Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 848,362 A→G 9.3% intergenic (‑358/+46) glnH ← / ← dps glutamine transporter subunit/Fe‑binding and storage protein; stress‑inducible DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913848,3620AG 9.3% 300.7 / 16.3 97intergenic (‑358/+46)glnH/dpsglutamine transporter subunit/Fe‑binding and storage protein; stress‑inducible DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (42/46);  new base G (5/4);  total (47/50)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CTGATGAAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCT  >  NC_000913/848220‑848510
                                                                                                                                              |                                                                                                                                                    
cTGATGAAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCt                                                                                                                                                <  2:434125/149‑1 (MQ=255)
  gatgaAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCttt                                                                                                                                              <  3:633967/149‑1 (MQ=255)
   atgaAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCtttt                                                                                                                                             >  4:175940/1‑149 (MQ=255)
     gaAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTAt                                                                                                                                           >  2:666332/1‑149 (MQ=255)
      aaaaaaCAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATc                                                                                                                                          >  3:391314/1‑149 (MQ=255)
          aaCAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGt                                                                                                                                      >  1:186190/1‑149 (MQ=255)
                     tGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCt                                                                                                                           >  3:210684/1‑149 (MQ=255)
                       tAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGc                                                                                                                         >  3:687923/1‑149 (MQ=255)
                        aaCGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTa                                                                                                                                     <  2:211206/136‑1 (MQ=255)
                        aaCGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTa                                                                                                                                     >  1:211206/1‑136 (MQ=255)
                           gATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCa                                                                                                                     >  3:37272/1‑149 (MQ=255)
                               gtgtgATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAg                                                                                                                 <  4:97767/149‑1 (MQ=255)
                                  tgatgaTGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGa                                                                                                              >  1:355338/1‑149 (MQ=255)
                                  tgatgaTGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGa                                                                                                              <  4:123017/149‑1 (MQ=255)
                                   gatgatGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCtt                                                                                                                                               <  1:481047/115‑1 (MQ=255)
                                   gatgatGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCtt                                                                                                                                               >  2:481047/1‑115 (MQ=255)
                                   gatgatGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGAt                                                                                                             <  2:611390/149‑1 (MQ=255)
                                    atgatgTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATg                                                                                                            <  2:627196/149‑1 (MQ=255)
                                      gatgTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTc                                                                                                                            >  4:208895/1‑131 (MQ=255)
                                      gatgTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTc                                                                                                                            <  3:208895/131‑1 (MQ=255)
                                        tgtgGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGAtt                                                                                                        <  4:566343/149‑1 (MQ=255)
                                           ggATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTAt                                                                                                     >  2:341663/1‑149 (MQ=255)
                                            gATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTAtt                                                                                                    >  4:508389/1‑149 (MQ=255)
                                            gATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTAtt                                                                                                    >  2:464794/1‑149 (MQ=255)
                                             aTACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTc                                                                                                   >  3:255683/1‑149 (MQ=255)
                                              tACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCg                                                                                                  <  4:210684/149‑1 (MQ=255)
                                                cAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATCAGTGCGTTGAGGAGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGAt                                                                                                >  4:507906/1‑149 (MQ=255)
                                                cAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGAt                                                                                                <  3:543311/149‑1 (MQ=255)
                                                 aaaaaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATg                                                                                               >  4:423217/1‑149 (MQ=255)
                                                  aaaaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGt                                                                                              >  1:457006/1‑149 (MQ=255)
                                                  aaaaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGt                                                                                              >  1:565973/1‑149 (MQ=255)
                                                    aaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCTTCCTGACAGGAGGCCGttt                                                                                                                                               >  3:524209/1‑98 (MQ=38)
                                                    aaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCTTCCTGACAGGAGGCCGttt                                                                                                                                               <  4:524209/98‑1 (MQ=39)
                                                    aaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTa                                                                                            >  4:260416/1‑149 (MQ=255)
                                                      aTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGa                                                                                          <  3:175940/149‑1 (MQ=255)
                                                      aTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGa                                                                                          <  4:37272/149‑1 (MQ=255)
                                                         tACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTc                                                                                       <  4:564731/149‑1 (MQ=255)
                                                           cTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGa                                                                                     <  2:520339/149‑1 (MQ=255)
                                                            ttAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGAt                                                                                    >  3:171015/1‑149 (MQ=255)
                                                               aTCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATaaa                                                                                 >  1:499033/1‑149 (MQ=255)
                                                                tCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAAc                                                                                <  1:158461/149‑1 (MQ=255)
                                                                tCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCAGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAAc                                                                                >  3:645091/1‑149 (MQ=255)
                                                                    cTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACcaca                                                                            <  4:255683/149‑1 (MQ=255)
                                                                        agaTAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGaa                                                                        <  1:359375/149‑1 (MQ=255)
                                                                         gaTAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAAt                                                                       <  1:464794/149‑1 (MQ=255)
                                                                         gaTAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAAt                                                                       <  3:508389/149‑1 (MQ=255)
                                                                             gCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGaa                                                                        >  3:187781/1‑144 (MQ=255)
                                                                             gCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGaa                                                                        <  4:187781/144‑1 (MQ=255)
                                                                              cAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATc                                                                  <  2:355338/149‑1 (MQ=255)
                                                                               aGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGAt                                                                                                             <  3:64922/105‑1 (MQ=255)
                                                                               aGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGAt                                                                                                             >  4:64922/1‑105 (MQ=255)
                                                                               aGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATcc                                                                 <  4:534623/149‑1 (MQ=255)
                                                                                   ggatgCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGt                                                             >  3:600011/1‑149 (MQ=255)
                                                                                    gatgCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTc                                                            <  4:391314/149‑1 (MQ=255)
                                                                                      tgCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTcgc                                                          <  1:341663/149‑1 (MQ=255)
                                                                                        cACTAAATAAGTGCGTTGAGGTTGGCTGCAAAACAAAACAGCCACCTGTCAGGAAGCCGCTTTTATCGGGTACTAAACTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAAATTATCCAGGTCGCga                                                        <  4:645091/149‑1 (MQ=255)
                                                                                         aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTAt                                                                                                     <  1:348514/103‑1 (MQ=255)
                                                                                         aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTAt                                                                                                     >  2:348514/1‑103 (MQ=255)
                                                                                         aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACcaca                                                                            <  3:350968/128‑1 (MQ=255)
                                                                                         aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACcaca                                                                            >  4:350968/1‑128 (MQ=255)
                                                                                         aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTAt                                                                   >  2:682042/1‑137 (MQ=255)
                                                                                         aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTAt                                                                   <  1:682042/137‑1 (MQ=255)
                                                                                         aCTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCgag                                                       >  1:503419/1‑149 (MQ=255)
                                                                                             aaTAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGAcgc                                                   >  4:226617/1‑149 (MQ=255)
                                                                                                 aGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACgcggcg                                               <  1:666332/149‑1 (MQ=255)
                                                                                                  gTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGcggcgg                                              >  1:139881/1‑149 (MQ=255)
                                                                                                   tGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGa                                                                                                          <  2:57883/88‑1 (MQ=255)
                                                                                                   tGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGa                                                                                                          >  1:57883/1‑88 (MQ=255)
                                                                                                      gTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATcc                                                                 >  4:271292/1‑126 (MQ=255)
                                                                                                      gTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATcc                                                                 <  3:271292/126‑1 (MQ=255)
                                                                                                      gTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAg                                          >  4:542039/1‑149 (MQ=255)
                                                                                                      gTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAACGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAg                                          <  2:457006/149‑1 (MQ=255)
                                                                                                                  tGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAAc                                                                                <  2:252111/99‑1 (MQ=255)
                                                                                                                  tGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAAc                                                                                >  1:252111/1‑99 (MQ=255)
                                                                                                                  tGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGgt                              >  1:9514/1‑149 (MQ=255)
                                                                                                                     aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACt                                                                                        >  3:398556/1‑88 (MQ=255)
                                                                                                                     aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTgtc                           <  1:34239/149‑1 (MQ=255)
                                                                                                                     aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTgtc                           <  3:260416/149‑1 (MQ=255)
                                                                                                                     aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTgtc                           <  3:584160/149‑1 (MQ=255)
                                                                                                                     aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTgtc                           <  2:186190/149‑1 (MQ=255)
                                                                                                                     aaaacaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTGCTGCACCATCAGCGATGGATTTATTCGATGTTAGACt                                                                                        <  4:398556/88‑1 (MQ=255)
                                                                                                                        acaaaacGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTgtcgtc                        >  3:661287/1‑149 (MQ=255)
                                                                                                                           aagcGGCTTCCTGACAGGAGGCCGTTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATc                     >  3:630983/4‑149 (MQ=255)
                                                                                                                             gcGGCTTCCTGACAGGAGGCCGTTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCtt                   >  2:36756/2‑149 (MQ=255)
                                                                                                                             gcGGCTTCCTGACAGGAGGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACcaca                                                                            >  1:590130/2‑92 (MQ=255)
                                                                                                                             gcGGCTTCCTGACAGGAGGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACcaca                                                                            <  2:590130/91‑1 (MQ=255)
                                                                                                                              cGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCttt                  >  1:185970/1‑149 (MQ=255)
                                                                                                                               ggCTTCCTGACAGGAGGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATc                                                                  <  4:50752/100‑1 (MQ=255)
                                                                                                                               ggCTTCCTGACAGGAGGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATc                                                                  >  3:50752/1‑100 (MQ=255)
                                                                                                                                  ttcctGACAGGAGGCCGTTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTttcgct              <  1:36756/148‑1 (MQ=255)
                                                                                                                                   tcctGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCt                    <  4:153037/142‑1 (MQ=255)
                                                                                                                                   tcctGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCt                    >  3:153037/1‑142 (MQ=255)
                                                                                                                                    cctGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTttcgcttc            <  3:507906/149‑1 (MQ=255)
                                                                                                                                    cctGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTttcgcttc            <  2:185970/149‑1 (MQ=255)
                                                                                                                                    cctGTCAGAAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTttcgcttc            <  2:565973/149‑1 (MQ=255)
                                                                                                                                       gTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGcc         >  4:247832/1‑149 (MQ=255)
                                                                                                                                           ggAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATc     <  4:600011/149‑1 (MQ=255)
                                                                                                                                             aaGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGc   >  4:437879/1‑149 (MQ=255)
                                                                                                                                              aGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCt  <  4:171015/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CTGATGAAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCT  >  NC_000913/848220‑848510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: