Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,550,578 C→G 10.0% intergenic (‑33/+63) yfeX ← / ← yfeY porphyrinogen oxidase, cytoplasmic/RpoE‑regulated lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,550,5780CG10.0% 201.0 / 13.2 60intergenic (‑33/+63)yfeX/yfeYporphyrinogen oxidase, cytoplasmic/RpoE‑regulated lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (27/27);  new base G (3/3);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-02

GGCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCA  >  NC_000913/2550432‑2550716
                                                                                                                                                  |                                                                                                                                          
ggCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCg                                                                                                                                          >  3:334710/1‑149 (MQ=255)
 gCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGt                                                                                                                                         <  3:289332/149‑1 (MQ=255)
   aaaTGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCTGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTATTCCTCCTGAAAATAATAATGCCGCGCAATCCGTgg                                                                                                                                       <  4:1238501/149‑1 (MQ=255)
   aaaTGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTgg                                                                                                                                       <  4:334710/149‑1 (MQ=255)
              tGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTgg                                                                                                                                       >  1:1412865/1‑138 (MQ=255)
              tGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTgg                                                                                                                                       <  2:1412865/138‑1 (MQ=255)
                  cgcacgcaGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCAttgtt                                                                                                                                >  4:1372515/1‑141 (MQ=255)
                  cgcacgcaGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGAAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCAttgtt                                                                                                                                <  3:1372515/141‑1 (MQ=255)
                                    ttCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAg                                                                                                                             <  3:1244095/126‑1 (MQ=255)
                                    ttCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAg                                                                                                                             >  4:1244095/1‑126 (MQ=255)
                                    ttCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGttt                                                                                                            <  3:1221081/143‑1 (MQ=255)
                                    ttCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGttt                                                                                                            >  4:1221081/1‑143 (MQ=255)
                                          tttCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCgcggcggc                                                                                                >  1:275741/1‑149 (MQ=255)
                                                gTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGa                                                                                                                              >  3:243775/1‑113 (MQ=255)
                                                gTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGa                                                                                                                              <  4:243775/113‑1 (MQ=255)
                                                  tGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGtt                                                                                        <  2:275741/149‑1 (MQ=255)
                                                      ttCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTAc                                                                                                                  <  3:130017/119‑1 (MQ=255)
                                                      ttCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTAc                                                                                                                  >  4:130017/1‑119 (MQ=255)
                                                                 tCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAAc                                                                         >  4:177536/1‑149 (MQ=255)
                                                                             aaTGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAgcg                                                                                                                           <  3:1329607/87‑1 (MQ=255)
                                                                             aaTGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAgcg                                                                                                                           >  4:1329607/1‑87 (MQ=255)
                                                                                  tCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTa                                                        <  3:177536/149‑1 (MQ=255)
                                                                                    tGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTAc                                                       <  2:975506/148‑1 (MQ=255)
                                                                                    tGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTAc                                                       >  1:975506/1‑148 (MQ=255)
                                                                                      gCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAAc                                                                         <  2:132049/128‑1 (MQ=255)
                                                                                      gCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAAc                                                                         >  1:132049/1‑128 (MQ=255)
                                                                                      gCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGa                                                    >  3:248312/1‑149 (MQ=255)
                                                                                        aaaaTGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGtt                                                                                        <  3:1277854/111‑1 (MQ=255)
                                                                                        aaaaTGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGtt                                                                                        >  4:1277854/1‑111 (MQ=255)
                                                                                        aaaaTGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACt                                                  >  2:192113/1‑149 (MQ=255)
                                                                                          aaTGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGttt                                                                                           >  4:138810/1‑106 (MQ=255)
                                                                                          aaTGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGttt                                                                                           <  3:138810/106‑1 (MQ=255)
                                                                                           aTGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTc                                               <  1:192113/149‑1 (MQ=255)
                                                                                               cACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGtt                                                                                        >  4:1349557/1‑104 (MQ=255)
                                                                                               cACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGtt                                                                                        <  3:1349557/104‑1 (MQ=255)
                                                                                                aCTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGtt                                          <  4:248312/149‑1 (MQ=255)
                                                                                                  tctGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTc                                               <  3:389577/142‑1 (MQ=255)
                                                                                                  tctGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTc                                               >  4:389577/1‑142 (MQ=255)
                                                                                                         cTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTc                                                                                                        <  3:444304/78‑1 (MQ=255)
                                                                                                         cTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTc                                                                                                        >  4:444304/1‑78 (MQ=255)
                                                                                                                 ttGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGtttttt                                                                                                         <  2:1438641/69‑1 (MQ=255)
                                                                                                                 ttGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGtttttt                                                                                                         >  1:1438641/1‑69 (MQ=255)
                                                                                                                          ctGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTa                >  4:179492/1‑149 (MQ=255)
                                                                                                                               aataataatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGtt                                                                                        <  2:880911/72‑1 (MQ=255)
                                                                                                                               aataataatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGtt                                                                                        >  1:880911/1‑72 (MQ=255)
                                                                                                                               aataataatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCt           >  1:970328/1‑149 (MQ=255)
                                                                                                                                tcaacaatGCCACGGATTGCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACt                                                      >  3:155142/6‑105 (MQ=38)
                                                                                                                                tcaacaatGCCACGGATTGCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACt                                                      <  4:155142/100‑1 (MQ=38)
                                                                                                                                ataataatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTcgc                                                                                                      >  1:1210601/1‑57 (MQ=255)
                                                                                                                                ataataatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTcgc                                                                                                      <  2:1210601/57‑1 (MQ=255)
                                                                                                                                   acaatGCCACGGATTGCGTGGCATTATTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCa                                     >  3:994817/3‑119 (MQ=255)
                                                                                                                                   acaatGCCACGGATTGCGTGGCATTATTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCa                                     <  4:994817/117‑1 (MQ=255)
                                                                                                                                     aatGCCACGGATTGCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAgg                                   >  1:1163439/1‑119 (MQ=255)
                                                                                                                                     aatGCCACGGATTGCGTGGCAGTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAgg                                   <  2:1163439/119‑1 (MQ=255)
                                                                                                                                        gCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCa  >  3:529893/1‑149 (MQ=255)
                                                                                                                                        gCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCa  <  3:179492/149‑1 (MQ=255)
                                                                                                                                         ccACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCa                                             >  2:1224699/1‑105 (MQ=255)
                                                                                                                                         ccACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCa                                             <  1:1224699/105‑1 (MQ=255)
                                                                                                                                           aCGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGAc                                                                                   <  4:107470/65‑1 (MQ=255)
                                                                                                                                           aCGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGAc                                                                                   >  3:107470/1‑65 (MQ=255)
                                                                                                                                                  |                                                                                                                                          
GGCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCA  >  NC_000913/2550432‑2550716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑