Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,873,917 G→C 12.7% H26D (CAT→GAT) ‡ cysC ← adenosine 5'‑phosphosulfate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,873,9170GC12.7% 349.6 / 38.2 110H26D (CAT→GAT) ‡cysCadenosine 5'‑phosphosulfate kinase
Reads supporting (aligned to +/- strand):  ref base G (51/45);  new base C (7/7);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.38e-01

Only 100 of 110 total aligned reads displayed.

TCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCA  >  NC_000913/2873772‑2874062
                                                                                                                                                 |                                                                                                                                                 
    tGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtgg                                                                                                                                            >  4:322996/1‑149 (MQ=255)
     gCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCTGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCgtggt                                                                                                                                           <  1:692462/149‑1 (MQ=255)
        tAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTa                                                                                                                                        <  4:850106/149‑1 (MQ=255)
                 gCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtggtg                                                                                                                                          >  1:1146324/1‑138 (MQ=255)
                 gCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtggtg                                                                                                                                          <  2:1146324/138‑1 (MQ=255)
                    aaCATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGgtgt                                                                                                                                         >  4:1164923/1‑136 (MQ=255)
                    aaCATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGgtgt                                                                                                                                         <  3:309028/136‑1 (MQ=255)
                    aaCATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGgtgt                                                                                                                                         <  3:1164923/136‑1 (MQ=255)
                       aTTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgt                                                                                                                             <  4:1191159/145‑1 (MQ=255)
                                     gcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgt                                                                                                                             >  1:1254134/1‑131 (MQ=255)
                                     gcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgt                                                                                                                             <  2:1254134/131‑1 (MQ=255)
                                             gTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGCTTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCa                                                                                                   >  3:122096/1‑149 (MQ=255)
                                              tACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGAt                                                                                                             <  2:466163/138‑1 (MQ=255)
                                              tACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGAt                                                                                                             >  1:466163/1‑138 (MQ=255)
                                                     gCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGt                                                                                           >  4:1372846/1‑149 (MQ=255)
                                                          gTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCg                                                                                      <  3:1372846/149‑1 (MQ=255)
                                                           tttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                        >  4:117256/1‑98 (MQ=255)
                                                           tttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                        <  3:117256/98‑1 (MQ=255)
                                                           tttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtggt                                                                                                                                           >  1:762943/1‑95 (MQ=255)
                                                             tATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATg                                                                                                      <  3:38084/130‑1 (MQ=255)
                                                             tATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATg                                                                                                      >  4:38084/1‑130 (MQ=255)
                                                             tATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAga                                                                                                 <  3:433646/135‑1 (MQ=255)
                                                             tATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAga                                                                                                 >  4:433646/1‑135 (MQ=255)
                                                              aTGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGt                                                                                                                     <  1:951241/114‑1 (MQ=255)
                                                              aTGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGt                                                                                                                     >  2:951241/1‑114 (MQ=255)
                                                               tGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATg                                                                                 >  2:783321/1‑149 (MQ=255)
                                                                 tAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtg                                                                                                                                             >  3:1300605/1‑87 (MQ=255)
                                                                 tAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtg                                                                                                                                             <  4:1300605/87‑1 (MQ=255)
                                                                 tAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCa                                                                               >  2:1410012/1‑149 (MQ=255)
                                                                   aCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgc                                                                             <  1:1410012/149‑1 (MQ=255)
                                                                     gCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCa                                                                                   <  3:392914/141‑1 (MQ=255)
                                                                     gCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCa                                                                                   >  4:392914/1‑141 (MQ=255)
                                                                           tccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCattat                                                                     >  3:808461/1‑149 (MQ=255)
                                                                             cAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGTCAttattt                                                                   <  4:122096/149‑1 (MQ=255)
                                                                                      ggCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATcc                                                               >  1:119485/1‑144 (MQ=255)
                                                                                      ggCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATcc                                                               <  2:119485/144‑1 (MQ=255)
                                                                                             gTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGa                                                                                                                                                  >  1:965029/1‑54 (MQ=255)
                                                                                             gTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGa                                                                                                                                                  <  2:965029/54‑1 (MQ=255)
                                                                                             gTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCatta                                                                      <  1:347620/130‑1 (MQ=255)
                                                                                             gTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCatta                                                                      >  2:347620/1‑130 (MQ=255)
                                                                                              ttGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGAt                                                                                                             >  1:801710/1‑90 (MQ=255)
                                                                                              ttGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGAt                                                                                                             <  2:801710/90‑1 (MQ=255)
                                                                                              ttGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                       >  1:696506/1‑112 (MQ=255)
                                                                                              ttGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                       <  2:696506/112‑1 (MQ=255)
                                                                                              ttGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcgc                                                  >  4:783688/1‑149 (MQ=255)
                                                                                                 aTTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCAgtattta                                                                  <  1:1314309/130‑1 (MQ=255)
                                                                                                 aTTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcgcgcg                                               >  3:387688/1‑149 (MQ=255)
                                                                                                  tttACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGc                                                                                                                          >  1:659036/1‑73 (MQ=255)
                                                                                                  tttACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGc                                                                                                                          <  2:659036/73‑1 (MQ=255)
                                                                                                  tttACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTa                                                                                                        >  4:1467144/1‑91 (MQ=255)
                                                                                                  tttACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTa                                                                                                        <  3:1467144/91‑1 (MQ=255)
                                                                                                  tttACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcgcgcgc                                              >  1:1055613/1‑149 (MQ=255)
                                                                                                        ggACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttg                                                                                                                              >  3:1003653/1‑63 (MQ=255)
                                                                                                        ggACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttg                                                                                                                              <  4:1003653/63‑1 (MQ=255)
                                                                                                          aCCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGt                                      >  4:1363444/1‑149 (MQ=255)
                                                                                                            ccGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCgtggt                                                                                                                                           >  3:367933/1‑46 (MQ=21)
                                                                                                            ccGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCgtggt                                                                                                                                           <  4:367933/46‑1 (MQ=21)
                                                                                                            ccGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgtt                                                                                                                            <  1:64401/61‑1 (MQ=255)
                                                                                                            ccGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgtt                                                                                                                            >  2:64401/1‑61 (MQ=255)
                                                                                                            ccGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGc                                    <  3:783688/149‑1 (MQ=255)
                                                                                                             cGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGtttt                                                                                        >  2:770261/1‑96 (MQ=255)
                                                                                                                 gagGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGaaa                               <  1:783321/149‑1 (MQ=255)
                                                                                                                  agGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATc                                                                <  2:970140/115‑1 (MQ=255)
                                                                                                                  agGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATc                                                                >  1:970140/1‑115 (MQ=255)
                                                                                                                     ccGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                       <  4:684266/89‑1 (MQ=255)
                                                                                                                     ccGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                       >  3:684266/1‑89 (MQ=255)
                                                                                                                       ggTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcg                         >  2:636683/1‑149 (MQ=255)
                                                                                                                       ggTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcg                         >  1:1061962/1‑149 (MQ=255)
                                                                                                                        gTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgt                                                                                                                             >  4:577209/1‑48 (MQ=255)
                                                                                                                        gTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgt                                                                                                                             <  3:577209/48‑1 (MQ=255)
                                                                                                                        gTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcga                        <  1:636683/149‑1 (MQ=255)
                                                                                                                           aaCCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAg                                                                                                                      <  4:1248909/52‑1 (MQ=255)
                                                                                                                           aaCCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAg                                                                                                                      >  3:1248909/1‑52 (MQ=255)
                                                                                                                            aCCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATcc                                                               <  1:589416/106‑1 (MQ=255)
                                                                                                                            aCCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATcc                                                               >  2:589416/1‑106 (MQ=255)
                                                                                                                            aCCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGaa                    >  3:575793/1‑149 (MQ=255)
                                                                                                                              cacaGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAAcc                  <  4:387688/149‑1 (MQ=255)
                                                                                                                               acaGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGACCCa                 >  2:495684/1‑149 (MQ=255)
                                                                                                                                 aGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCaga               <  1:495684/149‑1 (MQ=255)
                                                                                                                                   cACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGc                          <  3:196862/136‑1 (MQ=255)
                                                                                                                                   cACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGc                          >  4:196862/1‑136 (MQ=255)
                                                                                                                                    aCTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATc                                                    <  2:315943/109‑1 (MQ=255)
                                                                                                                                    aCTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATc                                                    >  1:315943/1‑109 (MQ=255)
                                                                                                                                     cTACACCACGGTCATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAt           >  1:179224/1‑149 (MQ=255)
                                                                                                                                      tACACCACGGTCATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTg                                     <  4:1429175/122‑1 (MQ=255)
                                                                                                                                      tACACCACGGTCATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTg                                     >  3:1429175/1‑122 (MQ=255)
                                                                                                                                      tACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAtt          <  2:1055613/149‑1 (MQ=255)
                                                                                                                                       acacCACGGTCATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTc         >  2:1011752/1‑149 (MQ=255)
                                                                                                                                       acacCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTc         <  3:1363444/149‑1 (MQ=255)
                                                                                                                                         accacGGTCATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgcgc                                                                           <  1:673994/81‑1 (MQ=255)
                                                                                                                                         accacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAt           <  3:913330/145‑1 (MQ=255)
                                                                                                                                         accacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCaa       >  3:15270/1‑149 (MQ=255)
                                                                                                                                          ccacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCa                                                                                                   >  3:1016380/1‑56 (MQ=255)
                                                                                                                                          ccacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCa                                                                                                   <  4:1016380/56‑1 (MQ=255)
                                                                                                                                          ccacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTgg                           >  4:163841/1‑128 (MQ=255)
                                                                                                                                          ccacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTgg                           <  3:163841/128‑1 (MQ=255)
                                                                                                                                          ccacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAAt      >  3:1390980/1‑149 (MQ=255)
                                                                                                                                           cacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATcccc                                                             <  4:188122/93‑1 (MQ=255)
                                                                                                                                            acGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTc    <  4:1390980/149‑1 (MQ=255)
                                                                                                                                            acGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTc    >  4:1310421/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCA  >  NC_000913/2873772‑2874062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: