Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 848,356 T→A 9.4% intergenic (‑352/+52) glnH ← / ← dps glutamine transporter subunit/Fe‑binding and storage protein; stress‑inducible DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913848,3560TA 9.4% 318.9 / 19.9 96intergenic (‑352/+52)glnH/dpsglutamine transporter subunit/Fe‑binding and storage protein; stress‑inducible DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (42/45);  new base A (5/4);  total (47/49)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

TTAGCGTAAAACCTGATGAAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCC  >  NC_000913/848208‑848503
                                                                                                                                                    |                                                                                                                                                   
ttAGCGTAAAACCTGATGAAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGt                                                                                                                                                     <  1:678252/149‑1 (MQ=255)
            cTGATGAAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCt                                                                                                                                         <  2:434125/149‑1 (MQ=255)
              gatgaAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCttt                                                                                                                                       <  3:633967/149‑1 (MQ=255)
               atgaAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCtttt                                                                                                                                      >  4:175940/1‑149 (MQ=255)
                 gaAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTAt                                                                                                                                    >  2:666332/1‑149 (MQ=255)
                  aaaaaaCAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATc                                                                                                                                   >  3:391314/1‑149 (MQ=255)
                      aaCAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGt                                                                                                                               >  1:186190/1‑149 (MQ=255)
                                 tGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCt                                                                                                                    >  3:210684/1‑149 (MQ=255)
                                   tAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGc                                                                                                                  >  3:687923/1‑149 (MQ=255)
                                    aaCGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTa                                                                                                                              >  1:211206/1‑136 (MQ=255)
                                    aaCGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTa                                                                                                                              <  2:211206/136‑1 (MQ=255)
                                       gATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCa                                                                                                              >  3:37272/1‑149 (MQ=255)
                                           gtgtgATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAg                                                                                                          <  4:97767/149‑1 (MQ=255)
                                              tgatgaTGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGa                                                                                                       <  4:123017/149‑1 (MQ=255)
                                              tgatgaTGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGa                                                                                                       >  1:355338/1‑149 (MQ=255)
                                               gatgatGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCtt                                                                                                                                        <  1:481047/115‑1 (MQ=255)
                                               gatgatGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCtt                                                                                                                                        >  2:481047/1‑115 (MQ=255)
                                               gatgatGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGAt                                                                                                      <  2:611390/149‑1 (MQ=255)
                                                atgatgTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATg                                                                                                     <  2:627196/149‑1 (MQ=255)
                                                  gatgTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTc                                                                                                                     <  3:208895/131‑1 (MQ=255)
                                                  gatgTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTc                                                                                                                     >  4:208895/1‑131 (MQ=255)
                                                    tgtgGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGAtt                                                                                                 <  4:566343/149‑1 (MQ=255)
                                                       ggATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTAt                                                                                              >  2:341663/1‑149 (MQ=255)
                                                        gATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTAtt                                                                                             >  4:508389/1‑149 (MQ=255)
                                                        gATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTAtt                                                                                             >  2:464794/1‑149 (MQ=255)
                                                         aTACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTc                                                                                            >  3:255683/1‑149 (MQ=255)
                                                          tACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCg                                                                                           <  4:210684/149‑1 (MQ=255)
                                                            cAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATCAGTGCGTTGAGGAGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGAt                                                                                         >  4:507906/1‑149 (MQ=255)
                                                            cAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGAt                                                                                         <  3:543311/149‑1 (MQ=255)
                                                             aaaaaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATg                                                                                        >  4:423217/1‑149 (MQ=255)
                                                              aaaaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGt                                                                                       >  1:457006/1‑149 (MQ=255)
                                                              aaaaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGt                                                                                       >  1:565973/1‑149 (MQ=255)
                                                                aaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCTTCCTGACAGGAGGCCGttt                                                                                                                                        >  3:524209/1‑98 (MQ=38)
                                                                aaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCTTCCTGACAGGAGGCCGttt                                                                                                                                        <  4:524209/98‑1 (MQ=39)
                                                                aaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTa                                                                                     >  4:260416/1‑149 (MQ=255)
                                                                  aTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGa                                                                                   <  4:37272/149‑1 (MQ=255)
                                                                  aTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGa                                                                                   <  3:175940/149‑1 (MQ=255)
                                                                     tACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTc                                                                                <  4:564731/149‑1 (MQ=255)
                                                                       cTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGa                                                                              <  2:520339/149‑1 (MQ=255)
                                                                        ttAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGAt                                                                             >  3:171015/1‑149 (MQ=255)
                                                                           aTCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATaaa                                                                          >  1:499033/1‑149 (MQ=255)
                                                                            tCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAAc                                                                         <  1:158461/149‑1 (MQ=255)
                                                                            tCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCAGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAAc                                                                         >  3:645091/1‑149 (MQ=255)
                                                                                cTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACcaca                                                                     <  4:255683/149‑1 (MQ=255)
                                                                                    agaTAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGaa                                                                 <  1:359375/149‑1 (MQ=255)
                                                                                     gaTAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAAt                                                                <  3:508389/149‑1 (MQ=255)
                                                                                     gaTAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAAt                                                                <  1:464794/149‑1 (MQ=255)
                                                                                         gCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGaa                                                                 >  3:187781/1‑144 (MQ=255)
                                                                                         gCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGaa                                                                 <  4:187781/144‑1 (MQ=255)
                                                                                          cAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATc                                                           <  2:355338/149‑1 (MQ=255)
                                                                                           aGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGAt                                                                                                      >  4:64922/1‑105 (MQ=255)
                                                                                           aGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGAt                                                                                                      <  3:64922/105‑1 (MQ=255)
                                                                                           aGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATcc                                                          <  4:534623/149‑1 (MQ=255)
                                                                                               ggatgCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGt                                                      >  3:600011/1‑149 (MQ=255)
                                                                                                gatgCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTc                                                     <  4:391314/149‑1 (MQ=255)
                                                                                                  tgCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTcgc                                                   <  1:341663/149‑1 (MQ=255)
                                                                                                    cACTAAATAAGTGCGTTGAGGTTGGCTGCAAAACAAAACAGCCACCTGTCAGGAAGCCGCTTTTATCGGGTACTAAACTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAAATTATCCAGGTCGCga                                                 <  4:645091/149‑1 (MQ=255)
                                                                                                     aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTAt                                                                                              >  2:348514/1‑103 (MQ=255)
                                                                                                     aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTAt                                                                                              <  1:348514/103‑1 (MQ=255)
                                                                                                     aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACcaca                                                                     <  3:350968/128‑1 (MQ=255)
                                                                                                     aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACcaca                                                                     >  4:350968/1‑128 (MQ=255)
                                                                                                     aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTAt                                                            <  1:682042/137‑1 (MQ=255)
                                                                                                     aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTAt                                                            >  2:682042/1‑137 (MQ=255)
                                                                                                     aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCgag                                                >  1:503419/1‑149 (MQ=255)
                                                                                                         aaTAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGAcgc                                            >  4:226617/1‑149 (MQ=255)
                                                                                                             aGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACgcggcg                                        <  1:666332/149‑1 (MQ=255)
                                                                                                              gTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGcggcgg                                       >  1:139881/1‑149 (MQ=255)
                                                                                                               tGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGa                                                                                                   <  2:57883/88‑1 (MQ=255)
                                                                                                               tGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGa                                                                                                   >  1:57883/1‑88 (MQ=255)
                                                                                                                  gTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATcc                                                          >  4:271292/1‑126 (MQ=255)
                                                                                                                  gTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATcc                                                          <  3:271292/126‑1 (MQ=255)
                                                                                                                  gTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAg                                   >  4:542039/1‑149 (MQ=255)
                                                                                                                  gTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAACGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAg                                   <  2:457006/149‑1 (MQ=255)
                                                                                                                              tGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAAc                                                                         >  1:252111/1‑99 (MQ=255)
                                                                                                                              tGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAAc                                                                         <  2:252111/99‑1 (MQ=255)
                                                                                                                              tGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGgt                       >  1:9514/1‑149 (MQ=255)
                                                                                                                                 aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACt                                                                                 >  3:398556/1‑88 (MQ=255)
                                                                                                                                 aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTgtc                    <  3:584160/149‑1 (MQ=255)
                                                                                                                                 aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTgtc                    <  3:260416/149‑1 (MQ=255)
                                                                                                                                 aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTgtc                    <  2:186190/149‑1 (MQ=255)
                                                                                                                                 aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTgtc                    <  1:34239/149‑1 (MQ=255)
                                                                                                                                 aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTGCTGCACCATCAGCGATGGATTTATTCGATGTTAGACt                                                                                 <  4:398556/88‑1 (MQ=255)
                                                                                                                                    acaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTgtcgtc                 >  3:661287/1‑149 (MQ=255)
                                                                                                                                       aagcGGCTTCCTGACAGGAGGCCGTTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATc              >  3:630983/4‑149 (MQ=255)
                                                                                                                                         gcGGCTTCCTGACAGGAGGCCGTTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCtt            >  2:36756/2‑149 (MQ=255)
                                                                                                                                         gcGGCTTCCTGACAGGAGGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACcaca                                                                     >  1:590130/2‑92 (MQ=255)
                                                                                                                                         gcGGCTTCCTGACAGGAGGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACcaca                                                                     <  2:590130/91‑1 (MQ=255)
                                                                                                                                          cGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCttt           >  1:185970/1‑149 (MQ=255)
                                                                                                                                           ggCTTCCTGACAGGAGGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATc                                                           <  4:50752/100‑1 (MQ=255)
                                                                                                                                           ggCTTCCTGACAGGAGGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATc                                                           >  3:50752/1‑100 (MQ=255)
                                                                                                                                              ttcctGACAGGAGGCCGTTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTttcgct       <  1:36756/148‑1 (MQ=255)
                                                                                                                                               tcctGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCt             <  4:153037/142‑1 (MQ=255)
                                                                                                                                               tcctGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCt             >  3:153037/1‑142 (MQ=255)
                                                                                                                                                cctGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTttcgcttc     <  2:185970/149‑1 (MQ=255)
                                                                                                                                                cctGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTttcgcttc     <  3:507906/149‑1 (MQ=255)
                                                                                                                                                cctGTCAGAAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTttcgcttc     <  2:565973/149‑1 (MQ=255)
                                                                                                                                                   gTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGcc  >  4:247832/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTAGCGTAAAACCTGATGAAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCC  >  NC_000913/848208‑848503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: