| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,020,512 | A→C | 17.8% | intergenic (+23/‑28) | ssnA → / → ygfM | putative chlorohydrolase/aminohydrolase/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,020,512 | 0 | A | C | 17.8% | 120.0 / 23.4 | 53 | intergenic (+23/‑28) | ssnA/ygfM | putative chlorohydrolase/aminohydrolase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (21/20); new base C (4/5); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-01 | |||||||||||
CGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCC > NC_000913/3020366‑3020657 | cGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAgg < 3:216375/149‑1 (MQ=255) gATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGaa > 4:862800/1‑149 (MQ=255) ggTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGC‑GGGAagag < 4:1146704/149‑1 (MQ=255) ggTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑TGCGGGAagag < 2:484610/149‑1 (MQ=255) tCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGa > 3:697865/1‑143 (MQ=255) tCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGa < 4:697865/143‑1 (MQ=255) aaTGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATACCCTCTTCCC‑TGCGGGAAGAgggca < 2:96903/149‑3 (MQ=255) gtgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATaa < 3:862800/149‑1 (MQ=255) aTGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAg > 1:617890/1‑129 (MQ=255) aTGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAg < 2:617890/129‑1 (MQ=255) ggTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAgg > 1:44777/1‑128 (MQ=255) ggTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAgg < 2:44777/128‑1 (MQ=255) tGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAgat > 4:526504/1‑149 (MQ=255) gAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAgatg < 3:526504/149‑1 (MQ=255) aGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAagag < 1:1332716/125‑1 (MQ=255) aGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAagag > 2:1332716/1‑125 (MQ=255) gTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCa < 4:1058961/112‑1 (MQ=255) gTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCa > 3:1058961/1‑112 (MQ=255) gTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAtt > 3:268407/1‑149 (MQ=255) tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAg > 2:793660/1‑108 (MQ=255) tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAg < 1:793660/108‑1 (MQ=255) aCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa < 4:268407/149‑1 (MQ=255) ttCGATTGCGATTCCATTTATGCACCAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑CGCGGGAAGAgg < 4:134609/110‑1 (MQ=255) ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTaaa > 2:962551/1‑116 (MQ=255) ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTaaa < 1:962551/116‑1 (MQ=255) ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑CGCGGGAAGAgg > 3:134609/1‑110 (MQ=255) aTTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAg > 3:495059/1‑116 (MQ=255) aTTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAg < 4:495059/116‑1 (MQ=255) aTTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAg < 3:574078/110‑1 (MQ=255) aTTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAg > 4:574078/1‑110 (MQ=255) aGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGAct < 2:148641/130‑1 (MQ=255) aGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGAct > 1:148641/1‑130 (MQ=255) gAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt > 1:691194/1‑103 (MQ=255) gAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt < 2:691194/103‑1 (MQ=255) gCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATACCCTCTTCCCTGC‑GGGAAGAgggc < 4:672652/76‑2 (MQ=37) gCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATACCCTCTTCCCTGC‑GGGAAGAgggc > 3:672652/1‑75 (MQ=37) ccGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑TGCGGGAaga > 2:675192/1‑71 (MQ=39) ccAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt > 4:994873/1‑91 (MQ=255) ccAGTATGTGGCGGCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt < 3:994873/91‑1 (MQ=255) gatggatgCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATaa > 2:422651/1‑60 (MQ=255) gatggatgCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATaa < 1:422651/60‑1 (MQ=255) gATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAg < 1:1060820/36‑1 (MQ=255) gATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAg > 2:1060820/1‑36 (MQ=255) gATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAg > 4:794826/1‑117 (MQ=255) gATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAg < 3:794826/117‑1 (MQ=255) ccTCTTCCC‑TGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGaa < 2:1279567/92‑1 (MQ=255) ccTCTTCCC‑TGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGaa > 1:1279567/1‑92 (MQ=255) ctctTCCC‑TGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCt < 2:1002189/117‑1 (MQ=255) ctctTCCC‑TGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCt > 1:1002189/1‑117 (MQ=255) tctTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGaa < 1:825126/109‑1 (MQ=255) tctTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGaa > 2:825126/1‑109 (MQ=255) c‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCgg > 1:587631/1‑120 (MQ=255) c‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCgg < 2:587631/120‑1 (MQ=255) c‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAAccc > 4:591117/1‑149 (MQ=255) c‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAATGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAAccc > 4:36973/1‑149 (MQ=255) | CGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCC > NC_000913/3020366‑3020657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |