Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 813,570:1 +T 6.5% coding (45/2022 nt) uvrB → exision nuclease of nucleotide excision repair, DNA damage recognition component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913813,5701.T 6.5% 253.3 / 13.2 62coding (45/2022 nt)uvrBexision nuclease of nucleotide excision repair, DNA damage recognition component
Reads supporting (aligned to +/- strand):  ref base . (29/29);  new base T (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

TGGTGATGAACTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGC  >  NC_000913/813424‑813718
                                                                                                                                                   |                                                                                                                                                    
tGGTGATGAACTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGa                                                                                                                                                    <  2:60892/149‑1 (MQ=255)
   tgatgaACTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCa                                                                                                                                                 <  1:257374/149‑1 (MQ=255)
       gaACTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCa                                                                                                                                             >  3:396561/1‑149 (MQ=255)
         aCTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCCTCTGGCTGATCGCCaga                                                                                                                                           >  2:51904/1‑149 (MQ=255)
          cTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCCTCTGGCTGATCGCCagaa                                                                                                                                          <  1:51904/149‑2 (MQ=255)
           tGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAgg                                                                                                                                         >  1:53911/1‑149 (MQ=255)
                     tCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACg                                                                                                                               >  3:440627/1‑149 (MQ=255)
                        aGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCTGATCGCCagaagg                                                                                                                                        >  2:55920/1‑134 (MQ=255)
                        aGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCTGATCGCCagaagg                                                                                                                                        <  1:55920/137‑4 (MQ=255)
                        aGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGtct                                                                                                                            <  4:275447/149‑1 (MQ=255)
                              aTTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAaga                                                                                                                      <  3:375008/149‑1 (MQ=255)
                                  gttgGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAgggg                                                                                                                  <  4:440627/149‑1 (MQ=255)
                                      gCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTgg                                                                                                              >  2:439511/1‑149 (MQ=255)
                                           aTTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGAt                                                                                                         >  1:392551/1‑149 (MQ=255)
                                            ttAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATg                                                                                                        >  4:250282/1‑149 (MQ=255)
                                            ttAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATg                                                                                                        <  1:674875/149‑1 (MQ=255)
                                                 tACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAaga                                                                                                                      <  3:498489/130‑1 (MQ=255)
                                                 tACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAaga                                                                                                                      >  4:498489/1‑130 (MQ=255)
                                                 tACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTg                                                                                                   <  4:396561/149‑1 (MQ=255)
                                                          tAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAg                                                                                          >  1:657782/1‑149 (MQ=255)
                                                           aaaaTTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGa                                                                                         <  1:419065/149‑1 (MQ=255)
                                                              aTTACATACCTGCCCGCCCAACCCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGt                                                                                      <  3:342966/149‑1 (MQ=255)
                                                                   aTACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGa                                                                                         <  2:65276/141‑1 (MQ=255)
                                                                   aTACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGa                                                                                         >  1:206399/1‑141 (MQ=255)
                                                                   aTACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGa                                                                                         <  2:206399/141‑1 (MQ=255)
                                                                   aTACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGa                                                                                         >  1:65276/1‑141 (MQ=255)
                                                                   aTACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGATGGAAGATGGCCTGGCGCACCAGACGTTACtt                                                                                 <  2:116336/149‑1 (MQ=255)
                                                                     aCCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGt                                                                                      <  1:147661/142‑1 (MQ=255)
                                                                     aCCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGt                                                                                      >  2:147661/1‑142 (MQ=255)
                                                                      ccTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGc                                                                              <  3:250282/149‑1 (MQ=255)
                                                                      ccTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAAACGTTACTTGGc                                                                              <  3:50200/149‑1 (MQ=255)
                                                                        tGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGt                                                                            <  1:455710/149‑1 (MQ=255)
                                                                                cAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCt                                                                    >  2:579190/1‑149 (MQ=255)
                                                                                 aaCTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTc                                                                   >  2:614294/1‑149 (MQ=255)
                                                                                  aCTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAACCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCa                                                                  <  2:45791/149‑1 (MQ=255)
                                                                                    tCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAgg                                                                <  3:267246/149‑1 (MQ=255)
                                                                                          aGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGAt                                                                                                                                     <  2:424549/74‑1 (MQ=255)
                                                                                          aGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGAt                                                                                                                                     >  1:424549/1‑74 (MQ=255)
                                                                                           ggTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAAcc                                                         >  2:10456/1‑149 (MQ=255)
                                                                                                      aTGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCAtt                                                >  2:680788/1‑147 (MQ=255)
                                                                                                      aTGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCAtt                                                <  1:680788/147‑1 (MQ=255)
                                                                                                             aaCCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTc                                       >  2:297634/1‑149 (MQ=255)
                                                                                                             aaCCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTc                                       >  2:122009/1‑149 (MQ=255)
                                                                                                                cGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCAtt                                    <  1:614294/149‑1 (MQ=255)
                                                                                                                cGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCAtt                                    <  2:53911/149‑1 (MQ=255)
                                                                                                                cGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCAtt                                    <  2:392551/149‑1 (MQ=255)
                                                                                                                 gTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTg                                   >  2:62497/1‑149 (MQ=255)
                                                                                                                      aaCTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGAc                                                                                        <  4:147804/91‑1 (MQ=255)
                                                                                                                      aaCTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGAc                                                                                        >  3:147804/1‑91 (MQ=255)
                                                                                                                       aCTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCAtt                                                >  3:192441/1‑130 (MQ=255)
                                                                                                                       aCTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCAtt                                                <  4:192441/130‑1 (MQ=255)
                                                                                                                            aTTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAg                        <  1:122009/149‑1 (MQ=255)
                                                                                                                             ttCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAgc                       >  4:296121/1‑149 (MQ=255)
                                                                                                                                cGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGcc                    >  3:363927/1‑149 (MQ=255)
                                                                                                                                       aaaCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCAt             >  4:268386/1‑149 (MQ=255)
                                                                                                                                        aaCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATg            >  2:473238/1‑149 (MQ=255)
                                                                                                                                         aCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATgg           >  3:225856/1‑149 (MQ=255)
                                                                                                                                         aCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATgg           >  2:450656/1‑149 (MQ=255)
                                                                                                                                            ttCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCAc                                                    >  3:205731/1‑105 (MQ=255)
                                                                                                                                            ttCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCAc                                                    <  4:205731/105‑1 (MQ=255)
                                                                                                                                            ttCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTAc        >  1:285370/1‑149 (MQ=255)
                                                                                                                                                ggCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTgc    <  1:579190/149‑1 (MQ=255)
                                                                                                                                                  cGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTgcgc  <  1:473238/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGGTGATGAACTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGC  >  NC_000913/813424‑813718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: