATTCACTCCCTGCCGCAacac < 4:84417/149‑1 (MQ=255) actggTGCTGCGCGGTGCGCAGCACGAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTAtt > 4:1115439/5‑108 (MQ=38) aTCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgtgt > 3:1410916/1‑54 (MQ=255) aTCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgtgt < 4:1410916/54‑1 (MQ=255) gTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCGGTCCATTCACTCCCTGCCGCAACACTTtgct < 2:1450397/149‑1 (MQ=255) tgctgcGCGGTGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGAc < 3:35093/80‑1 (MQ=255) tgctgcGCGGTGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGAc > 4:35093/1‑80 (MQ=255) ctgcGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCAc > 3:1028485/1‑105 (MQ=255) ctgcGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCAc < 4:1028485/105‑1 (MQ=255) tgcGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAg < 1:1090593/90‑1 (MQ=255) tgcGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAg > 2:1090593/1‑90 (MQ=255) tgcGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCt < 1:630729/106‑1 (MQ=255) tgcGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCt > 2:630729/1‑106 (MQ=255) tgcGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCGGTCCATTCACTCCCTGCCGCaa > 4:708053/1‑136 (MQ=255) tgcGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCGGTCCATTCACTCCCTGCCGCaa < 3:708053/136‑1 (MQ=255) cgcgCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCGGTCCATTCACTCCCTGc < 1:186816/124‑1 (MQ=255) cgcgCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCGGTCCATTCACTCCCTGc > 2:186816/1‑124 (MQ=255) cgcgCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCGGTCCATTCACTCCCTGCCGCAACACTTTGCTGCGCAGGTGCa > 3:1341742/1‑149 (MQ=255) cgcgCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCGGTCCATTCACTACCTGc > 4:1433437/1‑124 (MQ=255) cgcgCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCGGTCCATTCACTACCTGc < 3:1433437/124‑1 (MQ=255) | GATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGATTCTGCGCCAGTTCACTTATTTCACCTGCATCCCGGTCCATTCACTCCCTGCCGCAACACTTTGCTGCGCAGGTGCA > NC_000913/2329575‑2329858 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |