Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 747,902 C→G 5.6% intergenic (‑133/+19) abrB ← / ← ybgO regulator of aidB expression; inner membrane protein/putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913747,9020CG 5.6% 251.0 / 4.7 73intergenic (‑133/+19)abrB/ybgOregulator of aidB expression; inner membrane protein/putative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base C (34/34);  new base G (2/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGGC  >  NC_000913/747756‑748049
                                                                                                                                                   |                                                                                                                                                   
tGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCaa                                                                                                                                                   <  2:494067/149‑1 (MQ=255)
tGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCaa                                                                                                                                                   <  3:470616/149‑1 (MQ=255)
 gCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCaaa                                                                                                                                                  >  1:687658/1‑149 (MQ=255)
   aaaaCTGGCATCCTTCGCTCTCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAgg                                                                                                                                                <  4:231840/149‑1 (MQ=255)
   aaaaCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAgg                                                                                                                                                >  4:213606/1‑149 (MQ=255)
     aaCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGc                                                                                                                                              >  4:519267/1‑149 (MQ=255)
      aCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑GCCGCCGGAAAGGGca                                                                                                                                             <  3:656035/149‑1 (MQ=255)
      aCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGca                                                                                                                                             <  3:493349/149‑1 (MQ=255)
       cTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGG‑AAAGGGcat                                                                                                                                            <  3:223132/149‑1 (MQ=255)
        tGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGcatc                                                                                                                                           >  2:232695/1‑149 (MQ=255)
        tGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGca                                                                                                                                             >  2:380132/1‑147 (MQ=255)
        tGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGca                                                                                                                                             <  1:380132/147‑1 (MQ=255)
            aTCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑GCCGCCGGAAAGGGc                                                                                                                                              >  1:470841/1‑142 (MQ=255)
            aTCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑GCCGCCGGAAAGGGc                                                                                                                                              <  2:470841/142‑1 (MQ=255)
             tCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATATAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGt                                                                                                                                      >  4:401380/1‑149 (MQ=255)
             tCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGt                                                                                                                                      >  3:167622/1‑149 (MQ=255)
                ttCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTaaa                                                                                                                                   >  3:519018/1‑149 (MQ=255)
                       ccTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTta                                                                                                                            >  1:620980/1‑149 (MQ=255)
                       ccTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTta                                                                                                                            >  4:220735/1‑149 (MQ=255)
                       ccTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTta                                                                                                                            <  3:401380/149‑1 (MQ=255)
                       ccTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTta                                                                                                                            <  4:167622/149‑1 (MQ=255)
                            ttattTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGATATAGtt                                                                                                                       >  2:306980/1‑149 (MQ=255)
                                 ttCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCt                                                                                                                  >  3:396288/1‑149 (MQ=255)
                                     ctTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaa                                                                                                              >  1:493671/1‑149 (MQ=255)
                                      tttGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCaaa                                                                                                                                                  <  3:260552/112‑1 (MQ=255)
                                      tttGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCaaa                                                                                                                                                  >  4:260552/1‑112 (MQ=255)
                                         ggTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGc                                                                                                          <  2:493671/149‑1 (MQ=255)
                                           tgtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGcac                                                                                                        <  3:220735/149‑1 (MQ=255)
                                            gtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCATTCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGc                                                                                                          <  3:355344/146‑1 (MQ=255)
                                            gtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGc                                                                                                          >  4:355344/1‑146 (MQ=255)
                                             tATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGcacca                                                                                                      >  2:13078/1‑149 (MQ=255)
                                               tCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACt                                                                                                    >  4:11765/1‑149 (MQ=255)
                                                     cAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCg                                                                                              >  1:53358/1‑149 (MQ=255)
                                                        cATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAg                                                                                           >  4:556329/1‑149 (MQ=255)
                                                         aTAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAgg                                                                                          >  2:505377/1‑149 (MQ=255)
                                                         aTAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAgg                                                                                          <  3:213606/149‑1 (MQ=255)
                                                          tAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAggg                                                                                         >  4:667592/1‑149 (MQ=255)
                                                                        ccAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgg                                                                           <  3:617466/149‑1 (MQ=255)
                                                                        ccAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgg                                                                           <  1:306980/149‑1 (MQ=255)
                                                                        ccAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgg                                                                           <  3:519267/149‑1 (MQ=255)
                                                                         cAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATggg                                                                          <  2:550603/149‑1 (MQ=255)
                                                                          aGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgggg                                                                         <  2:620980/149‑1 (MQ=255)
                                                                            gAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGt                                                                       >  4:140474/1‑149 (MQ=255)
                                                                              aaaaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGc                                                                     <  2:53358/149‑1 (MQ=255)
                                                                               aaaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGcc                                                                    >  1:293918/1‑149 (MQ=255)
                                                                                aaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCt                                                                   >  2:354360/1‑149 (MQ=255)
                                                                                    aaCAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGaa                                                               <  4:112221/149‑1 (MQ=255)
                                                                                    aaCAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGaa                                                               <  1:505377/149‑1 (MQ=255)
                                                                                        gCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGa                                                           <  4:118940/149‑1 (MQ=255)
                                                                                          aaTCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACg                                                         >  3:211809/1‑149 (MQ=255)
                                                                                            tCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgg                                                                           <  4:137238/129‑1 (MQ=255)
                                                                                            tCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgg                                                                           >  3:137238/1‑129 (MQ=255)
                                                                                             cAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGaaa                                                      <  2:467750/149‑1 (MQ=255)
                                                                                                ggAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGaaa                                                      <  1:439752/146‑1 (MQ=255)
                                                                                                ggAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGaaa                                                      >  2:439752/1‑146 (MQ=255)
                                                                                                ggAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGtt                                                   >  4:471765/1‑149 (MQ=255)
                                                                                                       gggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGa                                                           >  3:397643/1‑134 (MQ=255)
                                                                                                       gggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGa                                                           <  4:397643/134‑1 (MQ=255)
                                                                                                       gggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGt                                            <  2:687658/149‑1 (MQ=255)
                                                                                                         gAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGtat                                          <  1:344274/149‑1 (MQ=255)
                                                                                                            aaTCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAgg                                       <  1:232695/149‑1 (MQ=255)
                                                                                                              tCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGtt                                     <  3:471765/149‑1 (MQ=255)
                                                                                                                   aaTTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTc                                <  3:556329/149‑1 (MQ=255)
                                                                                                                        tCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGa                           <  3:140474/149‑1 (MQ=255)
                                                                                                                          tGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCg                               <  2:446645/143‑1 (MQ=255)
                                                                                                                          tGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCg                               >  1:446645/1‑143 (MQ=255)
                                                                                                                           gAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCa                        >  1:503411/1‑149 (MQ=255)
                                                                                                                            aaaGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCaa                       >  3:18381/1‑149 (MQ=255)
                                                                                                                                  aTGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGaaa                                                      <  3:217746/112‑1 (MQ=255)
                                                                                                                                  aTGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGaaa                                                      >  4:217746/1‑112 (MQ=255)
                                                                                                                                    gCCCTTT‑GCCGCCGGAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCAc               >  3:202006/1‑149 (MQ=255)
                                                                                                                                     cccTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgggg                                                                         >  3:129520/1‑90 (MQ=255)
                                                                                                                                     cccTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgggg                                                                         <  4:129520/90‑1 (MQ=255)
                                                                                                                                                  gcAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGGc  <  1:13078/149‑1 (MQ=255)
                                                                                                                                                  gcAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGGc  <  3:667592/149‑1 (MQ=255)
                                                                                                                                                  gcAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGGc  <  1:354360/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTT‑CCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGGC  >  NC_000913/747756‑748049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑