| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,549,609 | G→A | 5.1% | intergenic (+203/+37) | yfeW → / ← yfeX | penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity/porphyrinogen oxidase, cytoplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,549,609 | 0 | G | A | 5.1% | 336.0 / 5.8 | 98 | intergenic (+203/+37) | yfeW/yfeX | penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity/porphyrinogen oxidase, cytoplasmic |
| Reads supporting (aligned to +/- strand): ref base G (51/42); new base A (3/2); total (54/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01 | |||||||||||
GCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTC > NC_000913/2549475‑2549757 | gcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGtt > 1:833123/1‑137 (MQ=255)gcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGtt < 2:833123/137‑1 (MQ=255)gcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCt > 3:1113315/1‑149 (MQ=255) cTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCtt > 2:860136/1‑149 (MQ=255) gCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGTGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAAt > 2:419645/1‑149 (MQ=255) cTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATc < 4:1113315/149‑1 (MQ=255) tCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTAACATTACTgac < 1:1280188/133‑3 (MQ=37) tCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTAACATTACTgac > 2:1280188/1‑131 (MQ=25) aGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCt > 1:130611/1‑137 (MQ=255) aGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCt < 2:130611/137‑1 (MQ=255) gTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCtt > 2:199904/1‑130 (MQ=255) gTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCtt < 1:199904/130‑1 (MQ=255) ggAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGc < 1:860136/149‑1 (MQ=255) ccACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACa < 1:419645/149‑1 (MQ=255) cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAAt > 2:445089/1‑126 (MQ=255) cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAAt < 1:445089/126‑1 (MQ=255) acaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAgc > 1:1177753/1‑149 (MQ=255) tGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAAc < 2:1177753/149‑1 (MQ=255) gCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATc < 2:536644/110‑1 (MQ=255) gCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATc > 1:536644/1‑110 (MQ=255) aCGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACt > 3:994038/1‑93 (MQ=255) aCGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACt < 4:994038/93‑1 (MQ=255) gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTa > 1:1138440/1‑119 (MQ=255) gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTa < 2:1138440/119‑1 (MQ=255) aGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTc < 1:146552/135‑1 (MQ=255) aGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTc > 2:146552/1‑135 (MQ=255) gCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGc < 3:916383/112‑1 (MQ=255) gCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGc > 4:916383/1‑112 (MQ=255) cGGATGCGGCGTGAACGCCTTATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTgg > 1:824892/1‑85 (MQ=37) cGGATGCGGCGTGAACGCCTTATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTgg < 2:824892/85‑1 (MQ=37) ttCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATc < 4:1361128/112‑1 (MQ=255) ttCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATc > 3:1361128/1‑112 (MQ=255) cACGCCGGATGCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAAc < 3:506078/95‑1 (MQ=255) cACGCCGGATGCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAAc > 4:506078/1‑95 (MQ=255) aCGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAAt < 3:633619/83‑1 (MQ=255) aCGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAAt > 4:633619/1‑83 (MQ=255) aCGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTa > 1:1263605/1‑149 (MQ=255) ccgcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGt > 3:541110/1‑58 (MQ=255) ccgcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGt < 4:541110/58‑1 (MQ=255) ccgcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCg > 4:1300345/1‑149 (MQ=255) cgcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCaa > 1:614600/1‑78 (MQ=255) cgcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCaa < 2:614600/78‑1 (MQ=255) tCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCt < 3:1281166/61‑1 (MQ=255) tCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCt > 4:1281166/1‑61 (MQ=255) gCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAAt < 1:512870/71‑1 (MQ=255) gCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAAt > 2:512870/1‑71 (MQ=255) gCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGa > 1:149737/1‑149 (MQ=255) tACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGtt < 1:163871/138‑1 (MQ=255) tACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGtt > 2:163871/1‑138 (MQ=255) aCAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGcc < 3:1258016/88‑1 (MQ=255) aCAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGcc > 4:1258016/1‑88 (MQ=255) aaCGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCgg < 2:914370/126‑1 (MQ=255) aaCGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCgg > 1:914370/1‑126 (MQ=255) aCGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACtt < 1:380820/93‑1 (MQ=255) aCGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACtt > 2:380820/1‑93 (MQ=255) cACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCa < 2:149737/149‑1 (MQ=255) tCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCt > 1:203943/1‑64 (MQ=255) tCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCt < 2:203943/64‑1 (MQ=255) aacaaTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCaa > 3:290383/1‑133 (MQ=255) aacaaTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCaa < 4:971729/133‑1 (MQ=255) aacaaTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCaa > 3:971729/1‑133 (MQ=255) aacaaTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCaa < 4:290383/133‑1 (MQ=255) aacaaTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTa > 1:547580/1‑149 (MQ=255) acaaTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGtt > 2:1350141/1‑119 (MQ=255) acaaTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGtt < 1:1350141/119‑1 (MQ=255) aTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCAt < 2:547580/149‑1 (MQ=255) tCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATc < 3:1300345/149‑1 (MQ=255) ggCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAAc > 1:1266515/1‑119 (MQ=255) ggCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAAc < 2:1266515/119‑1 (MQ=255) gCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAataata < 2:30730/92‑1 (MQ=255) gCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAataata > 1:30730/1‑92 (MQ=255) gCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCa < 1:52080/129‑1 (MQ=255) gCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCa > 2:52080/1‑129 (MQ=255) gCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATc > 4:1299169/1‑149 (MQ=255) ccAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCg > 1:475230/1‑149 (MQ=255) caggcagtAACATTACTGACTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCgt > 3:126138/1‑148 (MQ=255) aggcagtAACATTACTGACTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGcc > 2:1296891/4‑149 (MQ=255) gtcagtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCgg < 2:63429/141‑1 (MQ=255) gtcagtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCgg > 1:63429/1‑141 (MQ=255) gtcagtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCa > 3:862191/1‑149 (MQ=255) tcggtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGc < 4:1346833/127‑1 (MQ=255) tcggtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGc > 3:1346833/4‑130 (MQ=255) tcagtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCaa > 2:76569/1‑149 (MQ=255) cagtAACATTACTGACTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCaaa < 1:1296891/149‑1 (MQ=255) agtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAgc > 4:1217283/1‑87 (MQ=255) agtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAgc < 3:1217283/87‑1 (MQ=255) agtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCa > 4:1192192/1‑125 (MQ=255) agtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCa < 3:1192192/125‑1 (MQ=255) agtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAAc > 3:262012/1‑149 (MQ=255) gtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTg < 1:64017/74‑1 (MQ=255) gtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTg > 2:64017/1‑74 (MQ=255) gtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAaataa < 1:1371947/82‑1 (MQ=255) gtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAaataa > 2:1371947/1‑82 (MQ=255) gtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACa > 2:267972/1‑149 (MQ=255) gtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACa > 4:1625/1‑149 (MQ=255) aTGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGc > 2:1041425/1‑149 (MQ=255) tGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGt < 2:965061/69‑1 (MQ=255) tGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGt > 1:965061/1‑69 (MQ=255) gTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTc > 2:1165203/1‑149 (MQ=255) | GCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTC > NC_000913/2549475‑2549757 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |