Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,082,709 C→A 6.5% intergenic (+38/+168) loiP → / ← speB Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein/agmatinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,082,7090CA 6.5% 376.3 / 13.7 107intergenic (+38/+168)loiP/speBPhe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein/agmatinase
Reads supporting (aligned to +/- strand):  ref base C (50/50);  new base A (4/3);  total (54/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

Only 100 of 107 total aligned reads displayed.

GGCAAAACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTT  >  NC_000913/3082563‑3082852
                                                                                                                                                  |                                                                                                                                               
 gCAAAACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCAcg                                                                                                                                              >  1:1098459/1‑149 (MQ=255)
  cAAAACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCAcgc                                                                                                                                             <  3:1039967/149‑1 (MQ=255)
  cAAAACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCAcgc                                                                                                                                             <  3:710374/149‑1 (MQ=255)
   aaaaCTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCAcgcc                                                                                                                                            >  1:57775/1‑149 (MQ=255)
      aCTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCa                                                                                                                                         <  4:1287353/149‑1 (MQ=255)
        tGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATc                                                                                                                                       >  2:985785/1‑149 (MQ=255)
          gaagaaGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATGCGGCGTGAAcgcctta                                                                                                                                         >  2:1000069/1‑142 (MQ=255)
           aagaagGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGa                                                                                                                                    >  1:399134/1‑149 (MQ=255)
           aagaagGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGa                                                                                                                                    <  2:57775/149‑1 (MQ=255)
             gaagGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACa                                                                                                                                  >  2:331443/1‑149 (MQ=255)
             gaagGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCAGAACGCGCCCAGCATATTTGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACa                                                                                                                                  >  3:913657/1‑149 (MQ=255)
               agGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATGCGGCGTGAACGCCTTATCTGAcc                                                                                                                                  >  2:687085/1‑146 (MQ=255)
                 gTCGCCAAAGCTCAATGTTTGACGATCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGaa                                                                                                                              <  3:955320/149‑1 (MQ=255)
                   cGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATg                                                                                                                            <  1:926329/149‑1 (MQ=255)
                    gCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATgg                                                                                                                           <  1:985785/149‑1 (MQ=255)
                        aaGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATGCGGCGTGAACGCCTTATCTGa                                                                                                                                    <  1:498825/136‑1 (MQ=255)
                        aaGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATGCGGCGTGAACGCCTTATCTGa                                                                                                                                    >  2:498825/1‑136 (MQ=255)
                           cTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACAttt                                                                                                                    <  1:331443/149‑1 (MQ=255)
                              aaTGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTATGTCAGATGCGGCGTGAACGCCTTATCTGAcc                                                                                                                                  >  2:399189/1‑131 (MQ=38)
                              aaTGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTATGTCAGATGCGGCGTGAACGCCTTATCTGAcc                                                                                                                                  <  1:399189/132‑2 (MQ=38)
                              aaTGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGtt                                                                                                                 >  2:1203607/1‑149 (MQ=255)
                              aaTGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGtt                                                                                                                 <  1:1203607/149‑1 (MQ=255)
                               aTGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCAc                                                                                                                                               <  3:587398/118‑1 (MQ=255)
                               aTGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCAc                                                                                                                                               >  4:587398/1‑118 (MQ=255)
                                tGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCAcgccgc                                                                                                                                          <  1:730677/122‑1 (MQ=255)
                                tGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCAcgccgc                                                                                                                                          >  2:730677/1‑122 (MQ=255)
                                 gTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTAcc                                                                                                              >  2:280135/1‑149 (MQ=255)
                                     gacgacCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTtgt                                                                                                          <  2:399134/149‑1 (MQ=255)
                                       cgacCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTgc                                                                                                        <  1:1372124/149‑1 (MQ=255)
                                        gacCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTgcg                                                                                                       >  4:76409/1‑149 (MQ=255)
                                          ccATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTgc                                                                                                        <  3:1369418/146‑1 (MQ=255)
                                          ccATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTgc                                                                                                        >  4:1369418/1‑146 (MQ=255)
                                           cATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGa                                                                                                                                    <  4:934994/117‑1 (MQ=255)
                                           cATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGa                                                                                                                                    >  3:934994/1‑117 (MQ=255)
                                           cATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCAt                                                                                                    >  4:167727/1‑149 (MQ=255)
                                            aTCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCAtt                                                                                                   <  1:280135/149‑1 (MQ=255)
                                                 gCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCgg                                                                                              >  2:597149/1‑149 (MQ=255)
                                                      cgccgAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCa                                                                                                                                                <  4:92261/94‑1 (MQ=255)
                                                       gccgAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgcga                                                                                        >  3:1206278/1‑149 (MQ=255)
                                                                  ccAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCAt                                                                             >  2:560490/1‑149 (MQ=255)
                                                                   cAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATc                                                                            >  4:1080984/1‑149 (MQ=255)
                                                                   cAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATc                                                                            <  1:560490/149‑1 (MQ=255)
                                                                    aGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTGAAGCCTGGTTGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgcgatg                                                                                      <  4:1005394/138‑1 (MQ=255)
                                                                    aGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgcgatg                                                                                      >  3:1005394/1‑138 (MQ=255)
                                                                     gCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCtt                                                                          >  3:1159044/1‑149 (MQ=255)
                                                                         atTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCAt                                                                                                                                        >  4:479129/1‑83 (MQ=255)
                                                                         atTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCAt                                                                                                                                        <  3:479129/83‑1 (MQ=255)
                                                                          ttCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGt                                                                                                                  >  4:1394449/1‑104 (MQ=255)
                                                                          ttCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGt                                                                                                                  <  3:1394449/104‑1 (MQ=255)
                                                                          ttCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgcga                                                                                        >  1:15155/1‑130 (MQ=255)
                                                                          ttCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCg                                                                     >  1:971164/1‑149 (MQ=255)
                                                                            cgcgATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGc                                                                   <  4:1206278/149‑1 (MQ=255)
                                                                                    cATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACgg                                                             >  1:1429357/1‑147 (MQ=255)
                                                                                    cATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACgg                                                             <  2:1429357/147‑1 (MQ=255)
                                                                                        aGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATgg                                                                                                                           <  4:546779/81‑1 (MQ=255)
                                                                                        aGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATgg                                                                                                                           >  3:546779/1‑81 (MQ=255)
                                                                                         gcgcGGATGGGATTAAGTAAAGTCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGt                                                           <  3:1006647/144‑1 (MQ=255)
                                                                                         gcgcGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGt                                                           >  4:1006647/1‑144 (MQ=255)
                                                                                           gcgGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAAc                                                    <  2:971164/149‑1 (MQ=255)
                                                                                           gcgGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTACGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTATCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAAc                                                    <  4:913657/149‑1 (MQ=255)
                                                                                                tGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAgg                                               <  3:76409/149‑1 (MQ=255)
                                                                                                   gATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTa                                                 <  2:976673/144‑1 (MQ=255)
                                                                                                   gATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTa                                                 >  1:976673/1‑144 (MQ=255)
                                                                                                    aTTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAgg                                               <  2:878057/145‑1 (MQ=255)
                                                                                                    aTTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAgg                                               >  1:878057/1‑145 (MQ=255)
                                                                                                        aGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGc                                                         <  4:1266335/131‑1 (MQ=255)
                                                                                                        aGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGc                                                         >  3:1266335/1‑131 (MQ=255)
                                                                                                         gTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAg                                      <  1:597149/149‑1 (MQ=255)
                                                                                                         gTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAg                                      <  4:1159044/149‑1 (MQ=255)
                                                                                                          tAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCt                                                                 <  4:615877/121‑1 (MQ=255)
                                                                                                          tAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCt                                                                 >  3:615877/1‑121 (MQ=255)
                                                                                                          tAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAgg                                     <  3:167727/149‑1 (MQ=255)
                                                                                                              gCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgat                                                                                            >  1:1067713/1‑90 (MQ=255)
                                                                                                              gCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgat                                                                                            <  2:1067713/90‑1 (MQ=255)
                                                                                                              gCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGtt                                 >  2:1074775/1‑149 (MQ=255)
                                                                                                                             aCGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGc                                                         >  4:102762/1‑110 (MQ=255)
                                                                                                                             aCGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGc                                                         <  3:102762/110‑1 (MQ=255)
                                                                                                                                   gTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGTATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATaa                                       >  3:873093/1‑122 (MQ=255)
                                                                                                                                   gTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTc                                             >  3:867519/1‑116 (MQ=255)
                                                                                                                                   gTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTc                                             <  4:867519/116‑1 (MQ=255)
                                                                                                                                   gTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATaa                                       <  4:873093/122‑1 (MQ=255)
                                                                                                                                   gTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAg                                      <  1:605013/123‑1 (MQ=255)
                                                                                                                                   gTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAAt            >  4:357800/1‑149 (MQ=255)
                                                                                                                                   gTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATACGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAg                                      >  2:605013/1‑123 (MQ=255)
                                                                                                                                    tCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAg                                      <  4:904713/122‑1 (MQ=255)
                                                                                                                                    tCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAg                                      >  3:904713/1‑122 (MQ=255)
                                                                                                                                      aGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGc         <  3:357800/149‑1 (MQ=255)
                                                                                                                                       gATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGcc                                                        >  1:924687/1‑101 (MQ=255)
                                                                                                                                       gATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGcc                                                        <  2:924687/101‑1 (MQ=255)
                                                                                                                                       gATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGt                                  >  2:936779/1‑123 (MQ=255)
                                                                                                                                       gATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGt                                  <  1:936779/123‑1 (MQ=255)
                                                                                                                                        aTAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTa                                                                         >  4:72871/1‑83 (MQ=255)
                                                                                                                                        aTAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTa                                                                         <  3:72871/83‑1 (MQ=255)
                                                                                                                                          aaGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACa                 >  3:453067/1‑137 (MQ=255)
                                                                                                                                          aaGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACa                 <  4:453067/137‑1 (MQ=255)
                                                                                                                                            ggCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAAt            >  1:700544/1‑140 (MQ=255)
                                                                                                                                            ggCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAAt            <  2:700544/140‑1 (MQ=255)
                                                                                                                                             gCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATc                                                                            >  4:234796/1‑75 (MQ=255)
                                                                                                                                             gCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATc                                                                            <  3:234796/75‑1 (MQ=255)
                                                                                                                                             gCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGtttt  >  3:419292/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GGCAAAACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTT  >  NC_000913/3082563‑3082852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: