Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,021,916 T→G 5.8% C201G (TGC→GGC)  xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,021,9160TG 5.8% 369.0 / 12.6 104C201G (TGC→GGC) xdhDputative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
Reads supporting (aligned to +/- strand):  ref base T (50/48);  new base G (3/3);  total (53/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

Only 100 of 108 total aligned reads displayed.

ATAATCCGCAGGCCACCATTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCG  >  NC_000913/3021773‑3022063
                                                                                                                                               |                                                                                                                                                   
ataatCCGCAGGCCACCATTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTc                                                                                                                                                <  1:449636/149‑1 (MQ=255)
    tCCGCAGGCCACCATTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTa                                                                                                                                            >  1:479149/1‑149 (MQ=255)
        cAGGCCACCATTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAAt                                                                                                                                        >  4:1286172/1‑149 (MQ=255)
           gCCACCATTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGtt                                                                                                                                     <  1:858416/149‑1 (MQ=255)
             caccaTTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGc                                                                                                                                                   >  2:1317522/1‑133 (MQ=255)
             caccaTTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGc                                                                                                                                                   <  1:1317522/133‑1 (MQ=255)
                caTTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGc                                                                                                                                                   >  4:616162/1‑130 (MQ=255)
                caTTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGc                                                                                                                                                   <  3:616162/130‑1 (MQ=255)
                  ttGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGc                                                                                                                              >  3:720442/1‑149 (MQ=255)
                                  tttCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCAc                                                                                                              >  1:970253/1‑149 (MQ=255)
                                      cGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGAt                                                                                                          <  2:479149/149‑1 (MQ=255)
                                       gTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTg                                                                                                                                                    <  2:1089134/106‑1 (MQ=255)
                                       gTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTg                                                                                                                                                    >  1:1089134/1‑106 (MQ=255)
                                       gTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTaaaa                                                                                                                                         >  2:850210/1‑117 (MQ=255)
                                       gTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTaaaa                                                                                                                                         <  1:850210/117‑1 (MQ=255)
                                            gagcTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAAACCTGCTATGTTGAAGACCGTGAAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCCAACGCTCACGCACTGATTACTCa                                                                                                    >  4:692985/4‑149 (MQ=255)
                                               cTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCAGGCGTCagcc                                                                                                                                            <  1:9812/106‑4 (MQ=255)
                                               cTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCAGGCGTCagcc                                                                                                                                            <  1:294367/106‑4 (MQ=255)
                                               cTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCAGGCGTCagcc                                                                                                                                            >  2:294367/1‑103 (MQ=255)
                                                 aGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTgg                                                                                               >  1:1390320/1‑149 (MQ=255)
                                                  gAAGTCATTGGTGAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCACGCGAAACTCTGTTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACCGATTACTCATCTGGa                                                                                              <  3:692985/149‑1 (MQ=255)
                                                  gAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGa                                                                                              >  2:1457210/1‑149 (MQ=255)
                                                  gAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGa                                                                                              <  4:720442/149‑1 (MQ=255)
                                                     gTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGt                                                                                           >  2:358004/1‑149 (MQ=255)
                                                           ggTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGAt                                                                                                          <  2:936951/128‑1 (MQ=255)
                                                           ggTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGAt                                                                                                          >  1:936951/1‑128 (MQ=255)
                                                            gTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGc                                                                                                                      <  4:1347658/115‑1 (MQ=255)
                                                            gTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCaaa                                                                                    <  3:1286172/149‑1 (MQ=255)
                                                            gTAAGCATGATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGc                                                                                                                      >  3:1347658/1‑115 (MQ=255)
                                                             tAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGa                                                                                              <  1:1330893/138‑1 (MQ=255)
                                                             tAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGa                                                                                              >  2:1330893/1‑138 (MQ=255)
                                                             tAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCaaa                                                                                    <  4:1024600/148‑1 (MQ=255)
                                                             tAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCaaa                                                                                    >  3:1024600/1‑148 (MQ=255)
                                                              aaGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGc                                                                                  <  1:358004/149‑1 (MQ=255)
                                                                  aTTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGaa                                                                              >  1:753278/1‑149 (MQ=255)
                                                                  aTTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGaa                                                                              <  2:1390320/149‑1 (MQ=255)
                                                                       ccTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGc                                                                                                                              >  2:539763/1‑96 (MQ=255)
                                                                       ccTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGc                                                                                                                              <  1:539763/96‑1 (MQ=255)
                                                                       ccTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCtt                                                                         <  2:970253/149‑1 (MQ=255)
                                                                        cTAAACCTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTAc                                                                                                       <  1:186184/118‑1 (MQ=255)
                                                                        cTAAAACTGATGCCGCGAAAATGTTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTc                                                                                          <  4:1198298/131‑1 (MQ=255)
                                                                        cTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTAc                                                                                                       >  2:186184/1‑118 (MQ=255)
                                                                        cTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCt                                                                                                 <  3:597358/124‑1 (MQ=255)
                                                                        cTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCt                                                                                                 >  4:597358/1‑124 (MQ=255)
                                                                        cTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGt                                                                                           <  1:1089127/130‑1 (MQ=255)
                                                                        cTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGt                                                                                           >  2:1089127/1‑130 (MQ=255)
                                                                        cTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTc                                                                                          >  3:1198298/1‑131 (MQ=255)
                                                                        cTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACAGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTa                                                                        >  3:226073/1‑149 (MQ=255)
                                                                          aaaaCTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGc                                                                                                                                                   >  2:1414150/1‑72 (MQ=255)
                                                                          aaaaCTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGc                                                                                                                                                   <  1:1414150/72‑1 (MQ=255)
                                                                            aaCTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCgg                                                                    >  1:1036849/1‑149 (MQ=255)
                                                                                 aTGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGcgtc                                                               <  2:753278/149‑1 (MQ=255)
                                                                                      gcgAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCAt                                                                                                   <  1:300022/108‑1 (MQ=255)
                                                                                      gcgAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCa                                                                                         <  4:147661/118‑1 (MQ=255)
                                                                                      gcgAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCa                                                                                         >  3:147661/1‑118 (MQ=255)
                                                                                      gcgAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGaa                                                                              <  1:276752/129‑1 (MQ=255)
                                                                                      gcgAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGaa                                                                              >  2:276752/1‑129 (MQ=255)
                                                                                      gcgAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCa                                                          >  1:1278651/1‑149 (MQ=255)
                                                                                      gcgAAAATGGTGCAGGCGAAACCATGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCAt                                                                                                   >  2:300022/1‑108 (MQ=255)
                                                                                                tGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATg                                                                                                                                       >  2:591820/1‑62 (MQ=255)
                                                                                                  cAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCa                                              >  2:226588/1‑149 (MQ=255)
                                                                                                       gAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGa                                         >  2:1181170/1‑149 (MQ=255)
                                                                                                            ccTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCGCTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGcgtcgt                                                             <  1:1404934/124‑1 (MQ=255)
                                                                                                            ccTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGcgtcgt                                                             >  2:1404934/1‑124 (MQ=255)
                                                                                                              tGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGccc                                  >  3:869030/1‑149 (MQ=255)
                                                                                                               gCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCg                                 <  1:226588/149‑1 (MQ=255)
                                                                                                                   tGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGt                                                                                                                                 >  4:33835/1‑49 (MQ=255)
                                                                                                                   tGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGt                                                                                                                                 <  1:647402/49‑1 (MQ=255)
                                                                                                                   tGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGt                                                                                                                                 >  2:647402/1‑49 (MQ=255)
                                                                                                                   tGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGt                                                                                                                                 <  3:33835/49‑1 (MQ=255)
                                                                                                                       gAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATc                                                                                                  >  1:1136552/1‑76 (MQ=255)
                                                                                                                       gAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATc                                                                                                  <  2:1136552/76‑1 (MQ=255)
                                                                                                                        aaGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATctac                        <  1:1181170/149‑1 (MQ=255)
                                                                                                                         aGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTc                                                           <  3:1042527/113‑1 (MQ=255)
                                                                                                                         aGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTc                                                           >  4:1042527/1‑113 (MQ=255)
                                                                                                                         aGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGttatt                                                   >  1:278936/1‑121 (MQ=255)
                                                                                                                          gACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATctacta                      >  4:83086/1‑149 (MQ=255)
                                                                                                                           aCCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGAt                                                                                             >  4:1407219/1‑77 (MQ=255)
                                                                                                                           aCCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGAt                                                                                             <  3:1407219/77‑1 (MQ=255)
                                                                                                                            ccGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATCTACtata                    <  2:1278651/149‑1 (MQ=255)
                                                                                                                             cgcgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCAc                                             <  1:679115/123‑1 (MQ=255)
                                                                                                                             cgcgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCAc                                             >  2:679115/1‑123 (MQ=255)
                                                                                                                             cgcgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATct                          <  4:286457/142‑1 (MQ=255)
                                                                                                                                 tAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGccc                                  <  3:946924/130‑1 (MQ=255)
                                                                                                                                  aaCGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACg                                                        >  2:181374/1‑107 (MQ=255)
                                                                                                                                  aaCGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACg                                                        >  1:240868/1‑107 (MQ=255)
                                                                                                                                  aaCGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACg                                                        <  2:240868/107‑1 (MQ=255)
                                                                                                                                    ttaatGACGCAGGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTc                                                           >  3:1325006/5‑102 (MQ=255)
                                                                                                                                        tGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGa                                                                                              <  4:1054414/63‑1 (MQ=255)
                                                                                                                                        tGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGa                                                                                              >  3:1054414/1‑63 (MQ=255)
                                                                                                                                        tGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATAccc                 >  1:1176800/1‑140 (MQ=255)
                                                                                                                                        tGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATAccc                 <  2:1176800/140‑1 (MQ=255)
                                                                                                                                        tGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTc        <  1:1457210/149‑1 (MQ=255)
                                                                                                                                           cGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCaga     <  4:869030/149‑1 (MQ=255)
                                                                                                                                              cTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACg                                                        >  2:500948/1‑95 (MQ=255)
                                                                                                                                              cTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACg                                                        <  1:500948/95‑1 (MQ=255)
                                                                                                                                               tGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCa                                                          >  3:1251593/1‑92 (MQ=255)
                                                                                                                                               tGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCa                                                          <  4:1251593/92‑1 (MQ=255)
                                                                                                                                               tGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGtta                                                     <  4:1344189/97‑1 (MQ=255)
                                                                                                                                               tGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGtta                                                     >  3:1344189/1‑97 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
ATAATCCGCAGGCCACCATTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCG  >  NC_000913/3021773‑3022063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: