Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,340,372 A→G 7.9% intergenic (+83/+45) ubiG → / ← yfaL bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,340,3720AG 7.9% 204.9 / 6.0 63intergenic (+83/+45)ubiG/yfaLbifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin
Reads supporting (aligned to +/- strand):  ref base A (30/28);  new base G (2/3);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATAT  >  NC_000913/2340226‑2340520
                                                                                                                                                  |                                                                                                                                                    
tAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCATTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAg                                                                                                                                                    <  4:999427/149‑1 (MQ=255)
tAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAg                                                                                                                                                    >  3:999427/1‑149 (MQ=255)
tAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAg                                                                                                                                                    <  3:746719/149‑1 (MQ=255)
tAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAg                                                                                                                                                    <  3:425208/149‑1 (MQ=255)
  aTACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGc                                                                                                                                                  <  2:1100544/149‑1 (MQ=255)
      ttttAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATaa                                                                                                                                                     >  1:1887/1‑142 (MQ=255)
      ttttAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATaa                                                                                                                                                     <  2:1887/142‑1 (MQ=255)
                  ccccGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                                  <  3:825510/149‑1 (MQ=38)
                                  aaCTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGt                                                                                                                          >  2:792114/1‑141 (MQ=255)
                                  aaCTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGt                                                                                                                          <  1:792114/141‑1 (MQ=255)
                                    cTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgcctta                                                                                                                                      >  4:1124813/1‑124 (MQ=38)
                                    cTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgcctta                                                                                                                                      <  3:1124813/127‑4 (MQ=38)
                                     tataTGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgcctta                                                                                                                                      >  1:816403/1‑123 (MQ=38)
                                     tataTGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgcctta                                                                                                                                      <  2:816403/126‑4 (MQ=38)
                                                  cGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAtt                                                                                                  >  4:489631/1‑149 (MQ=255)
                                                   gCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCg                                                                                                                                                 <  4:1046682/101‑1 (MQ=255)
                                                   gCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCg                                                                                                                                                 >  3:1046682/1‑101 (MQ=255)
                                                           aGTGAGGGTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCa                                                                                         >  2:1294057/1‑149 (MQ=255)
                                                                 gTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGc                                                                                                                                                  <  2:294228/86‑1 (MQ=255)
                                                                 gTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGc                                                                                                                                                  >  1:294228/1‑86 (MQ=255)
                                                                      tGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAAc                                                                              <  3:489631/149‑1 (MQ=255)
                                                                        tttGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAAccc                                                                            <  1:1294057/149‑1 (MQ=255)
                                                                            gCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGa                                                                                                          <  3:1106581/115‑1 (MQ=255)
                                                                            gCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGa                                                                                                          >  4:1106581/1‑115 (MQ=255)
                                                                               gcgcCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTc                                                                     >  3:1036607/1‑149 (MQ=255)
                                                                                   cAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                                                                                                                              >  2:1071850/1‑88 (MQ=255)
                                                                                   cAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                                                                                                                              <  1:1071850/88‑1 (MQ=255)
                                                                                    aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGt                                                                                                            <  2:1334579/105‑1 (MQ=255)
                                                                                    aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGt                                                                                                            >  1:1334579/1‑105 (MQ=255)
                                                                                        ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCTGACTAGACAGTGa                                                                                                          >  1:704878/1‑103 (MQ=255)
                                                                                        ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCTGACTAGACAGTGa                                                                                                          <  2:704878/103‑1 (MQ=255)
                                                                                               cGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCAc                                                     >  4:480473/1‑149 (MQ=255)
                                                                                                       gCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAAccc                                                                            <  1:1290587/118‑1 (MQ=255)
                                                                                                       gCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAAccc                                                                            >  2:1290587/1‑118 (MQ=255)
                                                                                                        ccGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTgg                                                                  >  2:827921/1‑127 (MQ=255)
                                                                                                        ccGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTgg                                                                  <  1:827921/127‑1 (MQ=255)
                                                                                                        aagcgTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCt                                            <  4:1358233/147‑1 (MQ=255)
                                                                                                         cgcgTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTg                                           >  2:686632/1‑149 (MQ=255)
                                                                                                             tCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGtt                                                                                                                                               >  2:196414/1‑45 (MQ=255)
                                                                                                             tCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGtt                                                                                                                                               <  1:196414/45‑1 (MQ=255)
                                                                                                               ttATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGt                                                                                           <  1:1172600/95‑1 (MQ=255)
                                                                                                               ttATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGt                                                                                           >  2:1172600/1‑95 (MQ=255)
                                                                                                                tATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTggg                                                                 <  2:715665/120‑1 (MQ=255)
                                                                                                                tATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTggg                                                                 >  1:715665/1‑120 (MQ=255)
                                                                                                                 aTCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccacgcca                                   >  2:1443778/1‑149 (MQ=255)
                                                                                                                  tCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccacgccac                                  >  2:94745/1‑149 (MQ=255)
                                                                                                                    aGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAt                                                                                                   >  1:875945/1‑82 (MQ=255)
                                                                                                                    aGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAt                                                                                                   <  2:875945/82‑1 (MQ=255)
                                                                                                                         tACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGa                                                                                                                <  3:1283081/64‑1 (MQ=255)
                                                                                                                         tACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGa                                                                                                                >  4:1283081/1‑64 (MQ=255)
                                                                                                                                gCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTg                                                                   >  4:831162/1‑102 (MQ=255)
                                                                                                                                gCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTg                                                                   <  3:831162/102‑1 (MQ=255)
                                                                                                                                gCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCt                                            <  4:729136/125‑1 (MQ=255)
                                                                                                                                gCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCt                                            >  3:729136/1‑125 (MQ=255)
                                                                                                                                 cTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGa                   <  1:686632/149‑1 (MQ=255)
                                                                                                                                  tCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGac                  >  2:224671/1‑149 (MQ=255)
                                                                                                                                  tCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGac                  >  4:444291/1‑149 (MQ=255)
                                                                                                                                    cccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACaaaaa                                                                               <  3:1370717/86‑1 (MQ=255)
                                                                                                                                    cccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACaaaaa                                                                               >  4:1370717/1‑86 (MQ=255)
                                                                                                                                           gCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctga         <  1:1443778/149‑1 (MQ=255)
                                                                                                                                            ccGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCgcgc                        <  4:572382/133‑1 (MQ=255)
                                                                                                                                            ccGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCgcgc                        >  3:572382/1‑133 (MQ=255)
                                                                                                                                               gATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCCCGCCACGCTGCCGCGCAGGGACACTCGctgact       >  2:727258/1‑147 (MQ=255)
                                                                                                                                               gATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctgactga     >  1:639315/1‑149 (MQ=255)
                                                                                                                                               gATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctgact       <  1:727258/147‑1 (MQ=255)
                                                                                                                                                  aaGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGatat  >  4:145106/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATAT  >  NC_000913/2340226‑2340520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: