Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,192,129 T→A 5.3% intergenic (‑190/‑79) glgS ← / → yqiJ motility and biofilm regulator/DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,192,1290TA 5.3% 275.7 / 5.2 75intergenic (‑190/‑79)glgS/yqiJmotility and biofilm regulator/DUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (37/34);  new base A (2/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

TTTTTCTGATTTCAGTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGT  >  NC_000913/3191983‑3192277
                                                                                                                                                  |                                                                                                                                                    
tttttCTGATTTCAGTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttatta                                                                                                                                                    >  4:1439223/1‑149 (MQ=255)
  tttCTGATTTCAGTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttattatt                                                                                                                                                  <  2:943430/149‑1 (MQ=255)
    tCTGATTTCAGTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                                                                                                                <  3:1439223/149‑1 (MQ=255)
                 ttCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGt                                                                                                                                   >  3:532955/1‑149 (MQ=255)
                 ttCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGt                                                                                                                                   >  2:1372338/1‑149 (MQ=255)
                  tCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTa                                                                                                                                  >  1:1438715/1‑149 (MQ=255)
                   ccccAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTaa                                                                                                                                 <  1:1372338/149‑1 (MQ=255)
                       aaTCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCaa                                                                                                                             <  4:532955/149‑1 (MQ=255)
                       aaTCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCaa                                                                                                                             <  2:1438715/149‑1 (MQ=255)
                                      tttAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTAATAATAACACAg                                                                                                                                           >  2:826324/1‑120 (MQ=255)
                                      tttAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTAATAATAACACAg                                                                                                                                           <  1:826324/120‑1 (MQ=255)
                                        tAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                              <  4:355237/147‑1 (MQ=255)
                                        tAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                              >  3:355237/1‑147 (MQ=255)
                                                    gCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACt                                                                                                                      <  4:1213197/127‑1 (MQ=255)
                                                    gCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACt                                                                                                                      >  3:1213197/1‑127 (MQ=255)
                                                                  tCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAg                                                                                                                            <  1:1015747/107‑1 (MQ=255)
                                                                  tCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAg                                                                                                                            >  2:1015747/1‑107 (MQ=255)
                                                                    cccGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGa                                                                                      <  2:1093670/143‑1 (MQ=255)
                                                                    cccGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGa                                                                                      >  1:1093670/1‑143 (MQ=255)
                                                                          cacaCTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttattatt                                                                                                                                                  <  2:1347359/77‑1 (MQ=255)
                                                                          cacaCTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttattatt                                                                                                                                                  >  1:1347359/1‑77 (MQ=255)
                                                                           acacTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttatta                                                                                                                                                    >  1:249214/1‑74 (MQ=255)
                                                                           acacTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttatta                                                                                                                                                    <  2:249214/74‑1 (MQ=255)
                                                                           acacTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAg                                                                                                                            >  2:957657/1‑98 (MQ=255)
                                                                           acacTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGat                                                                          >  1:1054736/1‑148 (MQ=255)
                                                                           acacTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGat                                                                          <  2:1054736/148‑1 (MQ=255)
                                                                           acacTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTATCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAg                                                                                                                            <  1:957657/98‑1 (MQ=255)
                                                                                          aaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAAt                                                                                                                                     >  4:693614/1‑74 (MQ=255)
                                                                                          aaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAAt                                                                                                                                     <  3:693614/74‑1 (MQ=255)
                                                                                          aaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAg                                                                                                                            <  4:993602/83‑1 (MQ=255)
                                                                                          aaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAg                                                                                                                            >  3:993602/1‑83 (MQ=255)
                                                                                             tAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGata                                                                         >  1:828659/1‑131 (MQ=255)
                                                                                             tAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGata                                                                         <  2:828659/131‑1 (MQ=255)
                                                                                                  aaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAg                                                                                                             <  4:823589/90‑1 (MQ=255)
                                                                                                  aaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAg                                                                                                             >  3:823589/1‑90 (MQ=255)
                                                                                                       tatTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACAcc                                             >  1:1224892/1‑149 (MQ=255)
                                                                                                         ttAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATGGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACAcccc                                           >  4:934941/1‑149 (MQ=255)
                                                                                                           aaaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGa                                                                           <  4:691533/115‑1 (MQ=255)
                                                                                                           aaaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGa                                                                           >  3:691533/1‑115 (MQ=255)
                                                                                                           aaaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTAtt                                                             <  2:805986/129‑1 (MQ=255)
                                                                                                           aaaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTAtt                                                             >  1:805986/1‑129 (MQ=255)
                                                                                                            aaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTa                                                                                                                  <  3:1305113/75‑1 (MQ=255)
                                                                                                            aaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTa                                                                                                                  >  4:1305113/1‑75 (MQ=255)
                                                                                                                  tttCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGAc                                                                                     >  4:1324311/1‑98 (MQ=255)
                                                                                                                  tttCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGAc                                                                                     <  3:1324311/98‑1 (MQ=255)
                                                                                                                  tttCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTAtt                                                             >  2:324944/1‑122 (MQ=255)
                                                                                                                  tttCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTAtt                                                             <  1:324944/122‑1 (MQ=255)
                                                                                                                  tttCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACta                                                    >  2:111087/1‑131 (MQ=255)
                                                                                                                  tttCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACta                                                    <  1:111087/131‑1 (MQ=255)
                                                                                                                    tctcTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTc                                 >  4:774745/1‑148 (MQ=255)
                                                                                                                    tctcTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTc                                 <  3:774745/148‑1 (MQ=255)
                                                                                                                          ccAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTa                                        <  1:318920/135‑1 (MQ=255)
                                                                                                                          ccAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTa                                        >  2:318920/1‑135 (MQ=255)
                                                                                                                           cAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGc                                                          >  4:788599/1‑116 (MQ=255)
                                                                                                                           cAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGc                                                          <  3:788599/116‑1 (MQ=255)
                                                                                                                            aGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGAt                                                                   <  2:706837/106‑1 (MQ=255)
                                                                                                                            aGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGAt                                                                   >  1:706837/1‑106 (MQ=255)
                                                                                                                                cTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                              >  3:652448/1‑59 (MQ=255)
                                                                                                                                cTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                              <  4:652448/59‑1 (MQ=255)
                                                                                                                                 ttCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGt                   >  2:134009/1‑149 (MQ=255)
                                                                                                                                   ctctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTc                                 <  1:706514/133‑1 (MQ=255)
                                                                                                                                   ctctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTc                                 >  4:80844/1‑133 (MQ=255)
                                                                                                                                   ctctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTc                                 >  2:706514/1‑133 (MQ=255)
                                                                                                                                   ctctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTc                                 <  3:80844/133‑1 (MQ=255)
                                                                                                                                    tctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                              <  1:297403/55‑1 (MQ=255)
                                                                                                                                    tctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                              >  2:297403/1‑55 (MQ=255)
                                                                                                                                    tctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACt                >  4:1439703/1‑149 (MQ=255)
                                                                                                                                      tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCg                                                                                                  <  2:1358818/65‑1 (MQ=255)
                                                                                                                                      tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCg                                                                                                  >  1:1358818/1‑65 (MQ=255)
                                                                                                                                      tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATaa                                                 <  3:276892/114‑1 (MQ=255)
                                                                                                                                      tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATaa                                                 >  4:276892/1‑114 (MQ=255)
                                                                                                                                        atcTGTGTTAATAATAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACAcc                                             >  2:933106/3‑116 (MQ=255)
                                                                                                                                        atcTGTGTTAATAATAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACAcc                                             <  1:933106/114‑1 (MQ=255)
                                                                                                                                          cTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGa           <  3:828966/148‑1 (MQ=255)
                                                                                                                                          cTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGa           >  4:828966/1‑148 (MQ=255)
                                                                                                                                           tgtgTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGatatat                                                                      >  1:1325458/1‑88 (MQ=255)
                                                                                                                                           tgtgTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGatatat                                                                      <  2:1325458/88‑1 (MQ=255)
                                                                                                                                           tgtgTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATc         >  2:1073441/1‑149 (MQ=255)
                                                                                                                                            gtgtTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCg        <  1:134009/149‑1 (MQ=255)
                                                                                                                                             tgtTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGa                                                                    >  4:517358/1‑88 (MQ=255)
                                                                                                                                             tgtTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGa                                                                    <  3:517358/88‑1 (MQ=255)
                                                                                                                                                  ttattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGt  <  4:799253/149‑1 (MQ=255)
                                                                                                                                                  ttattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGt  >  3:799253/1‑149 (MQ=255)
                                                                                                                                                  ttattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGt  >  4:1413105/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTTTTCTGATTTCAGTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGT  >  NC_000913/3191983‑3192277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: