Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 887,392 T→A 5.4% intergenic (‑53/‑31) ybjJ ← / → rcdA putative drug efflux MFS transporter, inner membrane protein/transcriptional regulator of csgD and ybiJI; autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913887,3920TA 5.4% 331.8 / 6.6 93intergenic (‑53/‑31)ybjJ/rcdAputative drug efflux MFS transporter, inner membrane protein/transcriptional regulator of csgD and ybiJI; autoregulator
Reads supporting (aligned to +/- strand):  ref base T (46/42);  new base A (3/2);  total (49/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

CCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGG  >  NC_000913/887247‑887539
                                                                                                                                                 |                                                                                                                                                   
ccAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTg                                                                                                                                                  >  4:190020/1‑149 (MQ=255)
ccAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTg                                                                                                                                                  >  1:87523/1‑149 (MQ=255)
 cAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGt                                                                                                                                                 <  3:209804/149‑1 (MQ=255)
  aGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTa                                                                                                                                                >  4:293884/1‑149 (MQ=255)
       gCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACAAATGTACACaa                                                                                                                                           <  2:352862/149‑1 (MQ=255)
            tAACAGCCCTGGCAAAAAGAAGAACATATACACCGCCCAGGTTCGGCGTTTCAATGCATTACGTGGAGAATTTACGGTCATAGATCAAGGCAAAAGAAGAAGAGGGACGACAACATTAGAAGGTTGTGTACATTTGTAAACAATTGCag                                                                                                                                     >  4:144089/1‑149 (MQ=255)
              aCAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCaga                                                                                                                                    >  3:508822/1‑149 (MQ=255)
              aCAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCaga                                                                                                                                    >  4:221863/1‑149 (MQ=255)
                  gCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagagaga                                                                                                                                >  4:551569/1‑149 (MQ=255)
                   ccTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagagagag                                                                                                                               <  3:608391/149‑1 (MQ=255)
                   ccTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagagagag                                                                                                                               <  1:94585/149‑1 (MQ=255)
                    cTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAgg                                                                                                                              <  4:508822/149‑1 (MQ=255)
                     tGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGa                                                                                                                             <  4:617685/149‑1 (MQ=255)
                       gCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGaaa                                                                                                                           <  3:221863/149‑1 (MQ=255)
                       gCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGaaa                                                                                                                           <  3:551569/149‑1 (MQ=255)
                       gCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGaaa                                                                                                                           >  2:314270/1‑149 (MQ=255)
                        cAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAAt                                                                                                                          <  3:619066/149‑1 (MQ=255)
                         aaaaaGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATg                                                                                                                         >  2:534744/1‑149 (MQ=255)
                            aagaagAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACa                                                                                                                      >  3:283281/1‑149 (MQ=255)
                                  aaCATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTAAGGTCATAGATCAGGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTc                                                                                                                >  2:232690/1‑149 (MQ=255)
                                   aCATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAAt                                                                                                                          >  4:352736/1‑138 (MQ=255)
                                   aCATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAAt                                                                                                                          <  3:352736/138‑1 (MQ=255)
                                   aCATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTcg                                                                                                               >  2:186367/1‑149 (MQ=255)
                                     aTAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTcgcg                                                                                                             >  4:32707/1‑149 (MQ=255)
                                     aTAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTcgcg                                                                                                             >  4:417005/1‑149 (MQ=255)
                                      tAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGCTCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAAt                                                                                                                          <  2:224643/135‑1 (MQ=255)
                                      tAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAAt                                                                                                                          >  1:224643/1‑135 (MQ=255)
                                         aCAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagaga                                                                                                                                  >  3:577894/1‑124 (MQ=255)
                                         aCAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagaga                                                                                                                                  <  4:577894/124‑1 (MQ=255)
                                         aCAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTaa                                                                                                         <  3:189782/149‑1 (MQ=255)
                                               cccAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATcc                                                                                                   <  3:56442/149‑1 (MQ=255)
                                                   ggTTCGGCGTTTCAATGCATTACGTGAATAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGTGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCaa                                                                                               <  3:466152/149‑1 (MQ=255)
                                                    gTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAAc                                                                                              >  2:230991/1‑149 (MQ=255)
                                                     ttCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACg                                                                                             <  4:113734/149‑1 (MQ=255)
                                                         gCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCga                                                                                         <  3:190020/149‑1 (MQ=255)
                                                                     aTTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATc                                                                             >  3:391333/1‑149 (MQ=255)
                                                                     aTTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATc                                                                             >  1:490691/1‑149 (MQ=255)
                                                                      ttACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATcc                                                                            >  4:478016/1‑149 (MQ=255)
                                                                          gTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGc                                                                                                                   >  3:666636/1‑106 (MQ=255)
                                                                          gTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGc                                                                                                                   <  4:666636/106‑1 (MQ=255)
                                                                          gTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGc                                                                        <  4:283281/149‑1 (MQ=255)
                                                                           tGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATcc                                                                                                   <  2:11443/121‑1 (MQ=255)
                                                                           tGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATcc                                                                                                   >  1:11443/1‑121 (MQ=255)
                                                                             aagaaTTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGaaa                                                                                                                           >  4:474717/1‑95 (MQ=255)
                                                                             aagaaTTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGaaa                                                                                                                           <  3:474717/95‑1 (MQ=255)
                                                                             aagaaTTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTcg                                                                                                               <  1:125180/107‑1 (MQ=255)
                                                                             aagaaTTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTcg                                                                                                               >  2:125180/1‑107 (MQ=255)
                                                                                 aTTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGc                                                                                           >  4:62991/1‑123 (MQ=255)
                                                                                 aTTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGc                                                                                           <  3:62991/123‑1 (MQ=255)
                                                                                       ggTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCg                                                           <  3:566056/149‑1 (MQ=255)
                                                                                         tCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCgg                                                          <  4:386190/148‑1 (MQ=255)
                                                                                         tCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCgg                                                          >  3:386190/1‑148 (MQ=255)
                                                                                         tCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGt                                                         >  1:260110/1‑149 (MQ=255)
                                                                                         tCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGt                                                         >  3:547763/1‑149 (MQ=255)
                                                                                           aTAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGa                                                       <  3:417005/149‑1 (MQ=255)
                                                                                             aGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAg                                                                          >  4:646541/1‑128 (MQ=255)
                                                                                             aGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAg                                                                          <  3:646541/128‑1 (MQ=255)
                                                                                               aTCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAAc                                                                                                        <  4:640879/96‑1 (MQ=255)
                                                                                               aTCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAAc                                                                                                        >  3:640879/1‑96 (MQ=255)
                                                                                                tCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACtt                                                  <  3:32707/149‑1 (MQ=255)
                                                                                                   cGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTAc                                               <  3:340045/149‑1 (MQ=255)
                                                                                                    gTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTgg                                                                <  3:217618/131‑1 (MQ=255)
                                                                                                    gTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTgg                                                                >  4:217618/1‑131 (MQ=255)
                                                                                                    gTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTgg                                                                <  3:620701/131‑1 (MQ=255)
                                                                                                    gTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTgg                                                                >  4:620701/1‑131 (MQ=255)
                                                                                                    gTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACg                                              >  3:250859/1‑149 (MQ=255)
                                                                                                     tCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACgg                                             >  1:532397/1‑149 (MQ=255)
                                                                                                            aagaagAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACAt                                      <  1:314270/149‑1 (MQ=255)
                                                                                                              gaagaGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTcgc                                                                                                              <  2:137801/75‑1 (MQ=255)
                                                                                                              gaagaGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTcgc                                                                                                              >  1:137801/1‑75 (MQ=255)
                                                                                                                agagGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTg                                  <  4:250859/149‑1 (MQ=255)
                                                                                                                 gagGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACg                                              >  2:623070/1‑136 (MQ=255)
                                                                                                                 gagGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACg                                              <  1:623070/136‑1 (MQ=255)
                                                                                                                   gggAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTa                               <  2:87523/149‑1 (MQ=255)
                                                                                                                    ggAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTAc                              >  4:411028/1‑149 (MQ=255)
                                                                                                                       aGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCa                           <  3:293884/149‑1 (MQ=255)
                                                                                                                        gACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAg                                                              >  1:621052/1‑113 (MQ=255)
                                                                                                                        gACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAg                                                              <  2:621052/113‑1 (MQ=255)
                                                                                                                        gACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAc                          >  4:602294/1‑149 (MQ=255)
                                                                                                                           aacaTTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGc                       >  1:88521/1‑149 (MQ=255)
                                                                                                                            acaTTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCa                      >  2:291874/1‑149 (MQ=255)
                                                                                                                             caTTAGCAAGGTTGTGTACATTGGTATACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAACCTTTACGGAATACATGCTGTTACGCACCGCaa                     <  1:232690/149‑1 (MQ=255)
                                                                                                                              aTTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCaaa                    >  2:281624/1‑149 (MQ=255)
                                                                                                                                      aattGTGTACAAATGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTCACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTAc            >  1:576687/3‑149 (MQ=255)
                                                                                                                                        ttGTGTACAAATGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACg                                              <  1:583113/113‑1 (MQ=255)
                                                                                                                                        ttGTGTACAAATGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACg                                              >  2:583113/1‑113 (MQ=255)
                                                                                                                                        ttGTGTACAAATGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTAccc          >  3:663690/1‑149 (MQ=255)
                                                                                                                                           tgtACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTg       <  3:478016/149‑1 (MQ=255)
                                                                                                                                             tACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTa                                                                               <  4:87471/75‑1 (MQ=255)
                                                                                                                                             tACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTa                                                                               >  3:87471/1‑75 (MQ=255)
                                                                                                                                             tACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGcc     >  4:214106/1‑149 (MQ=255)
                                                                                                                                               cATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCgg   <  1:295581/149‑1 (MQ=255)
                                                                                                                                                aTTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCggg  >  1:217527/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTGCCGGG  >  NC_000913/887247‑887539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: