Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,420,183 A→G 6.8% R101R (CGT→CGC yfcF ← glutathione S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,420,1830AG 6.8% 392.1 / 13.1 118R101R (CGT→CGCyfcFglutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base A (56/54);  new base G (4/4);  total (60/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

Only 100 of 119 total aligned reads displayed.

CCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAAC  >  NC_000913/2420041‑2420331
                                                                                                                                              |                                                                                                                                                    
ccATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCa                                                                                                                                                >  2:1388545/1‑149 (MQ=255)
   tCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGa                                                                                                                                             >  1:701720/1‑149 (MQ=255)
     gcgAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGAtt                                                                                                                                           >  4:1053242/1‑149 (MQ=255)
       gAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGAtttt                                                                                                                                         <  3:1053242/149‑1 (MQ=255)
       gAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGAtttt                                                                                                                                         <  2:701720/149‑1 (MQ=255)
        aaCAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTc                                                                                                                                        >  3:1318272/1‑149 (MQ=255)
        aaCAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTc                                                                                                                                        <  3:984360/149‑1 (MQ=255)
             tttCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCTCGATTTTCTAAAt                                                                                                                                   <  3:121838/149‑1 (MQ=255)
             tttCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAAt                                                                                                                                   <  4:1318272/149‑1 (MQ=255)
                 tctGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAg                                                                                                                               <  1:666710/149‑1 (MQ=255)
                    gCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCgg                                                                                                                            >  2:967763/1‑149 (MQ=255)
                        tGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATaa                                                                                                                        >  3:813595/1‑149 (MQ=255)
                            ttttCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATAc                                                                                                                    >  1:1016560/1‑149 (MQ=255)
                            ttttCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATAc                                                                                                                    <  1:1388545/149‑1 (MQ=255)
                                cccTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCaa                                                                                                                                >  1:842593/1‑133 (MQ=255)
                                 ccTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTc                                                                                                                                        <  2:953023/124‑1 (MQ=255)
                                 ccTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTc                                                                                                                                        >  1:953023/1‑124 (MQ=255)
                                        cGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCg                                                                                                        <  2:666050/149‑1 (MQ=255)
                                         gTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCgg                                                                                                       <  4:416642/149‑1 (MQ=255)
                                                gcgcTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTccc                                                                                                              <  3:1290353/135‑1 (MQ=255)
                                                gcgcTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTccc                                                                                                              >  4:1290353/1‑135 (MQ=255)
                                                gcgcTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGACGTGCACGCGCACGATTTTc                                                                                                                                        <  4:39835/109‑1 (MQ=255)
                                                gcgcTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGACGTGCACGCGCACGATTTTc                                                                                                                                        >  3:39835/1‑109 (MQ=255)
                                                   cTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTg                                                                                                     >  3:1041237/1‑141 (MQ=255)
                                                   cTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTg                                                                                                     <  4:1041237/141‑1 (MQ=255)
                                                     tttttCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGAtt                                                                                                                                           >  2:1382910/1‑101 (MQ=255)
                                                     tttttCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGAtt                                                                                                                                           <  1:1382910/101‑1 (MQ=255)
                                                       tttCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGAt                                                                                         <  1:967763/149‑1 (MQ=255)
                                                        ttCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCGCGTGCACGa                                                                                                                                             <  1:584861/96‑1 (MQ=255)
                                                        ttCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCGCGTGCACGa                                                                                                                                             >  2:584861/1‑96 (MQ=255)
                                                           gCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTc                                                                                     >  1:596418/1‑149 (MQ=255)
                                                           gCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTc                                                                                     >  3:892505/1‑149 (MQ=255)
                                                             ccccGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCGCGTGCACGacaga                                                                                                                                         <  2:735369/95‑5 (MQ=255)
                                                             ccccGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCGCGTGCACGacaga                                                                                                                                         >  1:735369/1‑91 (MQ=255)
                                                             ccccGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCa                                                                                   >  2:744298/1‑149 (MQ=255)
                                                                   aaaGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCGGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGAt                                                                                         <  1:38124/137‑1 (MQ=255)
                                                                   aaaGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATaaa                                                                                                                       <  4:1074651/107‑1 (MQ=255)
                                                                   aaaGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGAt                                                                                         >  2:38124/1‑137 (MQ=255)
                                                                   aaaGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACt                                                                             >  2:90794/1‑149 (MQ=255)
                                                                    aaGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAAt                                                                                             >  1:155653/1‑132 (MQ=255)
                                                                       acaacaTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATc                                                                                        <  2:341279/134‑1 (MQ=255)
                                                                       acaacaTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATc                                                                                        >  1:341279/1‑134 (MQ=255)
                                                                        caacaTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCa                                                                        >  1:118099/1‑149 (MQ=255)
                                                                         aacaTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTc                                                                            <  2:68618/144‑1 (MQ=255)
                                                                         aacaTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCaa                                                                       >  4:624023/1‑149 (MQ=255)
                                                                         aacaTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCaa                                                                       <  4:892505/149‑1 (MQ=255)
                                                                            aTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGAt                                                                                         >  4:1311752/1‑128 (MQ=255)
                                                                            aTCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATgg                                                                    >  2:266169/1‑149 (MQ=255)
                                                                             tCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGc                                                                   <  1:744298/149‑1 (MQ=255)
                                                                              ccGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCa                                                                  <  2:118099/149‑1 (MQ=255)
                                                                               cGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCag                                                                 >  3:29260/1‑149 (MQ=255)
                                                                                gTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCaga                                                                <  4:29260/149‑1 (MQ=255)
                                                                                    gACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGAtt                                                                                                                                           >  1:517063/1‑70 (MQ=255)
                                                                                     aCGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGAtt                                                           <  1:90794/149‑1 (MQ=255)
                                                                                      cGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTc                                                          <  3:624023/149‑1 (MQ=255)
                                                                                       gCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCa                                                         >  2:803828/1‑149 (MQ=255)
                                                                                        ctctTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCac                                                        <  1:109066/149‑1 (MQ=255)
                                                                                                ggATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGAtt                                                           <  4:1121738/138‑1 (MQ=255)
                                                                                                ggATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGAtt                                                           >  3:1121738/1‑138 (MQ=255)
                                                                                                    gggCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCa                                                         >  3:427052/1‑136 (MQ=255)
                                                                                                      gCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGa                                                                                          <  3:1103222/101‑1 (MQ=255)
                                                                                                      gCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGa                                                                                          >  4:1103222/1‑101 (MQ=255)
                                                                                                       cATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTgg                                                                                                    >  3:991752/1‑90 (MQ=255)
                                                                                                       cATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATc                                                                                        <  4:854626/102‑1 (MQ=255)
                                                                                                       cATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATc                                                                                        >  3:854626/1‑102 (MQ=255)
                                                                                                       cATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCactcaac                                                   >  2:82844/1‑139 (MQ=255)
                                                                                                       cATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAatcat                                         <  3:1331552/149‑1 (MQ=255)
                                                                                                       cATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAatcat                                         <  4:716721/149‑1 (MQ=255)
                                                                                                       cATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAatcat                                         <  2:596418/149‑1 (MQ=255)
                                                                                                        aTCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCa                                                         <  1:338125/132‑1 (MQ=255)
                                                                                                        aTCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCa                                                         >  2:338125/1‑132 (MQ=255)
                                                                                                         tCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCaaaa                                             <  3:350750/143‑1 (MQ=255)
                                                                                                         tCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCaaaa                                             >  4:350750/1‑143 (MQ=255)
                                                                                                           aGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCaaaa                                             >  4:508190/1‑141 (MQ=255)
                                                                                                           aGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCaaaa                                             <  3:508190/141‑1 (MQ=255)
                                                                                                           aGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGAt                                     <  1:123275/149‑1 (MQ=255)
                                                                                                           aGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGAt                                     >  4:677129/1‑149 (MQ=255)
                                                                                                            gATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTgg                                                                                                    >  1:707384/1‑85 (MQ=255)
                                                                                                            gATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGAtt                                    >  3:889654/1‑149 (MQ=255)
                                                                                                             aTCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGAttt                                   >  2:692840/1‑149 (MQ=255)
                                                                                                               cGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGt                                 <  4:889654/149‑1 (MQ=255)
                                                                                                                gCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGta                                >  2:435921/1‑149 (MQ=255)
                                                                                                                gCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGta                                <  1:803828/149‑1 (MQ=255)
                                                                                                                     gcAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGt                                 >  1:825255/1‑143 (MQ=255)
                                                                                                                     gcAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGt                                 <  2:825255/143‑1 (MQ=255)
                                                                                                                       aGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACgg                         >  1:997595/1‑149 (MQ=255)
                                                                                                                        gCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGc                        <  3:677129/149‑1 (MQ=255)
                                                                                                                         ccAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGtaataa                            >  3:596233/1‑144 (MQ=255)
                                                                                                                         ccAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGtaataa                            <  4:596233/144‑1 (MQ=255)
                                                                                                                            ggCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCacac                    <  1:266169/149‑1 (MQ=255)
                                                                                                                              ccTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGt                                                                                                      <  2:149561/65‑1 (MQ=255)
                                                                                                                              ccTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGt                                                                                                      >  1:149561/1‑65 (MQ=255)
                                                                                                                               cTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTTCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGc                 <  4:899319/149‑1 (MQ=255)
                                                                                                                               cTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGc                 >  4:1102712/1‑149 (MQ=255)
                                                                                                                                      aatCGTGCGCGTGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCa                                                         >  4:838100/3‑102 (MQ=255)
                                                                                                                                      aatCGTGCGCGTGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCa                                                         <  3:838100/100‑1 (MQ=255)
                                                                                                                                      aatCGTGCGCGTGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGtaataa                            <  4:207255/129‑1 (MQ=255)
                                                                                                                                      aatCGTGCGCGTGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGtaataa                            >  3:207255/3‑131 (MQ=255)
                                                                                                                                        tCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGAGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGAc        >  2:1189856/1‑149 (MQ=255)
                                                                                                                                              aCGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTCACCGTAAc  <  3:1102712/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTTCCAGATACTCCGCAATGGCAGAAGATTCACTCAACTCAAAATCATCGATTTGTAATAACGGCACACGGCGTGTCTGACCGTAAC  >  NC_000913/2420041‑2420331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: