Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,101,541 G→C 13.3% P28R (CCG→CGG)  yggN ← DUF2884 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,101,5410GC13.3% 496.0 / 57.1 157P28R (CCG→CGG) yggNDUF2884 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (62/74);  new base C (9/12);  total (71/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

TGATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAT  >  NC_000913/3101394‑3101670
                                                                                                                                                   |                                                                                                                                 
tGATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACgcgg                                                                                                                                  >  2:989112/1‑149 (MQ=255)
 gATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACgcggc                                                                                                                                 <  3:330182/149‑1 (MQ=255)
    aaTCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTg                                                                                                                              >  3:423401/1‑149 (MQ=255)
      tCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCCGCGTGAc                                                                                                                            >  4:737239/1‑149 (MQ=255)
        cTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCCGCGTGACga                                                                                                                          >  1:1352483/1‑148 (MQ=255)
         ttCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCt                                                                                                                         <  2:838678/149‑1 (MQ=255)
         ttCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACAGCGTCGCGCCGCGTGACgat                                                                                                                         <  1:1098036/149‑3 (MQ=255)
         ttCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCCGGCTGACAATCACATCGTCACGCGGCGTGACGCt                                                                                                                         >  1:296581/1‑149 (MQ=255)
            gCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCa                                                                                                                      <  1:1003971/149‑1 (MQ=255)
            gCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCa                                                                                                                      <  3:49147/149‑1 (MQ=255)
             ccTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCAc                                                                                                                     >  3:816604/1‑149 (MQ=255)
               tGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCCGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTg                                                                                                                   <  2:296581/149‑1 (MQ=255)
                   cgcgCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAg                                                                                                               <  2:335549/149‑1 (MQ=255)
                    gcgcTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCCGCGTGACgat                                                                                                                         >  4:467562/1‑136 (MQ=255)
                    gcgcTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCCGCGTGACgat                                                                                                                         <  3:467562/138‑3 (MQ=255)
                             ggcATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCAc                                                                                                                     <  2:1296476/133‑1 (MQ=255)
                                aTTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCgg                                                                                                  >  2:740826/1‑149 (MQ=255)
                                aTTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCATGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCATGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCgg                                                                                                  >  3:331757/1‑149 (MQ=255)
                                 ttCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGt                                                                                                 >  4:467935/1‑149 (MQ=255)
                                     gggAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCAtt                                                                                             >  1:87964/1‑149 (MQ=255)
                                      ggAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTg                                                                                            >  3:1408917/1‑149 (MQ=255)
                                      ggAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCCGCGTGACgat                                                                                                                         >  3:727002/1‑118 (MQ=255)
                                      ggAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCATGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTg                                                                                            <  4:331757/149‑1 (MQ=255)
                                       gAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGc                                                                                           <  4:423401/149‑1 (MQ=255)
                                        aaTATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGcc                                                                                          <  2:1142939/149‑1 (MQ=255)
                                        aaTATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGcc                                                                                          <  1:996111/149‑1 (MQ=255)
                                            ttGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCa                                                                                      >  3:439888/1‑149 (MQ=255)
                                             tGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCAc                                                                                     <  2:1095344/149‑1 (MQ=255)
                                             tGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCAc                                                                                     <  1:1392041/149‑1 (MQ=255)
                                               cTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTg                                                                                   <  1:740826/149‑1 (MQ=255)
                                                ttACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGa                                                                                  >  1:1215394/1‑149 (MQ=255)
                                                ttACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGa                                                                                  >  4:293050/1‑149 (MQ=255)
                                                ttACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGa                                                                                  <  4:1432983/149‑1 (MQ=255)
                                                ttACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGa                                                                                  >  4:1354854/1‑149 (MQ=255)
                                                  aCCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGaaa                                                                                <  3:551764/149‑1 (MQ=255)
                                                  aCCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGaaa                                                                                <  2:87964/149‑1 (MQ=255)
                                                  aCCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGaaa                                                                                <  1:3282/149‑1 (MQ=255)
                                                    cGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGt                                                                              >  1:41442/1‑149 (MQ=255)
                                                    cGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGt                                                                              >  2:1292650/1‑149 (MQ=255)
                                                      ttATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCAtt                                                                                             <  4:130485/132‑1 (MQ=255)
                                                      ttATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGtgc                                                                            >  3:690110/1‑149 (MQ=255)
                                                       tataCATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGtgct                                                                           <  4:66194/149‑1 (MQ=255)
                                                       tataCATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTACACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGtgct                                                                           <  3:54263/149‑1 (MQ=255)
                                                            aTCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTc                                                                                       <  2:1384779/132‑1 (MQ=255)
                                                            aTCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCa                                                                                      <  3:623783/133‑1 (MQ=255)
                                                            aTCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCa                                                                                      >  4:623783/1‑133 (MQ=255)
                                                            aTCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGtgct                                                                           <  4:687768/144‑1 (MQ=255)
                                                            aTCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTgccgc                                                                      <  3:293050/149‑1 (MQ=255)
                                                             tCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTgccgcc                                                                     >  3:731744/1‑149 (MQ=255)
                                                                 gTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCAt                                                                                              >  4:161511/1‑120 (MQ=255)
                                                                 gTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCAt                                                                                              <  3:161511/120‑1 (MQ=255)
                                                                  ttGCCGTCTGGCGTGATCACCAGATTGACGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTc                                                                                                >  3:1296625/1‑117 (MQ=255)
                                                                    gCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAgcagca                                                              <  1:1142113/149‑1 (MQ=255)
                                                                     ccGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCTCCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCTCTGGTAGTCGGCGTGAGCGGTCATTGCCGTCCCTGAAAGTGCTGCTGGCGgcagcac                                                             <  2:199505/149‑2 (MQ=255)
                                                                     ccGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCAc                                                                                                                     >  4:1441657/1‑93 (MQ=255)
                                                                       gTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCAc                                                                                                                     <  4:961562/91‑1 (MQ=255)
                                                                       gTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCAc                                                                                                                     <  3:1084487/91‑1 (MQ=255)
                                                                       gTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCAc                                                                                                                     >  4:1084487/1‑91 (MQ=255)
                                                                       gTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGt                                                                                                        >  1:1018136/1‑104 (MQ=255)
                                                                       gTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGt                                                                                                        <  2:1018136/104‑1 (MQ=255)
                                                                          tGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTgc                                                        <  3:467935/149‑1 (MQ=255)
                                                                            gCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAg                                                                               >  2:863476/1‑124 (MQ=255)
                                                                              gTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCCGCGTGACGATgtg                                                                                                                      <  1:584971/83‑3 (MQ=39)
                                                                              gTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCCGCGTGACGATgtg                                                                                                                      >  2:584971/1‑81 (MQ=255)
                                                                               tGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGcatc                                                   <  1:1292650/149‑1 (MQ=255)
                                                                                  tCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATa                                                <  4:581924/149‑1 (MQ=255)
                                                                                  tCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATa                                                <  1:902713/149‑1 (MQ=255)
                                                                                  tCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTCGCGCATCATa                                                <  3:90051/149‑1 (MQ=255)
                                                                                   cACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGc                                                                                                    >  4:494634/1‑96 (MQ=255)
                                                                                        gATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTc                                          >  3:624726/1‑149 (MQ=255)
                                                                                         aTTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTcc                                         <  4:731744/149‑1 (MQ=255)
                                                                                         aTTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTcc                                         <  2:1215394/149‑1 (MQ=255)
                                                                                             ccGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATAACATCGTCACCCGGCGTGACGCTGCACAGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCa                                     >  3:1399015/1‑149 (MQ=255)
                                                                                              cGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCaa                                    <  4:1399015/149‑1 (MQ=255)
                                                                                               gTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTc                                                                                                <  3:1000504/88‑1 (MQ=255)
                                                                                               gTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTACACTGGTAGTCGGCGTGAGCGGTc                                                                                                >  4:1000504/1‑88 (MQ=255)
                                                                                                     gCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATa                                                <  4:691309/130‑1 (MQ=255)
                                                                                                     gCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAg                                               >  1:593627/1‑131 (MQ=255)
                                                                                                     gCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAg                                               <  2:593627/131‑1 (MQ=255)
                                                                                                       ccTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATc                           >  1:841920/1‑149 (MQ=255)
                                                                                                       ccTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATc                           <  2:841920/149‑1 (MQ=255)
                                                                                                        cTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACt                                                                                    <  3:1267931/91‑1 (MQ=255)
                                                                                                        cTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACt                                                                                    >  4:1267931/1‑91 (MQ=255)
                                                                                                        cTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCa                          <  2:41442/149‑1 (MQ=255)
                                                                                                         ttCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAg                         <  4:439888/149‑1 (MQ=255)
                                                                                                                gCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGcat                                                    >  3:116590/1‑115 (MQ=255)
                                                                                                                gCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATa                                                >  4:878629/1‑119 (MQ=255)
                                                                                                                 cACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCa                          >  3:1325399/1‑140 (MQ=255)
                                                                                                                    ggTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCaaatg              <  1:844849/149‑5 (MQ=255)
                                                                                                                           gggCTGACAATCACATCGTCACGCGGCGTGACGCTGTACTGGTACTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCGCAAGAAAAAATCAGGCCAGCATTTGCTGGccc       >  3:1234219/1‑149 (MQ=255)
                                                                                                                             gCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCAttt                                                           <  3:1442927/95‑1 (MQ=255)
                                                                                                                             gCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTgg          >  2:175063/1‑144 (MQ=255)
                                                                                                                             gCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTgg          <  1:175063/144‑1 (MQ=255)
                                                                                                                                gACATTCACATCGTCACGCGGCGTGACGCTGCACTGGTATTCGGCGTCAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCACTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAt  <  4:1234219/149‑1 (MQ=255)
                                                                                                                                         aTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGaaa                                                                                <  1:757246/62‑1 (MQ=255)
                                                                                                                                         aTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGaaa                                                                                >  2:757246/1‑62 (MQ=255)
                                                                                                                                         aTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCAttt                                                           >  3:823513/1‑83 (MQ=255)
                                                                                                                                         aTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCAttt                                                           <  4:823513/83‑1 (MQ=255)
                                                                                                                                               aCGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTc                                          >  1:737229/1‑94 (MQ=255)
                                                                                                                                               aCGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTc                                          <  2:737229/94‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                 
TGATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAT  >  NC_000913/3101394‑3101670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: