Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,532,279 G→A 14.7% intergenic (+55/‑130) cysZ → / → cysK sulfate transporter, sulfite inhibited/cysteine synthase A, O‑acetylserine sulfhydrolase A subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,532,2790GA14.7% 175.4 / 21.9 68intergenic (+55/‑130)cysZ/cysKsulfate transporter, sulfite inhibited/cysteine synthase A, O‑acetylserine sulfhydrolase A subunit
Reads supporting (aligned to +/- strand):  ref base G (30/28);  new base A (4/6);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

GCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTT  >  NC_000913/2532134‑2532421
                                                                                                                                                 |                                                                                                                                              
gctTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGCGGCATAAGCCAccc                                                                                                                                             <  2:231267/149‑1 (MQ=255)
     aTCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTAt                                                                                                                                        >  4:1029588/1‑149 (MQ=255)
       cTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTc                                                                                                                                      <  3:1029588/149‑1 (MQ=255)
        tGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGCGGCATAAGCCACCCCtgtttca                                                                                                                                     <  4:1227283/149‑7 (MQ=255)
          ttCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCAt                                                                                                                                   >  1:1313687/1‑149 (MQ=255)
             atcatGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCtt                                                                                                                                >  1:1321413/1‑149 (MQ=255)
                       ttGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCatt                                                                                                                      >  1:1135276/1‑149 (MQ=255)
                       ttGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGCTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCatt                                                                                                                      <  2:1135276/149‑1 (MQ=255)
                           cGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGcccc                                                                                                                                            >  2:949553/1‑123 (MQ=255)
                           cGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTAttt                                                                                                                  <  4:60412/149‑1 (MQ=255)
                           cGTTAGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGcccc                                                                                                                                            <  1:949553/123‑1 (MQ=255)
                            gTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTAt                                                                                                                                        <  1:930038/126‑1 (MQ=255)
                            gTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTAt                                                                                                                                        >  2:930038/1‑126 (MQ=255)
                                gtgGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCtt                                                                                                             >  1:290881/1‑149 (MQ=255)
                                   gcgcGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCt                                                                                                           >  1:519424/1‑148 (MQ=255)
                                   gcgcGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCt                                                                                                           <  2:519424/148‑1 (MQ=255)
                                     gcgACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGta                                                                                                        >  1:1291108/1‑149 (MQ=255)
                                                gtgGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCt                                                                                                           <  1:374426/135‑1 (MQ=255)
                                                gtgGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCt                                                                                                           >  2:374426/1‑135 (MQ=255)
                                                      cGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATc                                                                                       <  2:1291108/149‑1 (MQ=255)
                                                        aTTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGCGGCATAAGCCAccc                                                                                                                                             >  4:267384/1‑93 (MQ=255)
                                                        aTTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGCGGCATAAGCCAccc                                                                                                                                             <  3:267384/93‑1 (MQ=255)
                                                         ttGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCt                                                                                     >  4:1121684/1‑148 (MQ=255)
                                                         ttGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCt                                                                                     <  3:1121684/148‑1 (MQ=255)
                                                             tATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTg                                                                                                          >  2:679550/1‑123 (MQ=255)
                                                             tATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTg                                                                                                          <  1:679550/123‑1 (MQ=255)
                                                             tATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACtt                                                                                >  2:67313/1‑149 (MQ=255)
                                                               tCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTcc                                                                              >  3:509707/1‑149 (MQ=255)
                                                                  cgATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgccgcc                                                                                                                                              >  3:91647/1‑82 (MQ=255)
                                                                  cgATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTCAAACAGGGGTGGCTTATgccgcc                                                                                                                                              <  4:91647/82‑1 (MQ=255)
                                                                   gATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTcc                                                                                                                <  1:325992/111‑1 (MQ=255)
                                                                   gATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTcc                                                                                                                >  2:325992/1‑111 (MQ=255)
                                                                      aaaCACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTa                                                                                   >  3:703884/1‑137 (MQ=255)
                                                                      aaaCACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTa                                                                                   <  4:703884/137‑1 (MQ=255)
                                                                       aaCACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATtc                                                                      <  2:1321413/149‑1 (MQ=255)
                                                                         cacGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATtctc                                                                    <  2:290881/149‑1 (MQ=255)
                                                                         cacGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATtctc                                                                    <  4:509707/149‑1 (MQ=255)
                                                                                    gCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTAt                                                         >  1:1225476/1‑149 (MQ=255)
                                                                                    gCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGAGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCt                                                                                                              >  3:1408675/1‑96 (MQ=255)
                                                                                    gCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGAGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCt                                                                                                              <  4:1408675/96‑1 (MQ=255)
                                                                                          aCAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTAcc                                                   <  2:1225476/149‑1 (MQ=255)
                                                                                            aaTCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCtg                                                 >  2:540626/1‑149 (MQ=255)
                                                                                             aTCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCtgt                                                >  2:732549/1‑149 (MQ=255)
                                                                                                tACCGGTTATTTTCTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCtgttgt                                             >  3:78913/1‑149 (MQ=255)
                                                                                                  ccGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTAc                                                                                  >  2:122804/1‑110 (MQ=255)
                                                                                                  ccGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTAc                                                                                  <  1:122804/110‑1 (MQ=255)
                                                                                                   cGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATc                                          >  1:983628/1‑149 (MQ=255)
                                                                                                            tGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCa                                 <  2:1313687/149‑1 (MQ=255)
                                                                                                              tcaaCCGTTTGTGTGAAACAGGGGTAGCTTTTGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCCGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGCTATGCTACCTGTTGTATCCCAATTTCATa                               <  4:78913/147‑1 (MQ=255)
                                                                                                              tAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATa                               <  2:983628/149‑1 (MQ=255)
                                                                                                                aaCCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACa                             >  4:508696/1‑149 (MQ=255)
                                                                                                                aaCCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACa                             >  1:1075523/1‑149 (MQ=255)
                                                                                                                 aCCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCtt                                                                                    <  2:227390/93‑1 (MQ=255)
                                                                                                                 aCCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCtt                                                                                    >  1:227390/1‑93 (MQ=255)
                                                                                                                      ttGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAg                       <  1:732549/149‑1 (MQ=255)
                                                                                                                        gtgtgAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGa                     <  1:540626/149‑1 (MQ=255)
                                                                                                                          gtgAATCAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTa                                                                                   <  1:1174502/85‑1 (MQ=255)
                                                                                                                          gtgAATCAGGGGTGGCTTATGCCGCCCCTTATTCCATATTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTa                                                                                   >  2:1174502/1‑85 (MQ=255)
                                                                                                                              ataaGGGGCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGc                                                               >  4:892523/4‑101 (MQ=255)
                                                                                                                              ataaGGGGCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGc                                                               <  3:892523/98‑1 (MQ=255)
                                                                                                                               taaGGGGCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCgg                                                             <  3:528573/100‑1 (MQ=255)
                                                                                                                               taaGGGGCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCgg                                                             >  4:528573/3‑102 (MQ=255)
                                                                                                                                  ggggCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAAt                                     <  1:652947/123‑1 (MQ=255)
                                                                                                                                  ggggCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAAt                                     >  2:652947/1‑123 (MQ=255)
                                                                                                                                   gggTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACa                             >  3:1457305/1‑130 (MQ=255)
                                                                                                                                   gggTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACa                             <  4:1457305/130‑1 (MQ=255)
                                                                                                                                      tGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTaa       <  2:1075523/149‑1 (MQ=255)
                                                                                                                                         cTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGtatata                                                                                                    >  1:981941/1‑53 (MQ=255)
                                                                                                                                         cTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGtatata                                                                                                    <  2:981941/53‑1 (MQ=255)
                                                                                                                                          ttATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGAtt   >  4:214653/1‑149 (MQ=255)
                                                                                                                                               ccgccCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGAttt  >  2:561053/1‑145 (MQ=255)
                                                                                                                                               ccgccCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGAttt  <  1:561053/145‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTT  >  NC_000913/2532134‑2532421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: