Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,256,443 | C→A | 7.4% | G70V (GGC→GTC) ‡ | yhaM ← | putative L‑serine dehydratase alpha chain |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,256,443 | 0 | C | A | 7.4% | 376.0 / 13.5 | 107 | G70V (GGC→GTC) ‡ | yhaM | putative L‑serine dehydratase alpha chain |
Reads supporting (aligned to +/- strand): ref base C (46/53); new base A (4/4); total (50/57) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 109 total aligned reads displayed.
GGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTAC > NC_000913/3256301‑3256591 | ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGaa < 1:318026/149‑1 (MQ=255) gagaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAAc > 4:1001350/1‑149 (MQ=255) agaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGCA‑CGGGaatg < 4:208248/149‑3 (MQ=255) aCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGt < 3:1345411/149‑1 (MQ=255) cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGcc > 1:1010419/1‑149 (MQ=255) ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGccc > 1:294374/1‑149 (MQ=255) ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCG‑GCACGGGAATGGTggg < 1:1388419/145‑3 (MQ=255) ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCG‑GCACGGGAATGGTggg > 2:1388419/1‑143 (MQ=255) ccgccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGa < 4:880231/149‑1 (MQ=255) gccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGAcc > 3:421121/1‑149 (MQ=255) agcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGtt < 2:1010419/149‑1 (MQ=255) gcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc > 3:1066972/1‑149 (MQ=255) gcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc > 1:589025/1‑149 (MQ=255) agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttctt < 3:901697/149‑1 (MQ=255) gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttcttc < 1:166465/149‑1 (MQ=255) gCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTtcat < 4:1066972/149‑1 (MQ=255) cTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGAc < 4:447210/130‑1 (MQ=255) cTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGAc > 3:447210/1‑130 (MQ=255) tttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTtcatca < 2:589025/149‑1 (MQ=255) tttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTtcatca < 4:648332/149‑1 (MQ=255) cATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAttt < 1:542611/149‑1 (MQ=255) aTCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGtt < 1:739552/135‑1 (MQ=255) aTCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGtt > 2:739552/1‑135 (MQ=255) aTCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTg > 4:694164/1‑149 (MQ=255) tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAAc > 3:124473/1‑116 (MQ=255) tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGa < 1:182097/129‑1 (MQ=255) tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGa > 2:182097/1‑129 (MQ=255) tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTgg > 4:501599/1‑149 (MQ=255) cGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGc < 3:1297923/149‑1 (MQ=255) aTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAAc < 3:501599/149‑1 (MQ=255) cTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAgg < 3:809179/149‑1 (MQ=255) tGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGc > 4:57168/1‑149 (MQ=255) tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttcttc > 1:1279464/1‑121 (MQ=255) tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttcttc < 2:1279464/121‑1 (MQ=255) tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTtcatca < 2:638531/125‑1 (MQ=255) tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTtcatca > 1:638531/1‑125 (MQ=255) cGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAAccc > 2:680513/1‑135 (MQ=255) cGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAAccc < 1:680513/135‑1 (MQ=255) cGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGt < 3:57168/149‑1 (MQ=255) gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTCCTACACGtt < 2:294374/149‑1 (MQ=255) tCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc < 3:1001350/149‑1 (MQ=255) gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttcttc < 3:875440/107‑1 (MQ=255) gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttcttc > 4:875440/1‑107 (MQ=255) ttCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCA‑CGGGaatg > 3:847441/1‑80 (MQ=38) ttCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCA‑CGGGaatg < 4:847441/82‑3 (MQ=38) ccAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAgg < 4:1254396/123‑1 (MQ=255) ccAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAgg > 3:1254396/1‑123 (MQ=255) ccGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc < 2:441732/93‑1 (MQ=255) ccGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc > 1:441732/1‑93 (MQ=255) ttGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgc > 4:1398927/1‑149 (MQ=255) gCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCGCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCa > 4:1243168/1‑136 (MQ=255) gCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCa < 3:1243168/136‑1 (MQ=255) aTTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc > 2:930480/1‑82 (MQ=255) aTTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc < 1:930480/82‑1 (MQ=255) aTTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCg > 1:127009/1‑125 (MQ=255) aTTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCg < 2:127009/125‑1 (MQ=255) tttCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAcc < 2:1314770/123‑1 (MQ=255) tttCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAcc > 1:1314770/1‑123 (MQ=255) tCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc > 1:845372/1‑79 (MQ=255) tCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc < 2:845372/79‑1 (MQ=255) cACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAc > 1:671754/1‑118 (MQ=255) cACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAc < 2:671754/118‑1 (MQ=255) cccAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCA‑CGGGAATGGTg < 4:204524/51‑1 (MQ=17) cccAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCA‑CGGGAATGGTg > 3:204524/1‑51 (MQ=12) aGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAc < 4:1300758/109‑1 (MQ=255) aGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAc > 3:1300758/1‑109 (MQ=255) gccgcAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAAt < 3:1398927/149‑1 (MQ=255) gccgcAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAAt < 3:694164/149‑1 (MQ=255) cAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGt < 1:230087/149‑1 (MQ=255) ggCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAAt > 2:14364/1‑140 (MQ=255) ggCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAAt < 1:14364/140‑1 (MQ=255) ccccACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCg > 4:847627/1‑137 (MQ=255) ccccACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCg < 3:847627/137‑1 (MQ=255) cccACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTc > 4:1371271/1‑140 (MQ=255) tcaccgTTCCCGGCA‑CGGGAATGGTGGCGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTc > 2:739104/2‑97 (MQ=38) ccaccaTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgc < 3:226376/115‑1 (MQ=255) ccaccaTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgc > 4:226376/1‑115 (MQ=255) ccaccaTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATccc > 3:714497/1‑149 (MQ=255) caccgTTCCCGGCA‑CGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCa > 4:400619/1‑92 (MQ=38) caccgTTCCCGGCA‑CGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCa < 3:400619/92‑1 (MQ=38) cccaTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgcc < 3:362243/113‑1 (MQ=255) accaTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAGCGGAGCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAg > 3:1384555/1‑149 (MQ=255) accaTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgcc > 4:362243/1‑114 (MQ=255) aTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc < 3:1108791/87‑1 (MQ=255) aTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc > 4:1108791/1‑87 (MQ=255) aTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgcgc > 3:147455/1‑149 (MQ=255) ttCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAcc < 4:630718/104‑1 (MQ=255) ttCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGt > 2:135108/1‑122 (MQ=255) ttCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGt < 1:135108/122‑1 (MQ=255) ttCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt < 1:664968/132‑1 (MQ=255) ttCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt < 1:462571/132‑1 (MQ=255) ttCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt > 2:462571/1‑132 (MQ=255) ttCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt > 2:664968/1‑132 (MQ=255) ttCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGcc < 1:910971/149‑1 (MQ=255) ttCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGcc > 2:910971/1‑149 (MQ=255) ccGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGt > 2:1278995/1‑149 (MQ=255) tGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGtttt < 4:1384555/149‑1 (MQ=255) gC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTa < 4:147455/149‑1 (MQ=255) c‑cGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTAc < 4:837376/149‑1 (MQ=255) c‑cGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTAc < 1:1278995/149‑1 (MQ=255) | GGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTAC > NC_000913/3256301‑3256591 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |