Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,823,839 T→G 6.8% intergenic (‑70/+10) recA ← / ← pncC DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor/nicotinamide‑nucleotide amidohydrolase; NMN amidohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,823,8390TG 6.8% 688.7 / 35.8 191intergenic (‑70/+10)recA/pncCDNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor/nicotinamide‑nucleotide amidohydrolase; NMN amidohydrolase
Reads supporting (aligned to +/- strand):  ref base T (88/90);  new base G (6/7);  total (94/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

Only 100 of 195 total aligned reads displayed.

CCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAG  >  NC_000913/2823696‑2823987
                                                                                                                                               |                                                                                                                                                    
ccTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATAc                                                                                                                                                 <  2:1385556/149‑1 (MQ=255)
ccTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATAc                                                                                                                                                 >  2:346228/1‑149 (MQ=255)
 cTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACa                                                                                                                                                >  2:51012/1‑149 (MQ=255)
  tttACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAg                                                                                                                                               <  1:346228/149‑1 (MQ=255)
    tACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAGTa                                                                                                                                             <  3:938783/149‑1 (MQ=255)
         aaTTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAg                                                                                                                                        >  4:561465/1‑149 (MQ=255)
           ttGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAgtg                                                                                                                                      >  1:955526/1‑149 (MQ=255)
               ttCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGtttt                                                                                                                                  <  3:160189/149‑1 (MQ=255)
               ttCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGtttt                                                                                                                                  <  3:724015/149‑1 (MQ=255)
                tctcAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTg                                                                                                                                 >  1:817626/1‑149 (MQ=255)
                  tcAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTa                                                                                                                               >  1:1033989/1‑149 (MQ=255)
                  tcAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTa                                                                                                                               <  2:374474/149‑1 (MQ=255)
                  tcAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAg                                                                                                                                               >  3:1329728/1‑133 (MQ=255)
                  tcAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAg                                                                                                                                               <  4:1329728/133‑1 (MQ=255)
                     aTCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCaa                                                                                                                                         >  3:1043699/1‑136 (MQ=255)
                     aTCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCaa                                                                                                                                         <  4:1043699/136‑1 (MQ=255)
                               gTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCca                                                                                                                  >  3:31606/1‑149 (MQ=255)
                                  ctgcCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgtt                                                                                                                      >  1:1078459/1‑142 (MQ=255)
                                  ctgcCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgtt                                                                                                                      <  2:1078459/142‑1 (MQ=255)
                                       gccAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACGGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCt                                                                                                          <  2:1249809/149‑1 (MQ=255)
                                           aCGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAg                                                                                                              >  1:1180456/1‑141 (MQ=255)
                                           aCGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAg                                                                                                              <  2:1180456/141‑1 (MQ=255)
                                               tttCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGtt                                                                                                                                    <  4:355371/115‑1 (MQ=255)
                                               tttCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAGTa                                                                                                                                             <  1:265560/106‑1 (MQ=255)
                                                     tttgtttTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCa                                                                                            >  3:1088393/1‑149 (MQ=255)
                                                      ttgtttTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATc                                                                                                                                           >  2:1289109/1‑101 (MQ=255)
                                                      ttgtttTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAg                                                                                           <  3:561465/149‑1 (MQ=255)
                                                                 gATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacga                                                                                <  1:51012/149‑1 (MQ=255)
                                                                  aTAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacgac                                                                               <  2:955526/149‑1 (MQ=255)
                                                                      ccATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATa                                                                                                                                                  <  2:335219/78‑1 (MQ=255)
                                                                      ccATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAg                                                                                                                                               >  4:1376773/1‑81 (MQ=255)
                                                                      ccATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAg                                                                                                                                               <  3:1376773/81‑1 (MQ=255)
                                                                      ccATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGc                                                                                                                    <  1:393257/108‑1 (MQ=255)
                                                                        aTTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGtttt                                                                                                                                  <  2:426037/92‑1 (MQ=255)
                                                                        aTTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGtttt                                                                                                                                  >  1:426037/1‑92 (MQ=255)
                                                                        aTTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAGTAt                                                                                                                                            <  1:126583/82‑1 (MQ=255)
                                                                              aCTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTCTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCAc                                                                   >  3:682086/1‑149 (MQ=255)
                                                                               cTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATa                                                                                                                                                  >  3:359651/1‑69 (MQ=255)
                                                                               cTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACg                                                                  <  2:817626/149‑1 (MQ=255)
                                                                               cTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACg                                                                  >  4:25431/1‑149 (MQ=255)
                                                                                tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgttg                                                                                                                     >  2:1317391/1‑97 (MQ=255)
                                                                                tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACgg                                                                 >  3:460035/1‑149 (MQ=255)
                                                                                tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACgg                                                                 >  4:831988/1‑149 (MQ=255)
                                                                                 ccTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGt                                                                <  2:1033989/149‑1 (MQ=255)
                                                                                  cTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTc                                                               <  1:394715/149‑1 (MQ=255)
                                                                                   tGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAAt                                                                                                     >  4:7913/1‑110 (MQ=255)
                                                                                   tGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAAt                                                                                                     <  3:7913/110‑1 (MQ=255)
                                                                                   tGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTcg                                                              >  1:985503/1‑149 (MQ=255)
                                                                                           cGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGaa                                                      <  4:682086/149‑1 (MQ=255)
                                                                                            gggTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGc                                                                                       >  2:413758/1‑115 (MQ=255)
                                                                                            gggTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGc                                                                                       <  1:413758/115‑1 (MQ=255)
                                                                                              gTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATAc                                                                                               >  2:582670/1‑105 (MQ=255)
                                                                                              gTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATAc                                                                                               <  1:582670/105‑1 (MQ=255)
                                                                                                aaTACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCAtt                                                 <  3:831988/149‑1 (MQ=255)
                                                                                                    ccGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCccg                                             >  4:14270/1‑149 (MQ=255)
                                                                                                     cGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAg                                                                                                                              >  2:618421/1‑67 (MQ=255)
                                                                                                     cGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAg                                                                                                                              <  1:618421/67‑1 (MQ=255)
                                                                                                      ggATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGaa                                                      <  4:625772/138‑1 (MQ=255)
                                                                                                      ggATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGaa                                                      >  3:625772/1‑138 (MQ=255)
                                                                                                      ggATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTccc                                              <  3:1281460/146‑1 (MQ=255)
                                                                                                      ggATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTccc                                              >  4:1281460/1‑146 (MQ=255)
                                                                                                       gATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCt                                                                                                          >  3:1070089/1‑85 (MQ=255)
                                                                                                       gATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCt                                                                                                          <  4:1070089/85‑1 (MQ=255)
                                                                                                        aTAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCgg                                         <  2:985503/149‑1 (MQ=255)
                                                                                                            tCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTaa                                     >  2:401638/1‑149 (MQ=255)
                                                                                                             cAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAAt                                    <  1:222401/149‑1 (MQ=255)
                                                                                                             cAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAAt                                    <  1:1363282/149‑1 (MQ=255)
                                                                                                             cAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAAt                                    <  3:14270/149‑1 (MQ=255)
                                                                                                             cAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAAt                                    <  4:1102037/149‑1 (MQ=255)
                                                                                                                tatGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGcc                                 <  1:1297194/149‑1 (MQ=255)
                                                                                                                tatGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGcc                                 <  4:1088393/149‑1 (MQ=255)
                                                                                                                tatGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGcc                                 <  4:460035/149‑1 (MQ=255)
                                                                                                                    ttCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGaa                                                      >  1:310354/1‑124 (MQ=255)
                                                                                                                    ttCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTc                                                <  1:673310/130‑1 (MQ=255)
                                                                                                                    ttCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTc                                                >  2:673310/1‑130 (MQ=255)
                                                                                                                    ttCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCgg                                         <  2:39396/137‑1 (MQ=255)
                                                                                                                    ttCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCa                             <  1:1391106/149‑1 (MQ=255)
                                                                                                                    ttCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCa                             >  2:11164/1‑149 (MQ=255)
                                                                                                                    ttCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCa                             >  2:1370029/1‑149 (MQ=255)
                                                                                                                      cTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGc                                                                                       <  1:664224/89‑1 (MQ=255)
                                                                                                                      cTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGc                                                                                       >  2:664224/1‑89 (MQ=255)
                                                                                                                            aaGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGcc                                 >  4:1058497/1‑137 (MQ=255)
                                                                                                                            aaGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGc                     >  4:1168314/1‑149 (MQ=255)
                                                                                                                              gCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTc                                                >  2:309723/1‑120 (MQ=255)
                                                                                                                              gCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAg                   >  2:223329/1‑149 (MQ=255)
                                                                                                                                aaTTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTg                 >  3:395536/1‑149 (MQ=255)
                                                                                                                                aaTTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTg                 <  3:1168314/149‑1 (MQ=255)
                                                                                                                                aaTTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTg                 <  3:1120846/149‑1 (MQ=255)
                                                                                                                                 ttgataCTGTATGAGCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCATCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTgg                >  1:747027/4‑149 (MQ=255)
                                                                                                                                 aTTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTgg                >  3:62214/1‑149 (MQ=255)
                                                                                                                                     taCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCggg                      >  1:1016334/1‑139 (MQ=255)
                                                                                                                                     taCTGTATGAGCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCAc                                                                           <  2:949558/86‑1 (MQ=255)
                                                                                                                                     taCTGTATGAGCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCAc                                                                           >  1:949558/1‑86 (MQ=255)
                                                                                                                                      aCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaaaa           <  4:62214/149‑1 (MQ=255)
                                                                                                                                      aCTGTATGAGCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCATCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaaaa           <  2:747027/149‑1 (MQ=255)
                                                                                                                                       cTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGc                                                                                                   <  2:153258/60‑1 (MQ=255)
                                                                                                                                       cTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAg          <  4:395536/149‑1 (MQ=255)
                                                                                                                                        tGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGc         >  3:1099905/1‑149 (MQ=255)
                                                                                                                                          tATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCgg                       <  1:677474/133‑1 (MQ=255)
                                                                                                                                               tCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAg  >  4:71582/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAG  >  NC_000913/2823696‑2823987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: