BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,747,173	Δ1 bp	5.3%	coding (90/1497 nt)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,747,173	0	G	.	5.3%	738.8 / 32.7	207	coding (90/1497 nt)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base G (94/102); new base . (5/6); total (99/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

Only 100 of 221 total aligned reads displayed.

TTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCC  >  NC_000913/3747025‑3747320

ttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAg                                                                                                                                                     >  2:20/1‑149 (MQ=255)

 tAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAgc                                                                                                                                                    <  1:149387/149‑1 (MQ=255)

  aaaGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAgcg                                                                                                                                                   >  3:378931/1‑149 (MQ=255)

   aaGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAgcgc                                                                                                                                                  >  2:49688/1‑149 (MQ=255)

     gATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCt                                                                                                                                                <  4:374292/149‑1 (MQ=255)

     gATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCt                                                                                                                                                <  2:523766/149‑1 (MQ=255)

       tgtgCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGc                                                                                                                                              <  3:78816/149‑1 (MQ=255)

                   aGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCAt                                                                                                                                  <  2:8764/149‑1 (MQ=255)

                   aGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCAt                                                                                                                                  <  1:244477/149‑1 (MQ=255)

                   aGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCAt                                                                                                                                  >  3:14024/1‑149 (MQ=255)

                       gATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAg                                                                                                                              <  4:691173/149‑1 (MQ=255)

                        aTCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGc                                                                                                                             >  2:459521/1‑149 (MQ=255)

                        aTCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGc                                                                                                                             >  1:659068/1‑149 (MQ=255)

                          ctgctgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGccc                                                                                                                           <  1:676154/149‑1 (MQ=255)

                              tgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAg                                                                                                                       >  2:221947/1‑149 (MQ=255)

                                aaaGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGcc                                                                                                                     >  4:175965/1‑149 (MQ=255)

                                   gCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAgg                                                                                                                  <  4:89368/149‑1 (MQ=255)

                                    ccGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGc                                                                                                                 <  3:596723/149‑1 (MQ=255)

                                         gATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGc                                                                                                            <  1:5084/149‑1 (MQ=255)

                                               gTGCAATAAACCATGACGCAATCCTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCA‑CGCCTGc                                                                                                                                              <  4:675050/109‑1 (MQ=255)

                                               gTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                                                                                      >  2:187964/1‑149 (MQ=255)

                                                   aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                                                                                  >  2:404566/1‑149 (MQ=255)

                                                   aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                                                                                  >  3:455369/1‑149 (MQ=255)

                                                    aTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                                                                                 <  1:451439/149‑1 (MQ=255)

                                                    aTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                                                                                 >  4:267473/1‑149 (MQ=255)

                                                     tAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                                                                                                <  2:341458/149‑1 (MQ=255)

                                                        aCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGCAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTaa                                                                                                                               <  3:582279/115‑1 (MQ=255)

                                                        aCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGTATTaa                                                                                                                               >  4:582279/1‑115 (MQ=255)

                                                         ccATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCa                                                                                            <  3:673274/149‑1 (MQ=255)

                                                         ccATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCa                                                                                            <  1:221947/149‑1 (MQ=255)

                                                          cATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAg                                                                                           >  4:114176/1‑149 (MQ=255)

                                                                gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                                                                                     <  4:472632/149‑1 (MQ=255)

                                                                gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                                                                                     >  3:256238/1‑149 (MQ=255)

                                                                 cAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGTTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgtg                                                                                    <  2:274876/149‑1 (MQ=255)

                                                                 cAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgtg                                                                                    <  3:266830/149‑1 (MQ=255)

                                                                  aaTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTgg                                                                                   <  1:176277/149‑1 (MQ=255)

                                                                   aTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAAGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGc                                                                                  >  3:611669/1‑149 (MQ=255)

                                                                      ctggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt                                                                               <  1:202494/149‑1 (MQ=255)

                                                                         gctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgct                                                                            >  3:406280/1‑149 (MQ=255)

                                                                         gctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgct                                                                            <  1:7355/149‑1 (MQ=255)

                                                                         gctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgct                                                                            >  1:193315/1‑149 (MQ=255)

                                                                           tggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCa                                                                                                                    >  2:108899/1‑107 (MQ=255)

                                                                           tggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgctgc                                                                          <  1:308220/149‑1 (MQ=255)

                                                                            ggggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa                                                                         <  4:119923/149‑1 (MQ=255)

                                                                               gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg                                                                      >  2:644710/1‑149 (MQ=255)

                                                                               gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg                                                                      >  2:318746/1‑149 (MQ=255)

                                                                                ttAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc                                                                     >  1:549789/1‑149 (MQ=255)

                                                                                 tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCt                                                                    <  1:288661/149‑1 (MQ=255)

                                                                                 tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCt                                                                    <  4:455369/149‑1 (MQ=255)

                                                                                    aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTc                                                                 <  2:549789/149‑1 (MQ=255)

                                                                                    aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTc                                                                 <  3:321774/149‑1 (MQ=255)

                                                                                         ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcg                                                            >  2:134185/1‑149 (MQ=255)

                                                                                               aGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCt                                                      <  4:389539/149‑1 (MQ=255)

                                                                                                  tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGaa                                                                                         >  3:259366/1‑111 (MQ=255)

                                                                                                    gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTa                                                 >  1:542714/1‑149 (MQ=255)

                                                                                                      tgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGc                                                                                                                 >  4:61012/1‑83 (MQ=255)

                                                                                                      tgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACt                                               >  3:313449/1‑149 (MQ=255)

                                                                                                           gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTggg                                                                                                             <  1:104493/82‑1 (MQ=255)

                                                                                                           gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTc                                          <  1:49688/149‑1 (MQ=255)

                                                                                                           gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTc                                          >  1:410213/1‑149 (MQ=255)

                                                                                                           gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTc                                          <  1:357010/149‑1 (MQ=255)

                                                                                                           gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTc                                          <  3:586273/149‑1 (MQ=255)

                                                                                                            ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCt                                         <  4:14024/149‑1 (MQ=255)

                                                                                                            ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCt                                         >  1:479725/1‑149 (MQ=255)

                                                                                                            ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCt                                         <  4:23744/149‑1 (MQ=255)

                                                                                                              gggCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTgg                                       <  3:175965/149‑1 (MQ=255)

                                                                                                              gggCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTgg                                       >  1:634812/1‑149 (MQ=255)

                                                                                                                  tGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgtgt                                    >  3:479046/1‑148 (MQ=255)

                                                                                                                    tATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAg                                 >  3:237745/1‑149 (MQ=255)

                                                                                                                     aTGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGc                                >  1:602161/1‑149 (MQ=255)

                                                                                                                      tGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgt                                      >  4:191499/1‑142 (MQ=255)

                                                                                                                       gACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgg                              <  1:134185/149‑1 (MQ=255)

                                                                                                                       gACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgg                              <  4:261573/149‑1 (MQ=255)

                                                                                                                         ccGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgggg                            >  3:107892/1‑149 (MQ=255)

                                                                                                                           gcgAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGaa                          >  1:402929/1‑149 (MQ=255)

                                                                                                                            cgAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCa                                                                                            <  4:315856/82‑1 (MQ=255)

                                                                                                                                 gCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAAtt                    <  2:479725/149‑1 (MQ=255)

                                                                                                                                 gCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAAtt                    <  2:660493/149‑1 (MQ=255)

                                                                                                                                   cGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCt                                                      <  3:345585/113‑1 (MQ=255)

                                                                                                                                   cGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCt                                                      >  4:345585/1‑113 (MQ=255)

                                                                                                                                   cGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGt                  >  2:629834/1‑149 (MQ=255)

                                                                                                                                    gggAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgggg                            <  3:507280/138‑1 (MQ=255)

                                                                                                                                    gggAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTc                 <  2:541305/149‑1 (MQ=255)

                                                                                                                                    gggAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGACCGCGATATGGCAGAACTGTGGCAATGCTGCATAGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgggg                            >  4:507280/1‑138 (MQ=255)

                                                                                                                                     ggAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCg                >  2:435091/1‑149 (MQ=255)

                                                                                                                                     ggAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCg                <  1:459521/149‑1 (MQ=255)

                                                                                                                                        ggcaggcGCTGCA‑CGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGt                  <  2:209210/144‑1 (MQ=255)

                                                                                                                                        ggcaggcG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTa             >  2:58131/1‑149 (MQ=255)

                                                                                                                                         gcaggcGCTGCA‑CGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTAt            >  3:500161/1‑149 (MQ=255)

                                                                                                                                           aggcG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCg          <  1:187964/149‑1 (MQ=255)

                                                                                                                                           aggcG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCg          >  3:240665/1‑149 (MQ=255)

                                                                                                                                           aggcG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCg          <  2:634812/149‑1 (MQ=255)

                                                                                                                                             gcG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGc        >  2:438099/1‑149 (MQ=255)

                                                                                                                                               g‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCAt      <  4:256238/149‑1 (MQ=255)

                                                                                                                                                 tGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACGGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATGCTGGTGTTAGCTGGGAACAAATTGTCGGTATCGGCAtc     >  4:179402/1‑149 (MQ=255)

                                                                                                                                                  gCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTa             <  3:119136/140‑1 (MQ=255)

                                                                                                                                                    aGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTcc  <  2:602161/149‑1 (MQ=255)

                                                                                                                                                    aGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTcc  >  3:160631/1‑149 (MQ=255)

                                                                                                                                                     gcgcCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgg                              <  1:218021/120‑1 (MQ=255)

                                                                                                                                                     gcgcCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAAt                     <  2:559342/129‑1 (MQ=255)

TTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCC  >  NC_000913/3747025‑3747320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`