Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,777,573 A→G 6.0% T41A (ACG→GCG) ‡ ypjF → CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,777,5730AG 6.0% 679.7 / 18.3 197T41A (ACG→GCG) ‡ypjFCP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (88/97);  new base G (6/6);  total (94/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

AACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTC  >  NC_000913/2777425‑2777721
                                                                                                                                                    |                                                                                                                                                    
aaCGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGaca                                                                                                                                                      >  2:480796/1‑149 (MQ=255)
    ctaactaaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGcc                                                                                                                                                  >  4:174762/1‑149 (MQ=255)
      aactaaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGt                                                                                                                                                >  4:1179315/1‑149 (MQ=255)
        ctaaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTc                                                                                                                                              >  1:565262/1‑149 (MQ=255)
         taaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGGCGTGTCGTTCa                                                                                                                                             <  2:1103369/149‑1 (MQ=255)
         taaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCa                                                                                                                                             <  2:1235180/149‑1 (MQ=255)
         taaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCa                                                                                                                                             <  1:480796/149‑1 (MQ=255)
            tttCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGaca                                                                                                                                                      <  2:795818/137‑1 (MQ=255)
            tttCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGtg                                                                                                                                          >  1:1351971/1‑149 (MQ=255)
              tCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGTGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGtgat                                                                                                                                        <  3:1210765/149‑1 (MQ=255)
                aCGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGtgatga                                                                                                                                      >  2:999928/1‑149 (MQ=255)
                 cGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGaa                                                                                                                                     <  3:250418/149‑1 (MQ=255)
                  gagaGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGaaa                                                                                                                                    <  4:406114/149‑1 (MQ=255)
                  gagaGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGaaa                                                                                                                                    <  1:999928/149‑1 (MQ=255)
                  gagaGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGaaa                                                                                                                                    >  4:767560/1‑149 (MQ=255)
                    gagCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACt                                                                                                                                  <  2:565262/149‑1 (MQ=255)
                      gcaagcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGt                                                                                                                                <  4:29436/149‑1 (MQ=255)
                        aagcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGtta                                                                                                                              <  1:1132788/149‑1 (MQ=255)
                          gcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTc                                                                                                                                              <  1:399259/131‑1 (MQ=255)
                          gcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTc                                                                                                                                              >  2:399259/1‑131 (MQ=255)
                               aaCACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGacac                                                                                                                                                     >  2:1338819/1‑119 (MQ=255)
                                acacTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGaa                                                                                                                      >  4:545982/1‑149 (MQ=255)
                                   ctctACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGtta                                                                                                                              >  4:739608/1‑138 (MQ=255)
                                    tctACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTg                                                                                                                                 <  3:759355/134‑1 (MQ=255)
                                    tctACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACata                                                                                                                  >  1:144169/1‑149 (MQ=255)
                                    tctACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACata                                                                                                                  >  4:1102990/1‑149 (MQ=255)
                                          tGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTc                                                                                                                                              >  2:774500/1‑115 (MQ=255)
                                          tGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTc                                                                                                                                              <  1:774500/115‑1 (MQ=255)
                                          tGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGtgatg                                                                                                                                       >  1:260372/1‑122 (MQ=255)
                                             tACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGt                                                                                                                                                >  3:816985/1‑110 (MQ=255)
                                             tACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTg                                                                                                         <  4:678915/149‑1 (MQ=255)
                                              aCAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTgg                                                                                                        >  1:595398/1‑149 (MQ=255)
                                                aaTTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTg                                                                                                         >  3:1376067/1‑146 (MQ=255)
                                                aaTTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTg                                                                                                         >  1:145958/1‑146 (MQ=255)
                                                aaTTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTg                                                                                                         <  4:1376067/146‑1 (MQ=255)
                                                   ttCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCa                                                                                                   <  3:545982/149‑1 (MQ=255)
                                                        aGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGt                                                                                                                                <  4:1203966/115‑1 (MQ=255)
                                                             gcgAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGtta                                                                                                                              >  3:1247066/1‑112 (MQ=255)
                                                                  gCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAg                                                                                    >  4:192571/1‑149 (MQ=255)
                                                                     ctgcctgTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGtt                                                                                                                                               <  3:912442/87‑1 (MQ=255)
                                                                     ctgcctgTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGtt                                                                                                                               >  1:1059591/1‑103 (MQ=255)
                                                                       gcctgTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAAt                                                                               <  2:107058/149‑1 (MQ=255)
                                                                         ctgTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAAc                                                                                                                                   <  1:1089580/95‑1 (MQ=255)
                                                                          tgTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAAtttt                                                                            >  2:49928/1‑149 (MQ=255)
                                                                          tgTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAAtttt                                                                            <  4:317815/149‑1 (MQ=255)
                                                                           gTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTAt                                                                                                     <  4:1228576/123‑1 (MQ=255)
                                                                           gTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTAt                                                                                                     >  3:1228576/1‑123 (MQ=255)
                                                                               ccAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCtggt                                                                       >  2:1654/1‑149 (MQ=255)
                                                                                cAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACata                                                                                                                  >  3:775048/1‑105 (MQ=255)
                                                                                     gCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGaaaa                                                                 >  3:317145/1‑149 (MQ=255)
                                                                                         tCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACatat                                                                                                                 >  3:58886/1‑97 (MQ=255)
                                                                                         tCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACatat                                                                                                                 <  4:58886/97‑1 (MQ=255)
                                                                                           tGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGa                                                           >  3:699009/1‑149 (MQ=255)
                                                                                           tGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGa                                                           >  4:709522/1‑149 (MQ=255)
                                                                                               aaaTGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTg                                                       >  1:611727/1‑149 (MQ=255)
                                                                                                  tGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTa                                                    <  2:611727/149‑1 (MQ=255)
                                                                                                     tACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATg                                                                                                            <  4:277377/90‑1 (MQ=255)
                                                                                                     tACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTa                                                    <  3:216889/146‑1 (MQ=255)
                                                                                                       cTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTAt                                               <  3:190716/149‑1 (MQ=255)
                                                                                                       cTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTAt                                               >  4:834584/1‑149 (MQ=255)
                                                                                                       cTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTAt                                               >  2:925120/1‑149 (MQ=255)
                                                                                                        tGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGt                                                                                                                                           <  1:1404866/56‑1 (MQ=255)
                                                                                                        tGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTAc                                                             >  3:322723/1‑134 (MQ=255)
                                                                                                          acacGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTgg                                                      <  2:1031795/139‑1 (MQ=255)
                                                                                                          acacGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTAc                                                   <  1:124855/142‑1 (MQ=255)
                                                                                                            acGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGATCATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGaaaa                                                                 >  4:80418/1‑126 (MQ=255)
                                                                                                                      gAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCtg                                                                         <  2:274924/108‑1 (MQ=255)
                                                                                                                        acaacaCTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCtg                                                                         >  3:241999/1‑106 (MQ=255)
                                                                                                                         caacaCTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTc                             >  1:1013905/1‑149 (MQ=255)
                                                                                                                          aacaCTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCg                            >  4:1082751/1‑149 (MQ=255)
                                                                                                                           acacTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGt                           <  1:765889/149‑1 (MQ=255)
                                                                                                                           acacTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGt                           >  3:425583/1‑149 (MQ=255)
                                                                                                                            cacTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTg                          <  1:49928/149‑1 (MQ=255)
                                                                                                                             acTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTa                                                    >  3:278722/1‑122 (MQ=255)
                                                                                                                             acTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCg                                             <  2:978243/129‑1 (MQ=255)
                                                                                                                             acTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCg                                             >  1:978243/1‑129 (MQ=255)
                                                                                                                              cTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATGTCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTCGc                        >  4:903547/1‑149 (MQ=255)
                                                                                                                               tATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGaaaa                                                                 >  2:1261613/1‑107 (MQ=255)
                                                                                                                               tATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGaaaa                                                                 <  1:1261613/107‑1 (MQ=255)
                                                                                                                               tATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGca                       >  3:1118017/1‑149 (MQ=255)
                                                                                                                                  ggCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGcaa                      >  2:721638/1‑147 (MQ=255)
                                                                                                                                  ggCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGcaa                      <  1:686330/147‑1 (MQ=255)
                                                                                                                                    ccTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCaacaac                  <  1:925120/149‑1 (MQ=255)
                                                                                                                                     cTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAacaaca                 <  2:13604/149‑1 (MQ=255)
                                                                                                                                      tGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCt                                                                          >  1:1042841/1‑91 (MQ=255)
                                                                                                                                        tcacTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGt                           <  1:818280/135‑1 (MQ=255)
                                                                                                                                        acacTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTgg                                                      <  2:1453343/109‑1 (MQ=255)
                                                                                                                                          acTGAACGGCGTGGCGTTCAGTGATGAAACTTTTATTAAGGAACATATTGATGCTGGTATCACTCTGGCCGAAGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGtctc            >  2:572941/1‑149 (MQ=255)
                                                                                                                                          acTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTAt                                               <  3:874000/114‑1 (MQ=255)
                                                                                                                                          acTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTAt                                               >  4:874000/1‑114 (MQ=255)
                                                                                                                                          acTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGa                                            <  4:243377/117‑1 (MQ=255)
                                                                                                                                          acTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGtctc            >  3:1400834/1‑149 (MQ=255)
                                                                                                                                           cTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGcaa                      <  4:1294396/138‑1 (MQ=255)
                                                                                                                                           cTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGAATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGcaacaa                   >  2:104729/1‑141 (MQ=255)
                                                                                                                                           cTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTcc           <  3:1203080/149‑1 (MQ=255)
                                                                                                                                           cTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTATGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTcccc           <  4:221318/149‑1 (MQ=255)
                                                                                                                                            tGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTgg                                                      <  2:340017/105‑1 (MQ=255)
                                                                                                                                            tGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTgg                                                      >  1:340017/1‑105 (MQ=255)
                                                                                                                                            tGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCa          <  3:713342/149‑1 (MQ=255)
                                                                                                                                                    acGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATc                                              >  4:1385885/1‑105 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTC  >  NC_000913/2777425‑2777721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: