| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,130,178 | G→A | 6.5% | intergenic (‑50/+26) | flgM ← / ← flgA | anti‑sigma factor for FliA (sigma 28)/assembly protein for flagellar basal‑body periplasmic P ring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,130,178 | 0 | G | A | 6.5% | 188.9 / 3.3 | 61 | intergenic (‑50/+26) | flgM/flgA | anti‑sigma factor for FliA (sigma 28)/assembly protein for flagellar basal‑body periplasmic P ring |
| Reads supporting (aligned to +/- strand): ref base G (31/26); new base A (2/2); total (33/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01 | |||||||||||
TTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTT > NC_000913/1130034‑1130326 | tttGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAg < 2:634267/149‑1 (MQ=255) cgccCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGACAAGGGACg > 2:348780/1‑149 (MQ=255) cgccCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGACAAGGGACg > 4:112112/1‑149 (MQ=255) gccCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACgg < 3:681509/149‑1 (MQ=255) gccCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACgg < 1:512785/149‑1 (MQ=255) gTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCtt > 1:363380/1‑149 (MQ=255) gTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCtt > 3:220303/1‑149 (MQ=255) cGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTaa < 4:683572/149‑1 (MQ=255) ttACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACa > 2:524102/1‑149 (MQ=255) ttACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACa < 3:596795/149‑1 (MQ=255) aCCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTaa > 3:332882/1‑145 (MQ=255) aCCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTaa < 4:332882/145‑1 (MQ=255) ggCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGTCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTa > 1:552906/1‑149 (MQ=255) tCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGtt > 3:192418/1‑149 (MQ=255) tCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGtt > 4:548582/1‑149 (MQ=255) tCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGCAATCTTTAACAGCTTACAGGtt > 1:593944/1‑149 (MQ=255) tGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTtata < 2:552906/149‑1 (MQ=255) tttCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGa > 4:644975/1‑149 (MQ=255) gcgcgGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAatat < 4:220303/149‑1 (MQ=255) gTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCa < 4:192418/149‑1 (MQ=255) tGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATc < 3:548582/149‑1 (MQ=255) gTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCaa > 1:284828/1‑94 (MQ=255) gTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCaa < 2:284828/94‑1 (MQ=255) gTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATc > 1:54283/1‑149 (MQ=255) aGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAAcgc > 3:472974/1‑149 (MQ=255) aGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAAcgc > 2:313185/1‑149 (MQ=255) gCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACa < 1:21972/104‑1 (MQ=255) gCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACa > 2:21972/1‑104 (MQ=255) ttCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCt > 4:288253/1‑105 (MQ=255) ttCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCt < 3:288253/105‑1 (MQ=255) gCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTAcc < 2:54283/149‑1 (MQ=255) gTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTcc > 2:221857/1‑149 (MQ=255) gATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGAt < 2:363380/149‑1 (MQ=255) aTCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATa < 4:472974/149‑1 (MQ=255) tACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATg > 2:41450/1‑149 (MQ=255) aCTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATTGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATga < 2:355603/149‑2 (MQ=255) aCTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATgc < 1:371257/149‑1 (MQ=255) tCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTa > 3:300068/1‑129 (MQ=255) tCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTa < 4:300068/129‑1 (MQ=255) gTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCcg > 4:393098/1‑149 (MQ=255) gTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCcg > 3:612222/1‑149 (MQ=255) tCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGACAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCAtt < 3:112112/149‑1 (MQ=255) tCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGACAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCAtt < 1:348780/149‑1 (MQ=255) tCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCtg > 2:321958/1‑149 (MQ=255) cATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCtgt < 3:666804/149‑1 (MQ=255) aTTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTTCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCtgtg > 3:33707/1‑149 (MQ=255) ttGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATcaa < 1:33784/97‑1 (MQ=255) ttGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATcaa > 2:33784/1‑97 (MQ=255) ttGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGc < 4:612222/149‑1 (MQ=255) ttttATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCa > 3:157464/1‑149 (MQ=255) tCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGtt < 1:524102/149‑1 (MQ=255) aTGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCa > 1:453521/1‑149 (MQ=255) tgtgTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAg > 3:614358/1‑149 (MQ=255) gCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTg > 1:611376/1‑149 (MQ=255) cTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGa > 3:605376/1‑149 (MQ=255) ttatttatCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGAc > 1:495333/1‑149 (MQ=255) ttatttatCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGAc > 4:117772/1‑149 (MQ=255) ttatttatCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGAc > 2:67402/1‑149 (MQ=255) ttatttatCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGAc < 3:393098/149‑1 (MQ=255) tttatCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGCATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACttt < 4:614358/149‑1 (MQ=255) ttatCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTc < 2:453521/149‑1 (MQ=255) gCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGtt > 3:10465/1‑149 (MQ=255) | TTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTT > NC_000913/1130034‑1130326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |