Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 276,935 C→A 5.5% T74K (ACG→AAG)  mmuM → CP4‑6 prophage; S‑methylmethionine:homocysteine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913276,9350CA 5.5% 326.7 / 5.5 91T74K (ACG→AAG) mmuMCP4‑6 prophage; S‑methylmethionine:homocysteine methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (43/43);  new base A (2/3);  total (45/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGT  >  NC_000913/276790‑277079
                                                                                                                                                 |                                                                                                                                                
gAGCTGGAAGCGCGAGGGTGAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCcg                                                                                                                                              >  3:221117/1‑149 (MQ=255)
     ggAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGAAGCccgccg                                                                                                                                          >  3:1423774/1‑147 (MQ=255)
      gAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCggg                                                                                                                                         >  2:1200386/1‑149 (MQ=255)
            cgAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGAAGCCCGCCGGCGTcgc                                                                                                                                   <  4:1423774/149‑1 (MQ=255)
                 gtgtAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGAAGCCCGCCGGCGTcgcctgat                                                                                                                              <  3:502411/149‑5 (MQ=255)
                   gtAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCcgcgc                                                                                                                              <  1:1089950/147‑1 (MQ=255)
                   gtAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCcgcgc                                                                                                                              >  2:1089950/1‑147 (MQ=255)
                     aaCTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAgg                                                                                                                          <  2:975469/149‑1 (MQ=255)
                       cTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTc                                                                                                                        >  3:377314/1‑149 (MQ=255)
                        ttAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCt                                                                                                                       >  2:362193/1‑149 (MQ=255)
                          aGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTgg                                                                                                                     >  2:845914/1‑149 (MQ=255)
                              gACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGt                                                                                                                         <  2:1544/141‑1 (MQ=255)
                              gACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGt                                                                                                                         >  1:1544/1‑141 (MQ=255)
                               aCAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGaa                                                                                                                >  1:1099173/1‑149 (MQ=255)
                                 aGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAgc                                                                                                              <  1:1200386/149‑1 (MQ=255)
                                   ccTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAgcgc                                                                                                            <  1:974352/149‑1 (MQ=255)
                                    cTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGc                                                                                                                                        <  3:989631/120‑1 (MQ=255)
                                    cTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGc                                                                                                                                        >  4:989631/1‑120 (MQ=255)
                                    cTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCa                                                                                                           <  4:221117/149‑1 (MQ=255)
                                         gTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGa                                                                                                                    >  2:650643/1‑135 (MQ=255)
                                         gTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGa                                                                                                                    <  1:650643/135‑1 (MQ=255)
                                              ccAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAgcg                                                                                                 >  1:323381/1‑149 (MQ=255)
                                               cAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCcgg                                                                                                                                             >  2:1338920/1‑104 (MQ=255)
                                               cAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCcgg                                                                                                                                             <  1:1338920/104‑1 (MQ=255)
                                               cAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAgcgc                                                                                                >  1:1413782/1‑149 (MQ=255)
                                               cAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAgcgc                                                                                                >  1:782784/1‑149 (MQ=255)
                                               cAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAgcgc                                                                                                >  1:24855/1‑149 (MQ=255)
                                                aaaGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGTGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGTAAGCGCt                                                                                               >  3:1259928/1‑149 (MQ=255)
                                                  aGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAg                                                                                                                           <  2:393032/119‑1 (MQ=255)
                                                  aGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAg                                                                                                                           >  1:393032/1‑119 (MQ=255)
                                                  aGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGa                                                                                             <  2:24855/149‑1 (MQ=255)
                                                  aGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGa                                                                                             <  2:1413782/149‑1 (MQ=255)
                                                    tGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGAtt                                                                                           <  4:377314/149‑1 (MQ=255)
                                                         gTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTcg                                                                                                                                    >  3:1277009/1‑103 (MQ=255)
                                                         gTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTcg                                                                                                                                    <  4:1277009/103‑1 (MQ=255)
                                                         gTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAg                                                                                                                           <  2:1052313/112‑1 (MQ=255)
                                                         gTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAg                                                                                                                           >  1:1052313/1‑112 (MQ=255)
                                                         gTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCaa                                                                                      >  2:983575/1‑149 (MQ=255)
                                                            gAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTc                                                                                                        <  3:739923/128‑1 (MQ=255)
                                                            gAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTc                                                                                                        >  4:739923/1‑128 (MQ=255)
                                                             aaaaCCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTgg                                                                                                                     >  4:36054/1‑114 (MQ=255)
                                                             aaaaCCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTgg                                                                                                                     <  3:36054/114‑1 (MQ=255)
                                                             aaaaCCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGa                                                                                                                    <  4:1353069/115‑1 (MQ=255)
                                                             aaaaCCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGa                                                                                                                    >  3:1353069/1‑115 (MQ=255)
                                                               aaCCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGt                                                                                <  2:782784/149‑1 (MQ=255)
                                                                aCCCGGAGCTTATCCGCGAAGCGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTg                                                                               <  2:323381/149‑1 (MQ=255)
                                                                  ccGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGa                                                                             >  3:785540/1‑149 (MQ=255)
                                                                  ccGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGa                                                                             >  4:374745/1‑149 (MQ=255)
                                                                       gCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCcggcg                                                                                                                                           >  2:362932/1‑82 (MQ=255)
                                                                       gCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCcggcg                                                                                                                                           <  1:362932/82‑1 (MQ=255)
                                                                         ttATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGgcg                                                                      >  1:1438076/1‑149 (MQ=255)
                                                                          tATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGgcgc                                                                     >  1:567122/1‑149 (MQ=255)
                                                                           aTCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGcgcg                                                                    >  3:414164/1‑149 (MQ=255)
                                                                                  aaGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGcc                                                             <  4:785540/149‑1 (MQ=255)
                                                                                  aaGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGcc                                                             <  1:362193/149‑1 (MQ=255)
                                                                                      gCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGt                                                                                >  4:991679/1‑126 (MQ=255)
                                                                                      gCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGATATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGt                                                                                <  3:991679/126‑1 (MQ=255)
                                                                                        aTCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGaa                                                       >  1:1276152/1‑149 (MQ=255)
                                                                                        aTCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGaa                                                       <  3:374745/149‑1 (MQ=255)
                                                                                         tCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAgc                                                                                                              <  1:229249/93‑1 (MQ=255)
                                                                                         tCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAgc                                                                                                              >  2:229249/1‑93 (MQ=255)
                                                                                         tCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAg                                                      <  2:1099173/149‑1 (MQ=255)
                                                                                           ttGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGt                                                                                                         >  2:1308211/1‑96 (MQ=255)
                                                                                           ttGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGt                                                                                                         <  1:1308211/96‑1 (MQ=255)
                                                                                               ttACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTg                                                                               >  2:65693/1‑118 (MQ=255)
                                                                                               ttACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTg                                                                               <  1:65693/118‑1 (MQ=255)
                                                                                                 actacCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTg                                              <  1:845914/149‑1 (MQ=255)
                                                                                                  ctacCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTgg                                             <  1:983575/149‑1 (MQ=255)
                                                                                                    acCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAGGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGaa                                                       <  4:857754/137‑1 (MQ=255)
                                                                                                    acCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTg                                                                         >  2:694777/1‑119 (MQ=255)
                                                                                                    acCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTg                                                                         <  1:694777/119‑1 (MQ=255)
                                                                                                    acCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGaa                                                       >  3:857754/1‑137 (MQ=255)
                                                                                                    acCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTa                                                  <  4:1171846/142‑1 (MQ=255)
                                                                                                    acCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTa                                                  >  3:1171846/1‑142 (MQ=255)
                                                                                                     ccGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGc                                                     <  4:1237501/138‑1 (MQ=255)
                                                                                                     ccGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGc                                                     >  3:1237501/1‑138 (MQ=255)
                                                                                                      cgggcgggGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCaga                                         <  2:1438076/149‑1 (MQ=255)
                                                                                                             gggCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTgg                                                                                                                     >  2:1204715/1‑66 (MQ=255)
                                                                                                             gggCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTgg                                                                                                                     <  1:1204715/66‑1 (MQ=255)
                                                                                                                    aTGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCgg                           <  4:414164/149‑1 (MQ=255)
                                                                                                                      gcgcGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGa                         <  2:1276152/149‑1 (MQ=255)
                                                                                                                       agcgATCACTGCCAGCTATCAGGCGACGGCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGAAGGCGGGaa                        <  4:1259928/148‑1 (MQ=255)
                                                                                                                                tGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGAtt                                                                                           >  2:1450422/1‑73 (MQ=255)
                                                                                                                                tGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGAtt                                                                                           <  1:1450422/73‑1 (MQ=255)
                                                                                                                                          gcggCGAAGCCCGCCGGCGTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGaa                                                       <  2:608923/97‑1 (MQ=255)
                                                                                                                                          gcggCGAAGCCCGCCGGCGTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGaa                                                       >  1:608923/3‑99 (MQ=255)
                                                                                                                                             gCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGt  >  4:583655/1‑149 (MQ=255)
                                                                                                                                               gACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCt                                                                          >  3:704315/1‑75 (MQ=255)
                                                                                                                                               gACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCt                                                                          <  4:704315/75‑1 (MQ=255)
                                                                                                                                                aCGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAg                                                                                                   <  2:491085/49‑1 (MQ=255)
                                                                                                                                                aCGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAg                                                                                                   >  1:491085/1‑49 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAGTCGAAAGCGCTGATTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGT  >  NC_000913/276790‑277079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: