Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,581,762 G→A 6.0% A339V (GCC→GTC)  ypfG ← DUF1176 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,581,7620GA 6.0% 270.9 / 6.4 83A339V (GCC→GTC) ypfGDUF1176 family protein
Reads supporting (aligned to +/- strand):  ref base G (42/36);  new base A (3/2);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-01

GTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGAT  >  NC_000913/2581616‑2581905
                                                                                                                                                  |                                                                                                                                               
gTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCg                                                                                                                                               >  3:167925/1‑149 (MQ=255)
     gggAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTgg                                                                                                                                          <  4:167925/149‑1 (MQ=255)
                                                       gACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGc                                                                                                                                   >  3:1190259/1‑106 (MQ=255)
                                                       gACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGc                                                                                                                                   <  4:1190259/106‑1 (MQ=255)
                                                       gACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaa                                                                                        >  2:1297520/1‑149 (MQ=255)
                                                             gACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCt                                                                                  <  1:1297520/149‑1 (MQ=255)
                                                              aCGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTg                                                                                 >  3:679840/1‑149 (MQ=255)
                                                                   tCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGt                                                                            >  1:63891/1‑149 (MQ=255)
                                                                     ttATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgc                                                                                                            >  3:1445942/1‑115 (MQ=255)
                                                                     ttATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgc                                                                                                            <  4:1445942/115‑1 (MQ=255)
                                                                     ttATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCa                                                                          >  2:337636/1‑149 (MQ=255)
                                                                        tCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaaaa                                                                                      >  3:115439/1‑134 (MQ=255)
                                                                        tCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaaaa                                                                                      <  4:115439/134‑1 (MQ=255)
                                                                        tCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAg                                                                       >  2:794811/1‑149 (MQ=255)
                                                                           ggCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGt                                                                            >  1:235923/1‑141 (MQ=255)
                                                                           ggCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGt                                                                            <  2:235923/141‑1 (MQ=255)
                                                                             ccTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCa                                                                  >  3:869476/1‑149 (MQ=255)
                                                                              cTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATc                                                                                                                           >  4:179602/1‑91 (MQ=255)
                                                                              cTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATc                                                                                                                           <  3:179602/91‑1 (MQ=255)
                                                                              cTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGc                                                                                   >  1:1454916/1‑131 (MQ=255)
                                                                              cTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGc                                                                                   <  2:1454916/131‑1 (MQ=255)
                                                                                   gCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAgcgcgc                                                            <  2:1157201/149‑1 (MQ=255)
                                                                                   gCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAgcgcgc                                                            >  1:1157201/1‑149 (MQ=255)
                                                                                     gTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGACt                                                          >  1:1332104/1‑149 (MQ=255)
                                                                                        ttATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGc                                                                                   >  2:1132557/1‑121 (MQ=255)
                                                                                        ttATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGc                                                                                   <  1:1132557/121‑1 (MQ=255)
                                                                                        ttATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAgcgc                                                                    >  4:1086557/1‑136 (MQ=255)
                                                                                        ttATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAgcgc                                                                    <  3:1086557/136‑1 (MQ=255)
                                                                                         tATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAAt                                                      <  4:679840/149‑1 (MQ=255)
                                                                                          aTCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATg                                                     >  4:1049837/1‑149 (MQ=255)
                                                                                           tCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGc                                                    >  2:481022/1‑149 (MQ=255)
                                                                                              ggCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaaaa                                                                                      >  2:1230037/1‑112 (MQ=255)
                                                                                              ggCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaaaa                                                                                      <  1:1230037/112‑1 (MQ=255)
                                                                                                 cTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAAt                                              >  2:480815/1‑149 (MQ=255)
                                                                                                    cATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                           <  3:1049837/149‑1 (MQ=255)
                                                                                                     aTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCt                                          <  4:869476/149‑1 (MQ=255)
                                                                                                       gTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTa                                        >  2:1213222/1‑149 (MQ=255)
                                                                                                            gCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                           >  2:516556/1‑141 (MQ=255)
                                                                                                            gCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                           <  1:516556/141‑1 (MQ=255)
                                                                                                              cATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTgg                                                                                                                   >  4:162941/1‑67 (MQ=255)
                                                                                                              cATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTgg                                                                                                                   <  3:162941/67‑1 (MQ=255)
                                                                                                              cATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgcgg                                                                                                          >  4:1365546/1‑76 (MQ=255)
                                                                                                              cATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgcgg                                                                                                          <  3:1365546/76‑1 (MQ=255)
                                                                                                                ttttGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCt                                          >  2:622608/1‑138 (MQ=255)
                                                                                                                ttttGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCt                                          <  1:622608/138‑1 (MQ=255)
                                                                                                                     tttAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAgcg                                                               >  1:1198474/1‑112 (MQ=255)
                                                                                                                     tttAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAgcg                                                               <  2:1198474/112‑1 (MQ=255)
                                                                                                                     tttAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGcc                                                   <  3:1371348/124‑1 (MQ=255)
                                                                                                                     tttAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGcc                                                   >  4:1371348/1‑124 (MQ=255)
                                                                                                                       tAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGc                        >  1:7911/1‑149 (MQ=255)
                                                                                                                       tAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGc                        <  2:63891/149‑1 (MQ=255)
                                                                                                                         aCGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCcgc                                  <  2:947990/137‑1 (MQ=255)
                                                                                                                         aCGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCCCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGACACAATCGCTTAATCcgc                                  >  1:947990/1‑137 (MQ=255)
                                                                                                                          cGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTaa                     <  1:794811/149‑1 (MQ=255)
                                                                                                                           gggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAAt                                              >  4:470026/1‑123 (MQ=255)
                                                                                                                           gggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAAt                                              <  3:470026/123‑1 (MQ=255)
                                                                                                                           gggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAg                    >  2:695933/1‑149 (MQ=255)
                                                                                                                            ggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAgc                                                                      >  2:611073/1‑98 (MQ=255)
                                                                                                                            ggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAgc                                                                      <  1:611073/98‑1 (MQ=255)
                                                                                                                              tGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGc                                                                                           >  3:469504/1‑75 (MQ=255)
                                                                                                                              tGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGc                                                                                           <  4:469504/75‑1 (MQ=255)
                                                                                                                               gATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCa                >  3:942307/1‑149 (MQ=255)
                                                                                                                                aTCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCcgc                                  <  3:1104302/130‑1 (MQ=255)
                                                                                                                                aTCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCcgc                                  >  4:1104302/1‑130 (MQ=255)
                                                                                                                                aTCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCAc               <  1:337636/149‑1 (MQ=255)
                                                                                                                                aTCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCAc               <  1:481022/149‑1 (MQ=255)
                                                                                                                                   cacaACGTGGGCCAGGCGTCTGGCCCCTGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACAGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAg            >  3:412165/1‑149 (MQ=255)
                                                                                                                                   cacaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTc                          >  3:1183239/1‑135 (MQ=255)
                                                                                                                                   cacaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTc                          <  4:1183239/135‑1 (MQ=255)
                                                                                                                                    acaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGc                                                                                           <  1:369690/69‑1 (MQ=255)
                                                                                                                                    acaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGc                                                                                           >  2:369690/1‑69 (MQ=255)
                                                                                                                                     caACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGtt          <  1:480815/149‑1 (MQ=255)
                                                                                                                                      aaCGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCcgc                                  >  3:558806/1‑124 (MQ=255)
                                                                                                                                      aaCGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCcgc                                  <  4:558806/124‑1 (MQ=255)
                                                                                                                                         atGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAAt                                                      >  4:1181326/2‑101 (MQ=255)
                                                                                                                                         atGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAAt                                                      >  2:907021/2‑101 (MQ=255)
                                                                                                                                         atGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAAt                                                      <  1:907021/100‑1 (MQ=255)
                                                                                                                                         atGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAAt                                                      <  3:1181326/100‑1 (MQ=255)
                                                                                                                                         atGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCAcg      >  1:203066/2‑149 (MQ=255)
                                                                                                                                            ggCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGa   <  1:1213222/149‑1 (MQ=255)
                                                                                                                                             gCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGAt  >  2:319228/1‑149 (MQ=255)
                                                                                                                                              ccAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAgc                                                                <  3:560846/86‑1 (MQ=255)
                                                                                                                                              ccAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAgc                                                                >  4:560846/1‑86 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGAT  >  NC_000913/2581616‑2581905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑