Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,208,210 T→C 9.7% S63P (TCC→CCC)  ttdT → L‑tartrate/succinate antiporte

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,208,2100TC 9.7% 252.3 / 15.1 82S63P (TCC→CCC) ttdTL‑tartrate/succinate antiporte
Reads supporting (aligned to +/- strand):  ref base T (41/33);  new base C (3/5);  total (44/38)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

GCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTT  >  NC_000913/3208062‑3208358
                                                                                                                                                    |                                                                                                                                                    
gcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTatat                                                                                                                                                      <  3:1299957/149‑1 (MQ=255)
       gTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCcaccat                                                                                                                                               <  4:299125/149‑1 (MQ=255)
       gTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCcaccat                                                                                                                                               <  2:1273951/149‑1 (MQ=255)
        tCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCatcatc                                                                                                                                              <  1:1004646/149‑1 (MQ=255)
          aTCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGc                                                                                                                                            >  2:1103938/1‑149 (MQ=255)
          aTCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGc                                                                                                                                            >  1:34111/1‑149 (MQ=255)
              ccATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATa                                                                                                                                        >  1:1100734/1‑149 (MQ=255)
              ccATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATa                                                                                                                                        <  2:34111/149‑1 (MQ=255)
                    ttGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATGCCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCatcatc                                                                                                                                              <  1:591638/137‑1 (MQ=255)
                    ttGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGACCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCatcatc                                                                                                                                              >  2:591638/1‑137 (MQ=255)
                    ttGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCcat                                                                                                                                                  >  1:892443/1‑133 (MQ=255)
                    ttGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCcat                                                                                                                                                  <  2:892443/133‑1 (MQ=255)
                       ctctACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTccc                                                                                                                               >  4:1412395/1‑149 (MQ=255)
                        tctACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCt                                                                                                                              >  1:1076202/1‑149 (MQ=255)
                        tctACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCt                                                                                                                              <  2:1076202/149‑1 (MQ=255)
                             ttCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATa                                                                                                                                        <  1:1434249/134‑1 (MQ=255)
                             ttCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATa                                                                                                                                        >  2:1434249/1‑134 (MQ=255)
                             ttCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgctg                                                                                                                         >  2:934327/1‑149 (MQ=255)
                               ccGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgctgct                                                                                                                       >  1:442673/1‑149 (MQ=255)
                                 ggTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGt                                                                                                                     >  3:518566/1‑149 (MQ=255)
                                  gTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTGCTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGtt                                                                                                                    >  4:1193680/1‑149 (MQ=255)
                                    tCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCa                                                                                                                  <  2:1100734/149‑1 (MQ=255)
                                        gCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGccc                                                                                                              >  1:1015018/1‑149 (MQ=255)
                                               tGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagcag                                                                                                       <  3:1412395/149‑1 (MQ=255)
                                                ggAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagcagc                                                                                                      <  2:442673/149‑1 (MQ=255)
                                                      ccATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAgctcgctc                                                                                                >  2:713296/1‑149 (MQ=255)
                                                       cATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCCaccatc                                                                                                                                              <  2:808212/102‑1 (MQ=39)
                                                       cATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCCaccatc                                                                                                                                              >  1:464696/1‑102 (MQ=39)
                                                       cATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCCaccatc                                                                                                                                              <  2:464696/102‑1 (MQ=39)
                                                       cATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCCaccatc                                                                                                                                              >  1:808212/1‑102 (MQ=38)
                                                         tACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGc                                                                                             >  3:118952/1‑149 (MQ=255)
                                                              ggCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGc                                                                                        <  1:934327/149‑1 (MQ=255)
                                                                ctctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCtt                                                                                      <  2:1015018/149‑1 (MQ=255)
                                                                 tctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgct                                                                                                                          <  1:1101847/112‑1 (MQ=255)
                                                                 tctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgct                                                                                                                          >  2:1101847/1‑112 (MQ=255)
                                                                    aCTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                                  <  1:1103938/149‑1 (MQ=255)
                                                                      tttGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGc                                                                                                                                            <  2:810962/89‑1 (MQ=255)
                                                                      tttGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGc                                                                                                                                            >  1:810962/1‑89 (MQ=255)
                                                                           cGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCAc                                                                             <  2:1087579/147‑1 (MQ=255)
                                                                           cGTTTTTACTGCCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCAc                                                                             >  1:1087579/1‑147 (MQ=255)
                                                                            gTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                                  >  2:66880/1‑141 (MQ=255)
                                                                            gTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                                  <  1:66880/141‑1 (MQ=255)
                                                                             tttttACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCa                                                                         >  3:17099/1‑149 (MQ=255)
                                                                                       cGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCctct                                                               <  4:118952/149‑1 (MQ=255)
                                                                                        gTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagcagc                                                                                                      >  1:1194545/1‑109 (MQ=255)
                                                                                        gTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagcagc                                                                                                      <  2:1194545/109‑1 (MQ=255)
                                                                                           aTCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTg                                                           >  3:1437601/1‑149 (MQ=255)
                                                                                           aTCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTg                                                           >  3:367044/1‑149 (MQ=255)
                                                                                           aTCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTg                                                           >  4:525720/1‑149 (MQ=255)
                                                                                              gTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGc                                                        >  1:354991/1‑149 (MQ=255)
                                                                                                 ggACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGt                                                     >  3:132778/1‑149 (MQ=255)
                                                                                                  gACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGa                                                                                                           >  3:985299/1‑94 (MQ=255)
                                                                                                  gACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGa                                                                                                           <  4:985299/94‑1 (MQ=255)
                                                                                                  gACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGtt                                                    <  4:17099/149‑1 (MQ=255)
                                                                                                    cTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTc                                                  >  2:6941/1‑149 (MQ=255)
                                                                                                     tGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTcc                                                 <  4:518566/149‑1 (MQ=255)
                                                                                                      gATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgct                                                                                                                          <  1:1053670/75‑1 (MQ=255)
                                                                                                      gATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgct                                                                                                                          >  2:1053670/1‑75 (MQ=255)
                                                                                                      gATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCg                                                <  1:713296/149‑1 (MQ=255)
                                                                                                       aTCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCAc                                                                             >  3:1072154/1‑119 (MQ=255)
                                                                                                       aTCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCAc                                                                             <  4:1072154/119‑1 (MQ=255)
                                                                                                             gAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTc                                         <  3:1193680/149‑1 (MQ=255)
                                                                                                               aCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTa                                       >  3:1305871/1‑149 (MQ=255)
                                                                                                                 ccGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCa                                                                              >  2:643721/1‑108 (MQ=255)
                                                                                                                 ccGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCa                                                                              <  1:643721/108‑1 (MQ=255)
                                                                                                                    tGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCg                                  <  4:132778/149‑1 (MQ=255)
                                                                                                                      ccGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATActc                                                                                                                                     <  1:1251850/48‑1 (MQ=255)
                                                                                                                      ccGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATActc                                                                                                                                     >  2:1251850/1‑48 (MQ=255)
                                                                                                                          gTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTATTCAGCCGGGAGCAGCTCGCTCAGCCAGTCTTTAAATTCGCTGCAAAATGCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATc                            >  3:911036/1‑149 (MQ=255)
                                                                                                                               gtggtgGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCt                       <  4:367044/149‑1 (MQ=255)
                                                                                                                               gtggtgGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCt                       >  1:526093/1‑149 (MQ=255)
                                                                                                                                tggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATc                                                                    <  1:1231256/103‑1 (MQ=255)
                                                                                                                                tggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATc                                                                    >  2:1231256/1‑103 (MQ=255)
                                                                                                                                tggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGt                                              >  1:585821/1‑125 (MQ=255)
                                                                                                                                tggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGt                                              <  2:585821/125‑1 (MQ=255)
                                                                                                                                 ggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTgg                         <  4:939332/145‑1 (MQ=255)
                                                                                                                                 ggtggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTgg                         >  3:939332/1‑145 (MQ=255)
                                                                                                                                       gatgatGGATATACCCACCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                                  <  4:1228460/82‑1 (MQ=38)
                                                                                                                                       gatgatGGATATACCCACCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                                  >  3:1228460/1‑82 (MQ=38)
                                                                                                                                       gATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGcc                                                                                                               <  1:589984/53‑1 (MQ=255)
                                                                                                                                       gATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGcc                                                                                                               >  2:589984/1‑53 (MQ=255)
                                                                                                                                       gATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTcg               >  3:271838/1‑149 (MQ=255)
                                                                                                                                           gtggGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTcgccgc           >  1:618964/1‑149 (MQ=255)
                                                                                                                                                 atatCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCa                                                                              <  4:1275224/76‑1 (MQ=255)
                                                                                                                                                 atatCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCa                                                                              >  3:1275224/1‑76 (MQ=255)
                                                                                                                                                    tCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAgctcgc                                                                                                  >  2:646310/1‑53 (MQ=255)
                                                                                                                                                    tCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAgctcgc                                                                                                  <  1:646310/53‑1 (MQ=255)
                                                                                                                                                    tCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGtt                               >  4:198654/1‑120 (MQ=255)
                                                                                                                                                    tCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGtt                               <  3:198654/120‑1 (MQ=255)
                                                                                                                                                    tCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGAtt                   >  3:625584/1‑132 (MQ=255)
                                                                                                                                                    tCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGAtt                   <  4:625584/132‑1 (MQ=255)
                                                                                                                                                    tCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGtt  >  3:409198/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTT  >  NC_000913/3208062‑3208358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: