Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,041,937 T→G 11.3% intergenic (+22/+93) appA → / ← etk phosphoanhydride phosphorylase/tyrosine‑protein kinase, role in O‑antigen capsule formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,041,9370TG11.3% 161.2 / 14.4 53intergenic (+22/+93)appA/etkphosphoanhydride phosphorylase/tyrosine‑protein kinase, role in O‑antigen capsule formation
Reads supporting (aligned to +/- strand):  ref base T (24/23);  new base G (3/3);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

GCCGCCCGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGG  >  NC_000913/1041792‑1042085
                                                                                                                                                 |                                                                                                                                                    
gccgccCGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTAc                                                                                                                                                   >  4:207785/1‑149 (MQ=255)
gccgccCGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTAc                                                                                                                                                   <  1:582772/149‑1 (MQ=255)
  cgccCGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCt                                                                                                                                                 <  1:92265/149‑1 (MQ=255)
      cGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTGATGCATAAAAAAGAGCATTCAGTTACCTGAAt                                                                                                                                             <  2:217561/149‑1 (MQ=255)
        gagaGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGGTAACTGAATGc                                                                                                                                           <  2:117281/149‑1 (MQ=255)
           agGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGctct                                                                                                                                        <  3:459268/149‑1 (MQ=255)
             gTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGaa                                                                                                                                              >  3:490703/1‑141 (MQ=255)
             gTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGaa                                                                                                                                              <  4:490703/141‑1 (MQ=255)
             gTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGa                                                                                                                                      >  4:160284/1‑149 (MQ=255)
             gTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGa                                                                                                                                      >  4:649833/1‑149 (MQ=255)
              tGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAAt                                                                                                                                             >  3:442239/1‑141 (MQ=255)
              tGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAAt                                                                                                                                             <  4:442239/141‑1 (MQ=255)
               gAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAgg                                                                                                                                    >  3:625980/1‑149 (MQ=255)
                    tGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCtgat                                                                                                                               <  3:207785/149‑1 (MQ=255)
                    tGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCtgat                                                                                                                               <  3:649833/149‑1 (MQ=255)
                    tGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCtgat                                                                                                                               <  2:554097/149‑1 (MQ=255)
                          tGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACaaa                                                                                                                         <  4:483030/149‑1 (MQ=255)
                             cAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACga                                                                                                                      <  1:217841/149‑1 (MQ=255)
                                    tgAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTg                                                                                                               >  1:367191/1‑149 (MQ=255)
                                          gCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACGAACGAAGAACTGTCTAAt                                                                                                         >  1:508777/1‑149 (MQ=255)
                                          gCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAAt                                                                                                         <  4:328040/149‑1 (MQ=255)
                                          gCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAAt                                                                                                         <  3:124060/149‑1 (MQ=255)
                                                               ttCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAg                                                                                         >  3:687010/1‑144 (MQ=255)
                                                               ttCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAg                                                                                         <  4:687010/144‑1 (MQ=255)
                                                                          gTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATc                                                                         >  3:67381/1‑149 (MQ=255)
                                                                           ttttACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATcc                                                                        >  2:484027/1‑149 (MQ=255)
                                                                                 gCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCAc                                                                  >  3:606758/1‑149 (MQ=255)
                                                                                   aaaTCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATg                                                                                                        <  2:623816/109‑1 (MQ=255)
                                                                                   aaaTCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATg                                                                                                        >  1:623816/1‑109 (MQ=255)
                                                                                      tCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCa                                                             >  2:21136/1‑149 (MQ=255)
                                                                                        gTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGt                                                           >  2:497592/1‑149 (MQ=255)
                                                                                                aGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTActct                                                   >  3:39312/1‑149 (MQ=255)
                                                                                                     gCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTtctc                                              <  4:625980/149‑1 (MQ=255)
                                                                                                             gCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAAc                                      <  3:160284/149‑1 (MQ=255)
                                                                                                             gCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCACGGAGTAAc                                      >  1:349078/1‑149 (MQ=255)
                                                                                                               gTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACta                                    <  1:99883/149‑1 (MQ=255)
                                                                                                                    gTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAAcc                               >  2:506410/1‑149 (MQ=255)
                                                                                                                     tttGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCtga                                                                                                                                >  1:660052/1‑51 (MQ=255)
                                                                                                                     tttGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCtga                                                                                                                                <  2:660052/51‑1 (MQ=255)
                                                                                                                           aTGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCg                                             >  1:75531/1‑128 (MQ=255)
                                                                                                                           aTGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCg                                             <  2:75531/128‑1 (MQ=255)
                                                                                                                            tGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGt                       <  4:67381/149‑1 (MQ=255)
                                                                                                                             gCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGtt                      >  1:599520/1‑149 (MQ=255)
                                                                                                                                 aaaaaaGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAg                  >  1:86567/1‑149 (MQ=255)
                                                                                                                                    tcagagCATTCAGGTAACTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGt                                                           >  2:300766/3‑105 (MQ=255)
                                                                                                                                    tcagagCATTCAGGTAACTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGt                                                           <  1:300766/103‑1 (MQ=255)
                                                                                                                                      agagCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTa             >  2:133112/1‑149 (MQ=255)
                                                                                                                                       gagCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTaa            <  4:606758/149‑1 (MQ=255)
                                                                                                                                       gagCATTCAGGTAACTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTaa            >  3:466576/1‑149 (MQ=255)
                                                                                                                                        agCATTCAGGTAACTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAAc           <  4:466576/149‑1 (MQ=255)
                                                                                                                                          cATTCAGGTAACTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTg         >  3:285341/1‑149 (MQ=255)
                                                                                                                                            ttCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTaa                           <  1:359325/129‑1 (MQ=255)
                                                                                                                                            ttCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTaa                           >  2:359325/1‑129 (MQ=255)
                                                                                                                                                 ttACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCgg  <  1:484027/149‑1 (MQ=255)
                                                                                                                                                 ttACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCgg  >  1:132507/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCCGCCCGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGG  >  NC_000913/1041792‑1042085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑