Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,208,205 G→A 8.9% G61D (GGT→GAT)  ttdT → L‑tartrate/succinate antiporte

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,208,2050GA 8.9% 278.9 / 15.0 90G61D (GGT→GAT) ttdTL‑tartrate/succinate antiporte
Reads supporting (aligned to +/- strand):  ref base G (44/38);  new base A (3/5);  total (47/43)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

CGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGC  >  NC_000913/3208058‑3208349
                                                                                                                                                   |                                                                                                                                                
cgccgcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGt                                                                                                                                                 <  3:68009/149‑1 (MQ=255)
    gcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTatat                                                                                                                                             <  3:1299957/149‑1 (MQ=255)
           gTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCcaccat                                                                                                                                      <  2:1273951/149‑1 (MQ=255)
           gTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCcaccat                                                                                                                                      <  4:299125/149‑1 (MQ=255)
            tCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCatcatc                                                                                                                                     <  1:1004646/149‑1 (MQ=255)
              aTCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGc                                                                                                                                   >  2:1103938/1‑149 (MQ=255)
              aTCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGc                                                                                                                                   >  1:34111/1‑149 (MQ=255)
                  ccATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTggg                                                                                                                                                  <  2:650699/130‑1 (MQ=255)
                  ccATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTggg                                                                                                                                                  >  1:650699/1‑130 (MQ=255)
                  ccATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATa                                                                                                                               >  1:1100734/1‑149 (MQ=255)
                  ccATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATa                                                                                                                               <  2:34111/149‑1 (MQ=255)
                        ttGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATGCCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCatcatc                                                                                                                                     <  1:591638/137‑1 (MQ=255)
                        ttGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGACCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCatcatc                                                                                                                                     >  2:591638/1‑137 (MQ=255)
                        ttGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCcat                                                                                                                                         <  2:892443/133‑1 (MQ=255)
                        ttGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCcat                                                                                                                                         >  1:892443/1‑133 (MQ=255)
                           ctctACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTccc                                                                                                                      >  4:1412395/1‑149 (MQ=255)
                            tctACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCt                                                                                                                     >  1:1076202/1‑149 (MQ=255)
                            tctACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCt                                                                                                                     <  2:1076202/149‑1 (MQ=255)
                                 ttCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATa                                                                                                                               >  2:1434249/1‑134 (MQ=255)
                                 ttCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATa                                                                                                                               <  1:1434249/134‑1 (MQ=255)
                                 ttCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgctg                                                                                                                >  2:934327/1‑149 (MQ=255)
                                   ccGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgctgct                                                                                                              >  1:442673/1‑149 (MQ=255)
                                    cGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGta                                                                                                                                                <  2:150777/114‑1 (MQ=255)
                                    cGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGta                                                                                                                                                <  4:1411652/114‑1 (MQ=255)
                                    cGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGta                                                                                                                                                >  1:150777/1‑114 (MQ=255)
                                    cGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGta                                                                                                                                                >  3:1411652/1‑114 (MQ=255)
                                     ggTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGt                                                                                                            >  3:518566/1‑149 (MQ=255)
                                      gTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTGCTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGtt                                                                                                           >  4:1193680/1‑149 (MQ=255)
                                      gTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGta                                                                                                                                                <  3:1173820/112‑1 (MQ=255)
                                      gTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGta                                                                                                                                                >  4:1173820/1‑112 (MQ=255)
                                        tCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCa                                                                                                         <  2:1100734/149‑1 (MQ=255)
                                            gCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGccc                                                                                                     >  1:1015018/1‑149 (MQ=255)
                                                   tGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagcag                                                                                              <  3:1412395/149‑1 (MQ=255)
                                                    ggAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagcagc                                                                                             <  2:442673/149‑1 (MQ=255)
                                                          ccATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAgctcgctc                                                                                       >  2:713296/1‑149 (MQ=255)
                                                           cATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCCaccatc                                                                                                                                     <  2:464696/102‑1 (MQ=39)
                                                           cATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCCaccatc                                                                                                                                     >  1:464696/1‑102 (MQ=39)
                                                           cATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCCaccatc                                                                                                                                     <  2:808212/102‑1 (MQ=39)
                                                           cATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCCaccatc                                                                                                                                     >  1:808212/1‑102 (MQ=38)
                                                             tACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGc                                                                                    >  3:118952/1‑149 (MQ=255)
                                                                  ggCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGc                                                                               <  1:934327/149‑1 (MQ=255)
                                                                    ctctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCtt                                                                             <  2:1015018/149‑1 (MQ=255)
                                                                     tctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgct                                                                                                                 >  2:1101847/1‑112 (MQ=255)
                                                                     tctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgct                                                                                                                 <  1:1101847/112‑1 (MQ=255)
                                                                        aCTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                         <  1:1103938/149‑1 (MQ=255)
                                                                          tttGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGc                                                                                                                                   <  2:810962/89‑1 (MQ=255)
                                                                          tttGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGc                                                                                                                                   >  1:810962/1‑89 (MQ=255)
                                                                               cGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCAc                                                                    <  2:1087579/147‑1 (MQ=255)
                                                                               cGTTTTTACTGCCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCAc                                                                    >  1:1087579/1‑147 (MQ=255)
                                                                                gTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                         >  2:66880/1‑141 (MQ=255)
                                                                                gTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                         <  1:66880/141‑1 (MQ=255)
                                                                                 tttttACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCa                                                                >  3:17099/1‑149 (MQ=255)
                                                                                           cGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCctct                                                      <  4:118952/149‑1 (MQ=255)
                                                                                            gTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagcagc                                                                                             >  1:1194545/1‑109 (MQ=255)
                                                                                            gTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagcagc                                                                                             <  2:1194545/109‑1 (MQ=255)
                                                                                               aTCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTg                                                  >  4:525720/1‑149 (MQ=255)
                                                                                               aTCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTg                                                  >  3:1437601/1‑149 (MQ=255)
                                                                                               aTCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTg                                                  >  3:367044/1‑149 (MQ=255)
                                                                                                  gTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGc                                               >  1:354991/1‑149 (MQ=255)
                                                                                                     ggACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGt                                            >  3:132778/1‑149 (MQ=255)
                                                                                                      gACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGa                                                                                                  >  3:985299/1‑94 (MQ=255)
                                                                                                      gACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGa                                                                                                  <  4:985299/94‑1 (MQ=255)
                                                                                                      gACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGtt                                           <  4:17099/149‑1 (MQ=255)
                                                                                                        cTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTc                                         >  2:6941/1‑149 (MQ=255)
                                                                                                         tGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTcc                                        <  4:518566/149‑1 (MQ=255)
                                                                                                          gATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgct                                                                                                                 <  1:1053670/75‑1 (MQ=255)
                                                                                                          gATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgct                                                                                                                 >  2:1053670/1‑75 (MQ=255)
                                                                                                          gATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCg                                       <  1:713296/149‑1 (MQ=255)
                                                                                                           aTCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCAc                                                                    <  4:1072154/119‑1 (MQ=255)
                                                                                                           aTCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCAc                                                                    >  3:1072154/1‑119 (MQ=255)
                                                                                                                 gAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTc                                <  3:1193680/149‑1 (MQ=255)
                                                                                                                   aCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTa                              >  3:1305871/1‑149 (MQ=255)
                                                                                                                     ccGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCa                                                                     >  2:643721/1‑108 (MQ=255)
                                                                                                                     ccGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCa                                                                     <  1:643721/108‑1 (MQ=255)
                                                                                                                        tGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCg                         <  4:132778/149‑1 (MQ=255)
                                                                                                                          ccGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATActc                                                                                                                            <  1:1251850/48‑1 (MQ=255)
                                                                                                                          ccGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATActc                                                                                                                            >  2:1251850/1‑48 (MQ=255)
                                                                                                                              gTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTATTCAGCCGGGAGCAGCTCGCTCAGCCAGTCTTTAAATTCGCTGCAAAATGCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATc                   >  3:911036/1‑149 (MQ=255)
                                                                                                                                   gtggtgGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCt              <  4:367044/149‑1 (MQ=255)
                                                                                                                                   gtggtgGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCt              >  1:526093/1‑149 (MQ=255)
                                                                                                                                    tggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATc                                                           >  2:1231256/1‑103 (MQ=255)
                                                                                                                                    tggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATc                                                           <  1:1231256/103‑1 (MQ=255)
                                                                                                                                    tggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGt                                     <  2:585821/125‑1 (MQ=255)
                                                                                                                                    tggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGt                                     >  1:585821/1‑125 (MQ=255)
                                                                                                                                     ggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTgg                <  4:939332/145‑1 (MQ=255)
                                                                                                                                     ggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTgg                >  3:939332/1‑145 (MQ=255)
                                                                                                                                           gatgatGGATATACCCACCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                         <  4:1228460/82‑1 (MQ=38)
                                                                                                                                           gatgatGGATATACCCACCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                         >  3:1228460/1‑82 (MQ=38)
                                                                                                                                           gATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGcc                                                                                                      <  1:589984/53‑1 (MQ=255)
                                                                                                                                           gATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGcc                                                                                                      >  2:589984/1‑53 (MQ=255)
                                                                                                                                           gATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTcg      >  3:271838/1‑149 (MQ=255)
                                                                                                                                               gtggGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTcgccgc  >  1:618964/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGC  >  NC_000913/3208058‑3208349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: