| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,907,998 | A→T | 17.5% | intergenic (‑57/+31) | eno ← / ← pyrG | enolase/CTP synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,907,998 | 0 | A | T | 17.5% | 145.1 / 26.3 | 57 | intergenic (‑57/+31) | eno/pyrG | enolase/CTP synthetase |
| Reads supporting (aligned to +/- strand): ref base A (25/22); new base T (5/5); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-01 | |||||||||||
ACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCAC > NC_000913/2907852‑2908146 | accaccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAgg < 1:736226/149‑1 (MQ=255) ccaccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAggg > 3:152765/1‑149 (MQ=255) cTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAcgc < 2:1055871/149‑1 (MQ=255) aGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTg < 4:152765/149‑1 (MQ=255) aaCAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGtt < 4:993985/131‑1 (MQ=255) aaCAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAACTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGtt > 3:993985/1‑131 (MQ=255) gTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCGAGGGTACGCGt < 3:837799/126‑1 (MQ=255) gTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGt > 4:837799/1‑126 (MQ=255) gatgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAgg > 3:538891/1‑98 (MQ=255) gatgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAgg < 4:538891/98‑1 (MQ=255) atgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGtt > 1:198240/1‑106 (MQ=255) atgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGtt < 2:198240/106‑1 (MQ=255) atgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAgg < 1:1309268/97‑1 (MQ=255) atgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAgg > 2:1309268/1‑97 (MQ=255) tttttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGtt > 4:138913/1‑87 (MQ=255) tttttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGtt < 3:138913/87‑1 (MQ=255) gATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCTAGACAAACAACGCGTACCCTGGGTACGCGt > 1:947582/1‑79 (MQ=255) gATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGt < 2:947582/79‑1 (MQ=255) ttAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCggg > 2:395537/1‑149 (MQ=255) tttCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCGAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCAgg > 4:981188/1‑104 (MQ=255) tttCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTgg < 3:981188/104‑1 (MQ=255) tCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACaaa > 3:313013/1‑122 (MQ=255) tCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACaaa < 4:313013/122‑1 (MQ=255) tCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAAc < 4:662122/132‑1 (MQ=255) tCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAAc > 3:662122/1‑132 (MQ=255) tCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGt > 2:104541/1‑52 (MQ=255) tCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGt < 1:104541/52‑1 (MQ=255) tCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTg < 4:1322828/93‑1 (MQ=255) tCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTg > 3:1322828/1‑93 (MQ=255) cTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAg > 1:1465516/1‑149 (MQ=255) ttAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACttt < 2:179717/60‑1 (MQ=255) ttAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACttt > 1:179717/1‑60 (MQ=255) ttAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTttacttac < 1:508343/69‑1 (MQ=255) ttAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTttacttac > 2:508343/1‑69 (MQ=255) ttAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGt > 1:139564/1‑149 (MQ=255) aaCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTaaa < 1:395537/149‑1 (MQ=255) aCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCt > 3:1291566/1‑110 (MQ=255) aCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCt < 4:1291566/110‑1 (MQ=255) aCTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGa < 2:139564/149‑1 (MQ=255) aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGGAAACTCCGGATGGaa > 2:424437/1‑149 (MQ=255) aaacaaCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGa < 3:179039/122‑1 (MQ=255) aaacaaCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGa > 4:179039/1‑122 (MQ=255) gcaacGCGTACCCTGGGTACGCGTTGTCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTc > 1:249365/2‑71 (MQ=38) gcaacGCGTACCCTGGGTACGCGTTGTCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTc < 2:249365/70‑1 (MQ=255) caacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACAATCACGTGGAGTAGAAGTAAAGTCCGGATGGAACTGGCaa < 1:424437/149‑1 (MQ=255) aacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAg > 4:229638/1‑149 (MQ=255) gcgTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCt < 1:1228838/76‑1 (MQ=255) gcgTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCt > 2:1228838/1‑76 (MQ=255) gcgTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCggg > 3:1426221/1‑100 (MQ=255) gcgTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCggg < 4:1426221/100‑1 (MQ=255) ccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGa < 3:486388/127‑1 (MQ=255) ccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGa > 4:486388/1‑127 (MQ=255) ccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAAcc > 4:187331/1‑149 (MQ=255) ccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAAcc > 1:556863/1‑149 (MQ=255) ccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAAcc > 1:1247987/1‑149 (MQ=255) cAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGa > 3:918610/1‑108 (MQ=255) cAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGa < 4:918610/108‑1 (MQ=255) aGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGaa < 2:1374864/113‑1 (MQ=255) aGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGaa > 1:1374864/1‑113 (MQ=255) aGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCAc > 2:1073723/1‑149 (MQ=255) aGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCAc > 3:216898/1‑149 (MQ=255) | ACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCAC > NC_000913/2907852‑2908146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |