Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,263,500 G→A 6.2% P54L (CCG→CTG) ‡ tdcD ← propionate kinase/acetate kinase C, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,263,5000GA 6.2% 262.7 / 6.2 80P54L (CCG→CTG) ‡tdcDpropionate kinase/acetate kinase C, anaerobic
Reads supporting (aligned to +/- strand):  ref base G (36/39);  new base A (2/3);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

TTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAAC  >  NC_000913/3263352‑3263645
                                                                                                                                                    |                                                                                                                                                 
ttCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCg                                                                                                                                                   >  1:707487/1‑149 (MQ=255)
     cGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCt                                                                                                                                              <  4:1415638/149‑1 (MQ=255)
     cGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCt                                                                                                                                              <  4:181815/149‑1 (MQ=255)
       gTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTgg                                                                                                                                            <  1:1365965/149‑1 (MQ=255)
       gTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTgg                                                                                                                                            <  1:471991/149‑1 (MQ=255)
         aataatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGct                                                                                                                                          <  3:391826/149‑1 (MQ=255)
                          tAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcc                                                                                                                                      <  3:790946/136‑1 (MQ=255)
                          tAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcc                                                                                                                                      >  4:790946/1‑136 (MQ=255)
                                                 aTGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGAGAGCCAGCACCGCTGGctcac                                                                                                                                       <  2:1341755/112‑3 (MQ=255)
                                                 aTGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGAGAGCCAGCACCGCTGGctcac                                                                                                                                       >  1:1341755/1‑110 (MQ=255)
                                                         gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCt                                                                                                                                              <  4:1073871/97‑1 (MQ=255)
                                                         gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCt                                                                                                                                              >  3:1073871/1‑97 (MQ=255)
                                                         gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                          >  3:445559/1‑149 (MQ=255)
                                                           cAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGtt                                                                                                     <  1:1258085/136‑1 (MQ=255)
                                                           cAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGtt                                                                                                     >  2:1258085/1‑136 (MQ=255)
                                                              ttAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATa                                                                                     >  4:1469866/1‑149 (MQ=255)
                                                                  ggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTg                                                                                 >  4:813995/1‑149 (MQ=255)
                                                                  ggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTg                                                                                 >  2:617060/1‑149 (MQ=255)
                                                                   gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                <  4:445559/149‑1 (MQ=255)
                                                                    ccACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACTGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGAc                                                                               >  1:11550/1‑149 (MQ=255)
                                                                          tGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGt                                                                                                                                                 >  3:690607/1‑77 (MQ=255)
                                                                          tGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGt                                                                                                                                                 <  4:690607/77‑1 (MQ=255)
                                                                          tGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTgg                                                                                                                                            >  3:691700/1‑82 (MQ=255)
                                                                          tGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTgg                                                                                                                                            <  4:691700/82‑1 (MQ=255)
                                                                          tGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt                                                                         <  3:1469866/149‑1 (MQ=255)
                                                                           gTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATa                                                                        <  2:707487/149‑1 (MQ=255)
                                                                              aTTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGACTCTCCCCCATTTACg                                                                                                                            >  4:593891/1‑94 (MQ=255)
                                                                              aTTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGACTCTCCCCCATTTACg                                                                                                                            <  3:593891/94‑1 (MQ=255)
                                                                               tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTgg                                                                                                                                            >  2:599887/1‑77 (MQ=255)
                                                                               tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTgg                                                                                                                                            <  1:599887/77‑1 (MQ=255)
                                                                               tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAt                                                                                                                                 >  2:1323460/1‑88 (MQ=255)
                                                                               tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAt                                                                                                                                 <  1:1323460/88‑1 (MQ=255)
                                                                               tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATaa                                                                                                                       >  4:82776/1‑98 (MQ=255)
                                                                               tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATaa                                                                                                                       <  3:82776/98‑1 (MQ=255)
                                                                               tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAt                                                                                                               <  4:376639/106‑1 (MQ=255)
                                                                               tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAt                                                                                                               >  3:376639/1‑106 (MQ=255)
                                                                               tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTc                                                                    <  2:11550/149‑1 (MQ=255)
                                                                               tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTc                                                                    >  2:995632/1‑149 (MQ=255)
                                                                                ttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCa                                                                                                                <  4:1089269/104‑1 (MQ=255)
                                                                                ttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCa                                                                                                                >  3:1089269/1‑104 (MQ=255)
                                                                                  aaaTTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcaca                                                                 <  3:813995/149‑1 (MQ=255)
                                                                                        cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCt                                                           <  1:617060/149‑1 (MQ=255)
                                                                                         gTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTg                                                          <  1:995632/149‑1 (MQ=255)
                                                                                                cAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                          >  2:345059/1‑110 (MQ=255)
                                                                                                cAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                          <  1:345059/110‑1 (MQ=255)
                                                                                                  gTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGa                                                   <  4:1104935/147‑1 (MQ=255)
                                                                                                  gTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGa                                                   >  3:1104935/1‑147 (MQ=255)
                                                                                                      aaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATa                                                                                                 <  2:893791/97‑1 (MQ=255)
                                                                                                      aaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATa                                                                                                 >  1:893791/1‑97 (MQ=255)
                                                                                                      aaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                >  4:966835/1‑114 (MQ=255)
                                                                                                      aaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                <  3:966835/114‑1 (MQ=255)
                                                                                                       aaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                <  4:697843/113‑1 (MQ=255)
                                                                                                       aaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                >  3:697843/1‑113 (MQ=255)
                                                                                                       aaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCt                                                           >  3:1247400/1‑134 (MQ=255)
                                                                                                       aaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCt                                                           <  4:1247400/134‑1 (MQ=255)
                                                                                                            aaTTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcc                                                                                                                                      <  2:1023507/54‑1 (MQ=255)
                                                                                                            aaTTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcc                                                                                                                                      >  1:1023507/1‑54 (MQ=255)
                                                                                                                   ttCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCg                                                                                                         <  4:183239/76‑1 (MQ=255)
                                                                                                                   ttCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCg                                                                                                         >  3:183239/1‑76 (MQ=255)
                                                                                                                   ttCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGa                                >  1:929950/1‑149 (MQ=255)
                                                                                                                      aaTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGa                             >  1:1074389/1‑149 (MQ=255)
                                                                                                                      aaTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGa                             >  3:1420281/1‑149 (MQ=255)
                                                                                                                       aTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                <  1:1270397/97‑1 (MQ=255)
                                                                                                                       aTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                >  2:1270397/1‑97 (MQ=255)
                                                                                                                          cACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGaa                         <  4:1420281/149‑1 (MQ=255)
                                                                                                                          cACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGaa                         <  2:1074389/149‑1 (MQ=255)
                                                                                                                                   aGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTta                <  2:929950/149‑1 (MQ=255)
                                                                                                                                        tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCAGGGAAAACTTAATCGAAGACGAACcaca                    >  3:974279/1‑140 (MQ=255)
                                                                                                                                        tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACcaca                    <  4:974279/140‑1 (MQ=255)
                                                                                                                                          gtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTc                                                                                                           <  2:1389873/51‑1 (MQ=255)
                                                                                                                                          gtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTc                                                                                                           >  1:1389873/1‑51 (MQ=255)
                                                                                                                                            gAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATa                                                                        >  2:22146/1‑84 (MQ=255)
                                                                                                                                            gAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATa                                                                        <  1:22146/84‑1 (MQ=255)
                                                                                                                                             aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaaaa      >  4:1005621/1‑149 (MQ=255)
                                                                                                                                               ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCg                                                                                           >  2:1319931/1‑62 (MQ=255)
                                                                                                                                               ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCg                                                                                           <  1:1319931/62‑1 (MQ=255)
                                                                                                                                                cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGc                                                                                         <  2:957454/63‑1 (MQ=255)
                                                                                                                                                cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGc                                                                                         >  1:957454/1‑63 (MQ=255)
                                                                                                                                                cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTACACAGTCGCTGGCATCGAGCAc                                               <  4:1463444/105‑1 (MQ=255)
                                                                                                                                                cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTACACAGTCGCTGGCATCGAGCAc                                               >  3:1463444/1‑105 (MQ=255)
                                                                                                                                                 aGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaacaac  >  2:729260/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAAC  >  NC_000913/3263352‑3263645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑