| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,827,198 | G→C | 5.5% | A269A (GCG→GCC) | srlE → | glucitol/sorbitol‑specific enzyme IIB component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,827,198 | 0 | G | C | 5.5% | 514.0 / 15.0 | 144 | A269A (GCG→GCC) | srlE | glucitol/sorbitol‑specific enzyme IIB component of PTS |
| Reads supporting (aligned to +/- strand): ref base G (67/69); new base C (4/4); total (71/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
Only 100 of 149 total aligned reads displayed.
TCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTAT > NC_000913/2827050‑2827345 | tCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAggcg > 2:870006/1‑149 (MQ=255) ttccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCt < 3:913526/149‑1 (MQ=255) ttccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCt > 2:1005016/1‑149 (MQ=255) ccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGc < 1:1005016/149‑1 (MQ=255) cACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTc > 3:1154604/1‑149 (MQ=255) cACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTc > 3:339192/1‑149 (MQ=255) aCTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCa > 1:748733/1‑149 (MQ=255) cTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTc > 4:664165/1‑144 (MQ=255) cTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTc < 3:664165/144‑1 (MQ=255) tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGAc < 4:900206/140‑1 (MQ=255) tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACtt > 3:898425/1‑142 (MQ=255) tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATc > 3:46718/1‑146 (MQ=255) tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATc < 4:46718/146‑1 (MQ=255) cACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCcggtc < 1:945842/149‑1 (MQ=255) aCCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGAATTGATTGGCGTGAAGATTGGTCTCGGCAATATTCCGCCGCAGCTGGCTTTACCGGCACTGTTTGCCATCAGCGCGCAGGCGGCCTGCGACTTCACCCcggtcg > 3:912574/1‑149 (MQ=255) cTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCcggtcggt > 3:249062/1‑149 (MQ=255) cTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCcggtcggt < 4:1154604/149‑1 (MQ=255) tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGtt > 3:194496/1‑149 (MQ=255) tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGtt > 3:1190090/1‑149 (MQ=255) tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGtt < 4:249062/149‑1 (MQ=255) cTCGGCCCAGGCGCAGTTATCGCAGAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTc < 2:954456/149‑1 (MQ=255) tCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCg > 2:1411946/1‑149 (MQ=255) cGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGc < 4:194496/149‑1 (MQ=255) cccATGCGCAGTTATCGCACAGGTTATCGGCGTATTGATAGGCGTGCCGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGTACTGTTTTCCATCAACGCGCAGGCGGCCTGCGACTTGATCCCGGTCGGTTTGTCGCTgg < 4:912574/149‑1 (MQ=255) ccAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGc > 3:240032/1‑149 (MQ=255) aGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGttt > 4:325095/1‑138 (MQ=255) gcgcAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCcggtcggt > 3:315504/1‑134 (MQ=255) gcgcAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCcggtcggt < 4:315504/134‑1 (MQ=255) aGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGccc < 4:677260/149‑1 (MQ=255) gTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCg > 3:167295/1‑149 (MQ=255) ttATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGt > 3:1221133/1‑149 (MQ=255) aTCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCa < 2:547579/149‑1 (MQ=255) tCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAg < 2:883516/149‑1 (MQ=255) cacaGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACCTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGac > 3:230686/1‑149 (MQ=255) caGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGacac < 2:299019/149‑1 (MQ=255) ggTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTc > 4:1352824/1‑106 (MQ=255) ggTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACCTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACgg < 4:230686/149‑1 (MQ=255) gTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGt > 2:563580/1‑149 (MQ=255) gTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGt < 2:311949/149‑1 (MQ=255) tATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTc > 1:633262/1‑149 (MQ=255) aTCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCCGCCTGCgcgt < 1:953282/100‑4 (MQ=255) aTCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCCGCCTGCgcgt > 2:953282/1‑97 (MQ=255) tCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTcgc < 2:633262/149‑1 (MQ=255) gCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGt < 3:644728/131‑1 (MQ=255) gCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGt > 4:644728/1‑131 (MQ=255) gCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTc < 3:1206223/149‑1 (MQ=255) tATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCg < 3:229049/147‑1 (MQ=255) ttgattgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGgtgt > 1:760551/1‑149 (MQ=255) gattgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTcc < 4:167295/149‑1 (MQ=255) ttgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGaa > 2:213068/1‑116 (MQ=255) ttgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGaa < 1:213068/116‑1 (MQ=255) ttgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGccc > 2:1219129/1‑120 (MQ=255) ttgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTcc > 3:571277/1‑147 (MQ=255) ttgGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTcc < 4:571277/147‑1 (MQ=255) gCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGccc < 4:1353687/117‑1 (MQ=255) gCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGccc > 3:1353687/1‑117 (MQ=255) tGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTa < 3:269527/149‑1 (MQ=255) aGATTGGTCACGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTg > 1:1255198/1‑149 (MQ=255) gATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATcc > 2:182918/1‑83 (MQ=255) aTTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCcggtcggt < 3:1452785/90‑1 (MQ=255) aTTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCcggtcggt > 4:1452785/1‑90 (MQ=255) aTTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCcggtcg > 3:453456/1‑88 (MQ=255) aTTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCcggtcg < 4:453456/88‑1 (MQ=255) aTTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGt > 4:168986/1‑149 (MQ=255) aTTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGt < 1:563580/149‑1 (MQ=255) aTTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGt < 2:760551/149‑1 (MQ=255) tGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGa < 1:1411946/149‑1 (MQ=255) tctcGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGa > 1:1030271/1‑146 (MQ=255) tctcGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGa < 2:1030271/146‑1 (MQ=255) tctcGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGcc > 4:792504/1‑149 (MQ=255) gCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCttt > 3:1036418/1‑149 (MQ=255) tCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCg < 4:353227/149‑1 (MQ=255) gccgcATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCt > 4:1190863/1‑121 (MQ=255) ccgcATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGgcgc < 4:1400421/149‑1 (MQ=255) tCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGa < 3:168986/149‑1 (MQ=255) ggCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTg > 4:1195109/1‑149 (MQ=255) tttACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGttt > 4:1064869/1‑60 (MQ=255) tttACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGttt < 3:1064869/60‑1 (MQ=255) tttACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAc > 4:223326/1‑149 (MQ=255) cGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATc < 2:748733/149‑1 (MQ=255) ggCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCg < 3:1195109/149‑1 (MQ=255) gCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGc > 1:46749/1‑149 (MQ=255) cACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGcc > 2:1324157/1‑149 (MQ=255) cACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGcc < 3:792504/149‑1 (MQ=255) tGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCtttt > 1:1301493/1‑117 (MQ=255) tGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCtttt < 2:1301493/117‑1 (MQ=255) tttGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTAc > 1:629940/1‑100 (MQ=255) tttGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTAc < 2:629940/100‑1 (MQ=255) ttGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGttt < 4:1036418/149‑1 (MQ=255) cATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCgg < 3:1244434/81‑1 (MQ=255) cATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGt > 1:1113106/1‑99 (MQ=255) aTCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGtctc < 4:120646/149‑1 (MQ=255) tCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTGGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTcc < 1:1324157/149‑1 (MQ=255) aaCGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCg > 4:254759/1‑112 (MQ=255) agtcgcAGGCCGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGc < 3:1357467/95‑1 (MQ=255) gtcgcAGGCCGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGa < 4:1104724/126‑1 (MQ=255) tcgcAGGCCGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGttt > 3:1431012/2‑149 (MQ=255) cgcAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGtttt < 1:258585/149‑1 (MQ=255) gcAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTta < 4:1221133/149‑1 (MQ=255) cggcCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTtatctat < 4:239213/149‑1 (MQ=255) | TCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCCATCAACGCGCAGGCGGCCTGCGACTTCATCCCGGTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTAT > NC_000913/2827050‑2827345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |