| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,620,220 | A→G | 28.6% | intergenic (‑60/+26) | uraA ← / ← upp | uracil permease/uracil phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,620,220 | 0 | A | G | 28.6% | 17.1 / 6.6 | 14 | intergenic (‑60/+26) | uraA/upp | uracil permease/uracil phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (6/4); new base G (2/2); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01 | |||||||||||
ACATGGCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAA‑AGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTC > NC_000913/2620076‑2620365 | aCATGGCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTTTAAG‑AGTc > 2:916073/1‑149 (MQ=255) tGTAAAGGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCTTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGAATTTAAG‑AGTCGGCt < 2:73318/115‑1 (MQ=255) tGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTTTAAG‑AGTCGGCt > 1:73318/1‑115 (MQ=255) tttCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAA‑AGTCGGCTTTAATTAttt < 3:1142210/109‑1 (MQ=255) tttCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAA‑AGTCGGCTTTAATTAttt > 4:1142210/1‑109 (MQ=255) tcacCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTTTAAG‑AGTCGGCttt < 1:739763/95‑1 (MQ=255) tcacCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACT‑TTAAGAGTCGGCttt > 2:739763/1‑95 (MQ=39) ttATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAA‑AGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAttt > 3:144334/1‑82 (MQ=255) ttATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAA‑AGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAttt < 4:144334/82‑1 (MQ=255) tctTAA‑AGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAAt > 3:1253614/1‑112 (MQ=255) tctTAA‑AGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAAt < 4:1253614/112‑1 (MQ=255) ttAA‑AGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTcc < 1:532241/101‑1 (MQ=255) ttAA‑AGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTcc > 2:532241/1‑101 (MQ=255) ttAA‑AGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTc > 3:346159/1‑149 (MQ=255) | ACATGGCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAA‑AGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTC > NC_000913/2620076‑2620365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |