Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	4,183,116	(45 bp)1→2	20.4%	coding (1872/4029 nt)	rpoB →	RNA polymerase, beta subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4183072 =	93 (1.230)	21 (0.300)	19/242	NT	20.4%	coding (1828/4029 nt)	rpoB	RNA polymerase, beta subunit
	?	NC_000913	= 4183116	77 (1.090)					coding (1872/4029 nt)	rpoB	RNA polymerase, beta subunit

TACCACCTGCTGGGTGGATACGTCCATGTAGTCAACCTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4183227‑4183072 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccttcttcATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTCAAGGAAGCCGTATTCGTTAGTCTGTGCGTACACGGA  <  NC_000913/4183116‑4182970                                                                                                                                                                                                                                                                                                                 TACCACCTGCTGGGTGGATACGTCCATGTAGTCAACCTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGC                                                                                                                                                            >  1:323355/1‑149    CACCTGCTGGGTGGATACGTCCATGTAGTCAACCTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTT                                                                                                                                                         >  2:643920/1‑149     ACCTGCTGGGTGGATACGTCCATGTAGTCAACCTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTC                                                                                                                                                        >  1:134501/1‑149     ACCTGCTGGGTGGATACGTCCATGTAGTCAACCTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTC                                                                                                                                                        <  3:655264/149‑1       ccagcaGGTGGATACGTCCATGTAGTCAACCTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTT                                                                                                                                                      <  2:521755/143‑1       CTGCTGGGTGGATACGTCCATGTAGTCAACCTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTT                                                                                                                                                      <  4:123355/149‑1        TGCTGGGTGGATACGTCCATGTAGTCAACCTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTC                                                                                                                                                     <  3:530265/149‑1                               GTCAACCTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGG                                                                                                                              >  2:289257/1‑149                                  AACCTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGA                                                                                                                           >  4:383013/1‑149                                     CTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAA                                                                                                                        <  1:289257/149‑1                                           GCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGT                                                                                                                  <  4:633499/149‑1                                                                                     CAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTA                                                                        <  3:383013/149‑1                                                                                      AGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTAC                                                                       >  2:7446/1‑149                                                                                      AGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTAC                                                                       >  1:476998/1‑149                                                                                                  AAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGTGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAA                                                                                    <  2:588163/124‑1                                                                                                  AAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAA                                                                                    >  1:588163/1‑124                                                                                                     TGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCAC                                                        >  2:34339/1‑149                                                                                                         CTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTA                                                    >  1:16340/1‑149                                                                                                          TTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTAC                                                   >  2:89185/1‑149                                                                                                          TTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTAC                                                   <  1:89185/149‑1                                                                                                            CTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGA                                                 <  4:210464/149‑1                                                                                                             TTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGAT                                                >  2:415211/1‑149                                                                                                                 TCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGG                                            >  2:548838/1‑149                                                                                                                     AGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTC                                        <  1:34339/149‑1                                                                                                                       TTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTC                                      >  3:64401/1‑149                                                                                                                                 CCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTAC                                                   <  4:246138/126‑1                                                                                                                                 CCTGGGCGATAACGTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTAC                                                   >  3:246138/1‑126                                                                                                                                              GTAGTTGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTCAAGGAAGCCGTATTCGTTAGTCT               >  3:78848/1‑149                                                                                                                                                    GCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTCAAGGAAGCCGTATTCGTTAGTCTGTGCGT         >  1:220304/1‑149                                                                                                                                                      CTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTATGATACGGAGTCTCAAGGAAGCCGTATTCGTTAGTCTGTGCGTAC       >  2:34337/1‑149                                                                                                                                                      CTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTCAAGGAAGCCGTATTCGTTAGTCTGTGCGTAC       >  4:457151/1‑149                                                                                                                                                      CTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTCAAGGAAGCCGTATTAGTTAGTCTGTGCGTAC       >  1:534215/1‑149                                                                                                                                                       TTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTCAAGGAAGCCGTATTCGTTAGTCTGTGCGTACA      >  3:640290/1‑149                                                                                                                                                       TTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTCAAGGAAGCCGTATTCGTTAGTCTGTGCGTACA      >  1:249444/1‑149                                                                                                                                                       TTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTCAAGGAAGCCGTATTCGTTAGTCTGTGCGTACA      >  3:504991/1‑149                                                                                                                                                         CTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTCAAGGAAGCCGTATTCGTTAGTCTGTGCGTACACG    >  4:9156/1‑149                                                                                                                                                           TCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTCAAGGAAGCCGTATTCGTTAGTCTGTGCGTACACGGA  <  1:136458/149‑1                                                                                                                                                                                                                                                                                                                 TACCACCTGCTGGGTGGATACGTCCATGTAGTCAACCTGGTCGCGGCTGAACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4183227‑4183072 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccttcttcATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTCAGTTACAACACCGTCGGTCACTTTACGATACGGAGTCTCAAGGAAGCCGTATTCGTTAGTCTGTGCGTACACGGA  <  NC_000913/4183116‑4182970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.