Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,926,036 A→C 8.1% E230A (GAA→GCA)  queF → 7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,926,0360AC 8.1% 549.7 / 36.8 160E230A (GAA→GCA) queF7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)
Reads supporting (aligned to +/- strand):  ref base A (74/73);  new base C (7/6);  total (81/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-02

Only 100 of 161 total aligned reads displayed.

TTGTCAGCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGA  >  NC_000913/2925889‑2926181
                                                                                                                                                   |                                                                                                                                                 
ttGTCAGCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGaa                                                                                                                                                  <  1:439135/149‑1 (MQ=255)
     aGCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTg                                                                                                                                             >  3:865954/1‑149 (MQ=255)
      gCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGc                                                                                                                                            >  2:863215/1‑149 (MQ=255)
       ccACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCg                                                                                                                                           <  3:407814/149‑1 (MQ=255)
         aCCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                                                                         >  3:789033/1‑149 (MQ=255)
            tgctgAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGaa                                                                                                                                      <  4:1403048/149‑1 (MQ=255)
             gctgAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAAc                                                                                                                                     <  4:938220/149‑1 (MQ=255)
                 aaaTCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCAt                                                                                                                                 <  3:792786/149‑1 (MQ=255)
                    tCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGt                                                                                                                                              <  3:1177475/133‑1 (MQ=255)
                      aaaCTGCCTGATCACCCATCAACCAGCTTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGt                                                                                                                                          >  4:1251562/1‑135 (MQ=255)
                      aaaCTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGaa                                                                                                                                      <  1:177269/139‑1 (MQ=255)
                      aaaCTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGaa                                                                                                                                      >  2:177269/1‑139 (MQ=255)
                      aaaCTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGt                                                                                                                                          <  3:1251562/135‑1 (MQ=255)
                       aaCTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTaa                                                                                                                           >  3:263390/1‑149 (MQ=255)
                       aaCTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTaa                                                                                                                           <  1:123291/149‑1 (MQ=255)
                           gCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGAc                                                                                                                       <  4:865954/149‑1 (MQ=255)
                             cTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCt                                                                                                                     >  4:492514/1‑149 (MQ=255)
                              tGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTaa                                                                                                                           >  2:436828/1‑142 (MQ=255)
                               gATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGt                                                                                                                   >  2:146195/1‑149 (MQ=255)
                                 tCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTa                                                                                                                 <  1:146195/149‑1 (MQ=255)
                                       aTCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTc                                                                                                           <  4:263390/149‑1 (MQ=255)
                                          aaCCAGCTTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGAc                                                                                                                       >  4:1264453/1‑134 (MQ=255)
                                          aaCCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCtt                                                                                                                              >  2:700601/1‑127 (MQ=255)
                                          aaCCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgc                                                                                                        >  3:1315513/1‑149 (MQ=255)
                                           aCCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCa                                                                                                                                  >  3:1265442/1‑122 (MQ=255)
                                               gATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTa                                                                                                                 >  3:122567/1‑135 (MQ=255)
                                                       ttCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTc                                                                                                           <  4:930948/133‑1 (MQ=255)
                                                       ttCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTc                                                                                                           >  3:930948/1‑133 (MQ=255)
                                                       ttCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAAt                                                                                           >  1:758197/1‑149 (MQ=255)
                                                          gCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGa                                                                                        <  2:758197/149‑1 (MQ=255)
                                                          gCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGa                                                                                        <  1:863215/149‑1 (MQ=255)
                                                          gCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGa                                                                                        <  4:789033/149‑1 (MQ=255)
                                                           cTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAAtt                                                                                          >  4:54634/1‑146 (MQ=255)
                                                            tCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGc                                                                                                              >  2:972731/1‑125 (MQ=255)
                                                            tCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGc                                                                                                              <  1:972731/125‑1 (MQ=255)
                                                             ccAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCg                                                                                     >  2:51734/1‑149 (MQ=255)
                                                               aaaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGtt                                                                                   >  3:568186/1‑149 (MQ=255)
                                                                aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGaaaaa                                                                                            <  2:1337714/139‑1 (MQ=255)
                                                                aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGaaaaa                                                                                            >  1:1337714/1‑139 (MQ=255)
                                                                aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTCCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGaaaaa                                                                                            <  4:1284395/139‑1 (MQ=255)
                                                                  ttCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTAc                                                                                <  3:492514/149‑1 (MQ=255)
                                                                     aGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCa                                                                             <  4:206951/149‑1 (MQ=255)
                                                                       tATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                                                                         <  1:973773/87‑1 (MQ=255)
                                                                       tATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                                                                         >  2:973773/1‑87 (MQ=255)
                                                                            tGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAAt                                                                                           <  2:1133478/128‑1 (MQ=255)
                                                                            tGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAAt                                                                                           >  1:1133478/1‑128 (MQ=255)
                                                                                 gCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGa                                                                                        >  3:981184/1‑126 (MQ=255)
                                                                                 gCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgt                                                                 >  4:631566/1‑149 (MQ=255)
                                                                                            agagAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCt                                                      >  4:218323/1‑149 (MQ=255)
                                                                                            agagAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCt                                                      <  1:51734/149‑1 (MQ=255)
                                                                                               gaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGa                                                   >  3:1080234/1‑149 (MQ=255)
                                                                                                     ctgctgCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTaa                                             <  2:870941/149‑1 (MQ=255)
                                                                                                         tgcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATAc                                                                     <  1:616716/121‑1 (MQ=255)
                                                                                                         tgcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTg                                                     <  3:1467989/137‑1 (MQ=255)
                                                                                                            gTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTgg                                      >  4:976180/1‑149 (MQ=255)
                                                                                                                  tGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGt                                >  1:79202/1‑149 (MQ=255)
                                                                                                                     tctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAAt                                                                                                                          >  1:1410767/1‑56 (MQ=255)
                                                                                                                     tctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAAt                                                                                                                          <  2:1410767/56‑1 (MQ=255)
                                                                                                                     tctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGt                                        >  4:722935/1‑138 (MQ=255)
                                                                                                                     tctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAAt                             <  1:448307/149‑1 (MQ=255)
                                                                                                                      ctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATa                            <  3:953467/149‑1 (MQ=255)
                                                                                                                       tcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCt                                                                                                             >  1:552159/1‑67 (MQ=255)
                                                                                                                       tcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCt                                                                                                             <  2:552159/67‑1 (MQ=255)
                                                                                                                          ttCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGa                        >  1:376815/1‑149 (MQ=255)
                                                                                                                              gTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGAtttt                    >  2:494215/1‑149 (MQ=255)
                                                                                                                              gTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGAtttt                    >  4:1452333/1‑149 (MQ=255)
                                                                                                                                 atcaCAACGAGTTCCATGAACAGTGCGTGGAACGCATCTTTAATGAGCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTc                 <  4:1194999/149‑1 (MQ=255)
                                                                                                                                 atcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGa                                                   <  3:140413/115‑1 (MQ=255)
                                                                                                                                 atcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGa                                                   >  4:140413/1‑115 (MQ=255)
                                                                                                                                 atcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTc                 <  3:218323/149‑1 (MQ=255)
                                                                                                                                  tcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGc                                                                                      <  2:922691/79‑1 (MQ=255)
                                                                                                                                  tcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGc                                                                                      >  1:922691/1‑79 (MQ=255)
                                                                                                                                  tcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTcc                >  4:233388/1‑149 (MQ=255)
                                                                                                                                  tcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTcc                >  1:1013162/1‑149 (MQ=255)
                                                                                                                                   cacaACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGa                                                                                        >  4:118067/1‑76 (MQ=255)
                                                                                                                                   cacaACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGa                                                                                        <  3:118067/76‑1 (MQ=255)
                                                                                                                                      aaCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGa                                                   >  4:633654/1‑110 (MQ=255)
                                                                                                                                      aaCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGa                                                   <  3:633654/110‑1 (MQ=255)
                                                                                                                                      aaCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGAttt                     <  3:262367/140‑1 (MQ=255)
                                                                                                                                      aaCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGAttt                     >  4:262367/1‑140 (MQ=255)
                                                                                                                                      aaCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTc                 <  1:864947/144‑1 (MQ=255)
                                                                                                                                       aCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGtt                                                                         >  4:1007836/1‑87 (MQ=255)
                                                                                                                                        cGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACAt                                                >  4:162015/1‑111 (MQ=255)
                                                                                                                                         gAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGAc         <  3:1452333/149‑1 (MQ=255)
                                                                                                                                           gTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAAtt                                                                                          >  3:520733/1‑66 (MQ=37)
                                                                                                                                           gTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGt                                <  1:308833/124‑1 (MQ=255)
                                                                                                                                           gTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGt                                >  2:308833/1‑124 (MQ=255)
                                                                                                                                           gTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACca       >  1:1065474/1‑149 (MQ=255)
                                                                                                                                           gTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTaa                                             >  3:173177/1‑111 (MQ=255)
                                                                                                                                           gTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTaa                                             <  4:173177/111‑1 (MQ=255)
                                                                                                                                            ttCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGa                        >  1:1389781/1‑131 (MQ=255)
                                                                                                                                            ttCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGa                        <  2:1389781/131‑1 (MQ=255)
                                                                                                                                            ttCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACcac      >  2:1108727/1‑149 (MQ=255)
                                                                                                                                            ttCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGa                                                                                        <  1:404379/67‑1 (MQ=255)
                                                                                                                                            ttCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGa                                                                                        >  2:404379/1‑67 (MQ=255)
                                                                                                                                            ttCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATc            >  3:225734/1‑143 (MQ=255)
                                                                                                                                              ccACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGAt                       >  4:1084865/1‑130 (MQ=255)
                                                                                                                                               cACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAAtt                                                                                          >  4:42393/1‑62 (MQ=255)
                                                                                                                                                aCGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGa  >  3:654597/1‑149 (MQ=255)
                                                                                                                                                aCGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCACATCGACCACAAGa  >  1:818707/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTGTCAGCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGA  >  NC_000913/2925889‑2926181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: