Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,432,985 A→G 7.9% intergenic (‑43/+27) folC ← / ← accD bifunctional folylpolyglutamate synthase/ dihydrofolate synthase/acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,432,9850AG 7.9% 247.3 / 8.6 76intergenic (‑43/+27)folC/accDbifunctional folylpolyglutamate synthase/ dihydrofolate synthase/acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
Reads supporting (aligned to +/- strand):  ref base A (34/36);  new base G (3/3);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

TCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATT  >  NC_000913/2432837‑2433132
                                                                                                                                                    |                                                                                                                                                   
tCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCaga                                                                                                                                                     <  2:50984/149‑1 (MQ=255)
        tCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTgg                                                                                                                                             <  4:30049/149‑1 (MQ=255)
        tCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTgg                                                                                                                                             >  3:30049/1‑149 (MQ=255)
         cAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgc                                                                                                                                            <  2:209316/149‑1 (MQ=255)
            ggCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccct                                                                                                                                         <  3:1175975/149‑1 (MQ=255)
                   aTCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTg                                                                                                                                              >  3:1008883/1‑137 (MQ=255)
                   aTCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTg                                                                                                                                              <  4:1008883/137‑1 (MQ=255)
                      gATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccct                                                                                                                                         >  1:1292278/1‑139 (MQ=255)
                      gATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccct                                                                                                                                         <  2:1292278/139‑1 (MQ=255)
                      gATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                               >  2:950657/1‑149 (MQ=255)
                         aGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagtt                                                                                                                            <  3:238282/149‑1 (MQ=255)
                           ttttACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTGGCTCTGCCCtttt                                                                                                                                 <  3:1233913/142‑3 (MQ=255)
                           ttttACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTGGCTCTGCCCtttt                                                                                                                                 >  4:1233913/1‑140 (MQ=255)
                                    gCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccctgc                                                                                                                                       >  3:1219332/1‑127 (MQ=255)
                                    gCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccctgc                                                                                                                                       <  4:1219332/127‑1 (MQ=255)
                                     cAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCa                                                                                                                 <  2:577283/148‑1 (MQ=255)
                                     cAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCa                                                                                                                 >  1:577283/1‑148 (MQ=255)
                                      aGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                               >  2:554406/1‑133 (MQ=255)
                                      aGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                               >  2:554821/1‑133 (MQ=255)
                                      aGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                               <  1:554821/133‑1 (MQ=255)
                                      aGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                               <  1:554406/133‑1 (MQ=255)
                                               aGATAAGAAAGGCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATc                                                                                                      >  3:837611/1‑149 (MQ=255)
                                               aGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATc                                                                                                      >  4:96720/1‑149 (MQ=255)
                                                      aaaGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTGgctctgccc                                                                                                                                     >  3:1445799/1‑111 (MQ=255)
                                                      aaaGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTGgctctgccc                                                                                                                                     <  4:1445799/111‑1 (MQ=255)
                                                          ccACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCgggg                                                                                           <  1:950657/149‑1 (MQ=255)
                                                           cACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCa                                                                                                                 <  1:1112854/126‑1 (MQ=255)
                                                           cACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCa                                                                                                                 >  2:1112854/1‑126 (MQ=255)
                                                            aCGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCgg                                                                                             >  1:230313/1‑145 (MQ=255)
                                                            aCGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCgg                                                                                             <  2:230313/145‑1 (MQ=255)
                                                              gAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGa                                                                                                        >  3:680372/1‑132 (MQ=255)
                                                              gAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGa                                                                                                        <  4:680372/132‑1 (MQ=255)
                                                              gAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTAc                                                                                       <  2:957513/149‑1 (MQ=255)
                                                              gAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTAc                                                                                       <  4:837611/149‑1 (MQ=255)
                                                                aGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCa                                                                                     >  2:742123/1‑149 (MQ=255)
                                                                         cttcgtggCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCtt                                                                            <  1:964132/143‑1 (MQ=255)
                                                                             gcgGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCg                                                                                              >  2:1136759/1‑127 (MQ=255)
                                                                             gcgGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCg                                                                                              <  1:1136759/127‑1 (MQ=255)
                                                                                 cTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACgcggc                                                                    <  1:742123/149‑1 (MQ=255)
                                                                                  ttGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACgcggcg                                                                   >  3:583489/1‑149 (MQ=255)
                                                                                    gaggagTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCgg                                                                                             <  4:1075356/121‑1 (MQ=255)
                                                                                    gaggagTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCgg                                                                                             >  3:1075356/1‑121 (MQ=255)
                                                                                             gTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTcc                                                                                                           <  4:1180513/98‑1 (MQ=255)
                                                                                             gTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTcc                                                                                                           >  3:1180513/1‑98 (MQ=255)
                                                                                              tttGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTg                                                                                                                                              <  2:1012191/62‑1 (MQ=255)
                                                                                              tttGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTg                                                                                                                                              >  1:1012191/1‑62 (MQ=255)
                                                                                                 gATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATc                                                                                                      <  2:65754/99‑1 (MQ=255)
                                                                                                 gATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATc                                                                                                      >  1:65754/1‑99 (MQ=255)
                                                                                                 gATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCgggg                                                                                           >  3:658591/1‑110 (MQ=255)
                                                                                                 gATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCgggg                                                                                           <  4:658591/110‑1 (MQ=255)
                                                                                                     aTCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccctgc                                                                                                                                       >  1:184970/1‑62 (MQ=255)
                                                                                                     aTCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccctgc                                                                                                                                       <  2:184970/62‑1 (MQ=255)
                                                                                                     aTCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTgg                                                >  3:1437039/1‑149 (MQ=255)
                                                                                                      tCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagttatca                                                                                                                        >  3:201761/1‑76 (MQ=255)
                                                                                                      tCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagttatca                                                                                                                        <  4:201761/76‑1 (MQ=255)
                                                                                                      tCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCa                                                              >  1:386575/1‑134 (MQ=255)
                                                                                                      tCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCa                                                              <  2:386575/134‑1 (MQ=255)
                                                                                                              tCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtcat                                       >  1:59755/1‑149 (MQ=255)
                                                                                                                 gCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAg                                                                                                                <  3:804087/73‑1 (MQ=255)
                                                                                                                 gCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAg                                                                                                                >  4:804087/1‑73 (MQ=255)
                                                                                                                 gCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTcc                                                                                                           >  1:186270/1‑78 (MQ=255)
                                                                                                                 gCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTAAGGTTcc                                                                                                           <  2:186270/78‑1 (MQ=255)
                                                                                                                               gTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGc                      >  3:422084/1‑149 (MQ=255)
                                                                                                                                          aaaaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTcc                                                                                                           <  2:711609/53‑1 (MQ=255)
                                                                                                                                          aaaaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTcc                                                                                                           >  1:711609/1‑53 (MQ=255)
                                                                                                                                          aaaaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCtt                                                                            <  2:537613/84‑1 (MQ=255)
                                                                                                                                          aaaaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCtt                                                                            >  1:537613/1‑84 (MQ=255)
                                                                                                                                           aaaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCa          <  4:422084/149‑1 (MQ=255)
                                                                                                                                            aaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg         <  2:59755/149‑1 (MQ=255)
                                                                                                                                               ggCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtc                                         <  2:189372/114‑1 (MQ=255)
                                                                                                                                               ggCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtc                                         >  1:189372/1‑114 (MQ=255)
                                                                                                                                                gCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCtt                                                                            >  1:233934/1‑78 (MQ=255)
                                                                                                                                                gCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCtt                                                                            <  2:233934/78‑1 (MQ=255)
                                                                                                                                                gCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTc                    <  4:179458/134‑1 (MQ=255)
                                                                                                                                                gCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTc                    >  3:179458/1‑134 (MQ=255)
                                                                                                                                                gCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCa          >  4:1370235/1‑144 (MQ=255)
                                                                                                                                                gCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCa          <  3:1370235/144‑1 (MQ=255)
                                                                                                                                                  agagCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAg                                                             <  1:525636/91‑1 (MQ=255)
                                                                                                                                                  agagCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAg                                                             >  2:525636/1‑91 (MQ=255)
                                                                                                                                                   gagCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCAtt  <  4:658924/149‑1 (MQ=255)
                                                                                                                                                   gagCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCAtt  >  2:229726/1‑149 (MQ=255)
                                                                                                                                                   gagCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCAtt  >  3:658924/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATT  >  NC_000913/2432837‑2433132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: