Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 747,895 C→G 5.4% intergenic (‑126/+26) abrB ← / ← ybgO regulator of aidB expression; inner membrane protein/putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913747,8950CG 5.4% 263.6 / 4.6 74intergenic (‑126/+26)abrB/ybgOregulator of aidB expression; inner membrane protein/putative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base C (35/35);  new base G (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCAC  >  NC_000913/747752‑748036
                                                                                                                                                |                                                                                                                                             
ccACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑Ccggcg                                                                                                                                          >  3:118940/1‑149 (MQ=255)
    tGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCaa                                                                                                                                      <  2:494067/149‑1 (MQ=255)
    tGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCaa                                                                                                                                      <  3:470616/149‑1 (MQ=255)
     gCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCaaa                                                                                                                                     >  1:687658/1‑149 (MQ=255)
       aaaaCTGGCATCCTTCGCTCTCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAgg                                                                                                                                   <  4:231840/149‑1 (MQ=255)
       aaaaCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAgg                                                                                                                                   >  4:213606/1‑149 (MQ=255)
         aaCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGc                                                                                                                                 >  4:519267/1‑149 (MQ=255)
          aCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑GCCGCCGGAAAGGGca                                                                                                                                <  3:656035/149‑1 (MQ=255)
          aCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGca                                                                                                                                <  3:493349/149‑1 (MQ=255)
           cTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGG‑AAAGGGcat                                                                                                                               <  3:223132/149‑1 (MQ=255)
            tGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGcatc                                                                                                                              >  2:232695/1‑149 (MQ=255)
            tGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGca                                                                                                                                >  2:380132/1‑147 (MQ=255)
            tGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGca                                                                                                                                <  1:380132/147‑1 (MQ=255)
                aTCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑GCCGCCGGAAAGGGc                                                                                                                                 >  1:470841/1‑142 (MQ=255)
                aTCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑GCCGCCGGAAAGGGc                                                                                                                                 <  2:470841/142‑1 (MQ=255)
                 tCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATATAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGt                                                                                                                         >  4:401380/1‑149 (MQ=255)
                 tCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGt                                                                                                                         >  3:167622/1‑149 (MQ=255)
                    ttCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTaaa                                                                                                                      >  3:519018/1‑149 (MQ=255)
                           ccTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTta                                                                                                               <  3:401380/149‑1 (MQ=255)
                           ccTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTta                                                                                                               <  4:167622/149‑1 (MQ=255)
                           ccTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTta                                                                                                               >  4:220735/1‑149 (MQ=255)
                           ccTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTta                                                                                                               >  1:620980/1‑149 (MQ=255)
                                ttattTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGATATAGtt                                                                                                          >  2:306980/1‑149 (MQ=255)
                                     ttCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCt                                                                                                     >  3:396288/1‑149 (MQ=255)
                                         ctTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaa                                                                                                 >  1:493671/1‑149 (MQ=255)
                                          tttGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCaaa                                                                                                                                     <  3:260552/112‑1 (MQ=255)
                                          tttGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCaaa                                                                                                                                     >  4:260552/1‑112 (MQ=255)
                                             ggTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGc                                                                                             <  2:493671/149‑1 (MQ=255)
                                               tgtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGcac                                                                                           <  3:220735/149‑1 (MQ=255)
                                                gtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCATTCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGc                                                                                             <  3:355344/146‑1 (MQ=255)
                                                gtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGc                                                                                             >  4:355344/1‑146 (MQ=255)
                                                 tATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGcacca                                                                                         >  2:13078/1‑149 (MQ=255)
                                                   tCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACt                                                                                       >  4:11765/1‑149 (MQ=255)
                                                         cAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCg                                                                                 >  1:53358/1‑149 (MQ=255)
                                                            cATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAg                                                                              >  4:556329/1‑149 (MQ=255)
                                                             aTAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAgg                                                                             >  2:505377/1‑149 (MQ=255)
                                                             aTAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAgg                                                                             <  3:213606/149‑1 (MQ=255)
                                                              tAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAggg                                                                            >  4:667592/1‑149 (MQ=255)
                                                                            ccAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgg                                                              <  3:519267/149‑1 (MQ=255)
                                                                            ccAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgg                                                              <  3:617466/149‑1 (MQ=255)
                                                                            ccAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgg                                                              <  1:306980/149‑1 (MQ=255)
                                                                             cAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATggg                                                             <  2:550603/149‑1 (MQ=255)
                                                                              aGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgggg                                                            <  2:620980/149‑1 (MQ=255)
                                                                                gAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGt                                                          >  4:140474/1‑149 (MQ=255)
                                                                                  aaaaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGc                                                        <  2:53358/149‑1 (MQ=255)
                                                                                   aaaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGcc                                                       >  1:293918/1‑149 (MQ=255)
                                                                                    aaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCt                                                      >  2:354360/1‑149 (MQ=255)
                                                                                        aaCAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGaa                                                  <  1:505377/149‑1 (MQ=255)
                                                                                        aaCAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGaa                                                  <  4:112221/149‑1 (MQ=255)
                                                                                            gCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGa                                              <  4:118940/149‑1 (MQ=255)
                                                                                              aaTCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACg                                            >  3:211809/1‑149 (MQ=255)
                                                                                                tCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgg                                                              >  3:137238/1‑129 (MQ=255)
                                                                                                tCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgg                                                              <  4:137238/129‑1 (MQ=255)
                                                                                                 cAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGaaa                                         <  2:467750/149‑1 (MQ=255)
                                                                                                    ggAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGaaa                                         <  1:439752/146‑1 (MQ=255)
                                                                                                    ggAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGaaa                                         >  2:439752/1‑146 (MQ=255)
                                                                                                    ggAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGtt                                      >  4:471765/1‑149 (MQ=255)
                                                                                                           gggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGa                                              >  3:397643/1‑134 (MQ=255)
                                                                                                           gggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGa                                              <  4:397643/134‑1 (MQ=255)
                                                                                                           gggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGt                               <  2:687658/149‑1 (MQ=255)
                                                                                                             gAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGtat                             <  1:344274/149‑1 (MQ=255)
                                                                                                                aaTCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAgg                          <  1:232695/149‑1 (MQ=255)
                                                                                                                  tCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGtt                        <  3:471765/149‑1 (MQ=255)
                                                                                                                       aaTTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTc                   <  3:556329/149‑1 (MQ=255)
                                                                                                                            tCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGa              <  3:140474/149‑1 (MQ=255)
                                                                                                                              tGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCg                  <  2:446645/143‑1 (MQ=255)
                                                                                                                              tGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCg                  >  1:446645/1‑143 (MQ=255)
                                                                                                                               gAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCa           >  1:503411/1‑149 (MQ=255)
                                                                                                                                aaaGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCaa          >  3:18381/1‑149 (MQ=255)
                                                                                                                                      aTGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGaaa                                         >  4:217746/1‑112 (MQ=255)
                                                                                                                                      aTGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGaaa                                         <  3:217746/112‑1 (MQ=255)
                                                                                                                                        gCCCTTT‑GCCGCCGGAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCAc  >  3:202006/1‑149 (MQ=255)
                                                                                                                                         cccTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgggg                                                            <  4:129520/90‑1 (MQ=255)
                                                                                                                                         cccTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgggg                                                            >  3:129520/1‑90 (MQ=255)
                                                                                                                                                |                                                                                                                                             
CCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCAC  >  NC_000913/747752‑748036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑