Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,400,684 T→A 5.4% Q291L (CAG→CTG)  nuoF ← NADH:ubiquinone oxidoreductase, chain F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,400,6840TA 5.4% 534.6 / 15.0 149Q291L (CAG→CTG) nuoFNADH:ubiquinone oxidoreductase, chain F
Reads supporting (aligned to +/- strand):  ref base T (71/68);  new base A (3/5);  total (75/74)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 149 total aligned reads displayed.

ACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCTTT  >  NC_000913/2400540‑2400829
                                                                                                                                                |                                                                                                                                                 
aCCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCa                                                                                                                                               >  1:1139127/1‑149 (MQ=255)
 ccAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAg                                                                                                                                              >  4:154275/1‑149 (MQ=255)
    gCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCt                                                                                                                                           >  1:102656/1‑149 (MQ=255)
    gCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCt                                                                                                                                           >  2:555787/1‑149 (MQ=255)
    gCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCAGCCAGGcg                                                                                                                                           <  3:1379526/149‑2 (MQ=255)
       acacCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTa                                                                                                                                        <  4:1363247/149‑1 (MQ=255)
           cATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATAGTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAtt                                                                                                                                    >  1:428753/1‑149 (MQ=255)
            aTGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAttt                                                                                                                                   <  4:842559/149‑1 (MQ=255)
             tGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGcc                                                                                                                                                >  3:1325772/1‑135 (MQ=255)
             tGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGcc                                                                                                                                                <  4:1325772/135‑1 (MQ=255)
              gTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCa                                                                                                                                 >  3:1139035/1‑149 (MQ=255)
               ttGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGc                                                                                                                                                 >  3:348935/1‑132 (MQ=255)
               ttGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGc                                                                                                                                                 <  4:348935/132‑1 (MQ=255)
                tGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGa                                                                                                                               <  2:1139127/149‑1 (MQ=255)
                tGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGa                                                                                                                               <  2:1265026/149‑1 (MQ=255)
                   tctcATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAtt                                                                                                                                    <  2:1433319/141‑1 (MQ=255)
                   tctcATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCa                                                                                                                            <  3:108563/149‑1 (MQ=255)
                     tcATGGTGAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCGGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTaa                                                                                                                                       >  4:1240788/1‑136 (MQ=255)
                     tcATGGTCAACCGCCATCGCCAGCGCCGTACCCATACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTaa                                                                                                                                       <  3:1240788/136‑1 (MQ=255)
                      cATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCa                                                                                                                         <  1:326308/149‑1 (MQ=255)
                           tCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCa                                                                                                                                 <  3:454120/136‑1 (MQ=255)
                             aaCCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATac                                                                                                                  >  3:445567/1‑149 (MQ=255)
                                   cATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCa                                                                                                                     >  1:42677/1‑140 (MQ=255)
                                     tCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCtt                                                                                                                                          <  1:1390916/117‑1 (MQ=255)
                                             gcCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTc                                                                                                  >  1:149286/1‑149 (MQ=255)
                                                     cAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGAtct                                                                                          >  3:96403/1‑149 (MQ=255)
                                                          ggCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGAt                                                                                            >  1:1465960/1‑142 (MQ=255)
                                                          ggCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGAt                                                                                            <  2:1465960/142‑1 (MQ=255)
                                                          ggCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGt                                                                                     >  2:1072344/1‑149 (MQ=255)
                                                               cccGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggt                                                                                <  3:154275/149‑1 (MQ=255)
                                                                 cGCTTTACCGATAGTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtgg                                                                              <  2:428753/149‑1 (MQ=255)
                                                                 cGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCa                                                                                                                     >  4:1130785/1‑110 (MQ=255)
                                                                 cGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCa                                                                                                                     <  1:1174394/110‑1 (MQ=255)
                                                                 cGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCa                                                                                                                     >  2:1174394/1‑110 (MQ=255)
                                                                     ttACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGcc                                                                          >  3:821320/1‑149 (MQ=255)
                                                                        ccGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGaa                                                                       <  4:742447/149‑1 (MQ=255)
                                                                         cGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTaaa                                                                                                                                      <  1:370779/85‑1 (MQ=255)
                                                                         cGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGc                                                                                                                      <  2:332770/101‑1 (MQ=255)
                                                                         cGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGc                                                                                                                      >  1:332770/1‑101 (MQ=255)
                                                                          gATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCa                                                                                                                                 <  3:54796/89‑1 (MQ=255)
                                                                           aTACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAt                                                                                                                                     <  1:506465/84‑1 (MQ=255)
                                                                               tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGa                                                                                                <  1:59728/117‑1 (MQ=255)
                                                                               tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGa                                                                                                >  2:59728/1‑117 (MQ=255)
                                                                               tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGa                                                                                             >  2:656606/1‑120 (MQ=255)
                                                                               tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCa                                                                  >  4:1117614/1‑147 (MQ=255)
                                                                               tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCa                                                                  <  3:243494/147‑1 (MQ=255)
                                                                               tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCa                                                                  <  3:1117614/147‑1 (MQ=255)
                                                                               tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCa                                                                  >  4:557512/1‑147 (MQ=255)
                                                                               tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCa                                                                  <  3:557512/147‑1 (MQ=255)
                                                                               tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCa                                                                  >  4:243494/1‑147 (MQ=255)
                                                                                  cGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTcc                                                             <  4:96403/149‑1 (MQ=255)
                                                                                    aaTTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCca                                                           <  4:445567/149‑1 (MQ=255)
                                                                                     aTTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCcac                                                          >  1:1309446/1‑149 (MQ=255)
                                                                                      ttCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTTAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCcaca                                                         >  4:1211938/1‑149 (MQ=255)
                                                                                      ttCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtggt                                                                             >  4:538358/1‑129 (MQ=255)
                                                                                      ttCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtggt                                                                             <  3:538358/129‑1 (MQ=255)
                                                                                         cATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCa                                                                  <  4:466218/137‑1 (MQ=255)
                                                                                          aTCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATa                                                                                                                   <  3:1339088/87‑1 (MQ=255)
                                                                                          aTCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATa                                                                                                                   >  4:1339088/1‑87 (MQ=255)
                                                                                          aTCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACgg                                                                    >  2:341862/1‑134 (MQ=255)
                                                                                            cGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCg                                                   <  3:1211938/149‑1 (MQ=255)
                                                                                            cGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGCTGCCGAACGGCAGTTCCCACAGTCCCg                                                   <  1:1072344/149‑1 (MQ=255)
                                                                                                  aTCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAtttt                                             >  1:1421147/1‑149 (MQ=255)
                                                                                                     aaGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGa                                                                                                >  3:1355591/1‑95 (MQ=255)
                                                                                                     aaGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGa                                                                                                <  4:1355591/95‑1 (MQ=255)
                                                                                                     aaGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCa                                          <  2:149286/149‑1 (MQ=255)
                                                                                                       ggTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCa                                                                                                                                 <  2:1083301/60‑1 (MQ=255)
                                                                                                       ggTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCa                                                                                                                                 >  1:1083301/1‑60 (MQ=255)
                                                                                                           cgcTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGAc                                    <  3:734759/149‑1 (MQ=255)
                                                                                                             cTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCgg                                                  >  2:378595/1‑133 (MQ=255)
                                                                                                             cTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCgg                                                  <  1:378595/133‑1 (MQ=255)
                                                                                                             cTTCGGTCAGGAAGTCAGTCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGa                                                                                                <  3:986246/86‑1 (MQ=255)
                                                                                                             cTTCGGTCAGGAAGTCAGTCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGa                                                                                                >  4:986246/1‑86 (MQ=255)
                                                                                                              ttCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCgg                                 >  4:1179792/1‑149 (MQ=255)
                                                                                                                 ggTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGgaga                              <  4:821320/149‑1 (MQ=255)
                                                                                                                  gTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAtt                                               <  4:1139690/131‑1 (MQ=255)
                                                                                                                  gTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAtt                                               >  3:1139690/1‑131 (MQ=255)
                                                                                                                  gTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGaa                             <  2:1421147/149‑1 (MQ=255)
                                                                                                                   tCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTccc                                                            >  1:1371004/1‑117 (MQ=255)
                                                                                                                   tCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTccc                                                            <  2:1371004/117‑1 (MQ=255)
                                                                                                                     aGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCa                                          >  1:902923/1‑133 (MQ=255)
                                                                                                                     aGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCa                                          <  2:902923/133‑1 (MQ=255)
                                                                                                                           tcagtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACg                                                                     >  2:389400/1‑100 (MQ=255)
                                                                                                                             agtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCt                  >  1:699558/1‑149 (MQ=255)
                                                                                                                               tcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGTATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGc                           >  2:1240442/1‑138 (MQ=255)
                                                                                                                               tcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGc                           <  1:1240442/138‑1 (MQ=255)
                                                                                                                                 cccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTc                                           >  4:427059/1‑120 (MQ=255)
                                                                                                                                 cccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAg                            <  3:894157/135‑1 (MQ=255)
                                                                                                                                 cccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAg                            >  4:894157/1‑135 (MQ=255)
                                                                                                                                  ccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCa                     >  2:47598/1‑141 (MQ=255)
                                                                                                                                  ccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCa                     <  1:47598/141‑1 (MQ=255)
                                                                                                                                   cgcgCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGc            >  4:58229/1‑149 (MQ=255)
                                                                                                                                    gcgcCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTa                                                                                                        >  4:185918/1‑56 (MQ=255)
                                                                                                                                     taaagccTGGCAGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGTCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGga                                <  2:1382365/123‑1 (MQ=255)
                                                                                                                                      aaagccTGGCAGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGga                                <  1:631775/123‑1 (MQ=255)
                                                                                                                                         gccTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCAt      <  3:1179792/149‑1 (MQ=255)
                                                                                                                                          ccTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATc     >  3:385680/1‑149 (MQ=255)
                                                                                                                                            tGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCtt   >  3:150985/1‑149 (MQ=255)
                                                                                                                                             ggCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCttt  >  3:296273/1‑149 (MQ=255)
                                                                                                                                             ggCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCttt  >  1:1312064/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCTTT  >  NC_000913/2400540‑2400829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: