| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,234,506 | G→A | 16.5% | intergenic (‑51/+233) | higB ← / ← rlmG | mRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,234,506 | 0 | G | A | 16.5% | 154.7 / 20.2 | 64 | intergenic (‑51/+233) | higB/rlmG | mRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (26/25); new base A (5/5); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGA > NC_000913/3234367‑3234648 | aTCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCaa > 1:1276449/1‑148 (MQ=255)aTCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCaa < 2:1276449/148‑1 (MQ=255) tCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAATC‑TGGCAACg < 2:170455/149‑1 (MQ=255) accaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCaa > 3:1194384/1‑130 (MQ=255) accaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCaa < 4:1194384/130‑1 (MQ=255) ccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAATC‑TGGCAACgg > 3:387203/1‑132 (MQ=255) ccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAATC‑TGGCAACgg < 4:387203/132‑1 (MQ=255) cGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGgaag > 2:53942/1‑149 (MQ=255) ttttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑Tgg > 3:1218148/1‑117 (MQ=255) ttttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑Tgg < 4:1218148/117‑1 (MQ=255) ttttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCtgt > 2:1420540/1‑137 (MQ=255) ttttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCtgt < 1:1420540/137‑1 (MQ=255) ttGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAt < 4:402054/122‑1 (MQ=255) ttGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAt > 3:402054/1‑122 (MQ=255) agcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAttt > 3:548937/1‑106 (MQ=255) agcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAttt < 4:548937/106‑1 (MQ=255) agcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGgaa > 2:133164/1‑121 (MQ=255) agcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGgaa < 1:133164/121‑1 (MQ=255) gcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga < 1:53942/149‑1 (MQ=255) tGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAATC‑TGGCAACGGa < 1:546446/87‑1 (MQ=39) tGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCA‑ATCTGGCAACGGa > 2:546446/1‑87 (MQ=38) ttATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAAc < 1:528304/116‑1 (MQ=255) ttATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAAc > 2:528304/1‑116 (MQ=255) tGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAAc > 1:268799/1‑65 (MQ=255) tGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAAc < 2:268799/65‑1 (MQ=255) tGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATa < 4:1182878/95‑1 (MQ=255) tGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATa > 3:1182878/1‑95 (MQ=255) tGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAAc > 4:482220/1‑98 (MQ=255) tGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAAc < 3:482220/98‑1 (MQ=255) catcaCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTa < 2:184460/117‑1 (MQ=255) catcaCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTa > 1:184460/1‑117 (MQ=255) tcaCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGaa > 3:624289/1‑149 (MQ=255) gTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTAt > 2:129050/1‑62 (MQ=255) gTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTAt < 1:129050/62‑1 (MQ=255) gTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGAtt < 4:624289/149‑1 (MQ=255) aTTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACt > 4:369222/1‑149 (MQ=255) aCATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAAt < 4:1407014/81‑1 (MQ=255) aCATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAAt > 3:1407014/1‑81 (MQ=255) aTCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGttt > 2:632848/1‑109 (MQ=255) aTCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGttt < 1:632848/109‑1 (MQ=255) tCCGTTGCCAATC‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtttt < 1:1075672/109‑1 (MQ=255) tCCGTTGCCAATC‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtttt < 2:448789/109‑1 (MQ=255) tCCGTTGCCAATC‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtttt > 2:1075672/1‑109 (MQ=255) tCCGTTGCCAATC‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtttt > 1:448789/1‑109 (MQ=255) tCCGTTGCCAATC‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACAt > 2:621787/1‑141 (MQ=255) tCCGTTGCCA‑ATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACAt < 1:621787/141‑1 (MQ=255) ccGTTGCCAATC‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGAtt > 3:491726/1‑122 (MQ=255) ccGTTGCCA‑ATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGAtt < 4:491726/122‑1 (MQ=255) cGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGCTTTATGGAAGCGCGa > 4:501443/1‑119 (MQ=255) cGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGCTTTATGGAAGCGCGa < 3:501443/119‑1 (MQ=255) tGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaaaa < 2:461973/57‑1 (MQ=255) tGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaaaa > 1:461973/1‑57 (MQ=255) ccAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATg > 3:125218/1‑81 (MQ=255) ccAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATg < 4:125218/81‑1 (MQ=255) ccAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt < 3:706670/104‑1 (MQ=255) ccAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt > 2:485863/1‑104 (MQ=255) ccAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt < 1:485863/104‑1 (MQ=255) ccAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt > 4:706670/1‑104 (MQ=255) aGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGt > 3:601051/1‑97 (MQ=255) aGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGt < 4:601051/97‑1 (MQ=255) aGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt > 4:337317/1‑102 (MQ=255) aGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt < 3:337317/102‑1 (MQ=255) aGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGa < 4:1279411/144‑1 (MQ=255) aGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGa > 3:1279411/1‑144 (MQ=255) | ATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGA > NC_000913/3234367‑3234648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |