Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,248,064 | T→A | 8.6% | K351* (AAA→TAA) | dhaM ← | putative dihydroxyacetone‑specific PTS enzymes: HPr, EI components |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,248,064 | 0 | T | A | 8.6% | 309.3 / 16.8 | 93 | K351* (AAA→TAA) | dhaM | putative dihydroxyacetone‑specific PTS enzymes: HPr, EI components |
Reads supporting (aligned to +/- strand): ref base T (45/40); new base A (3/5); total (48/45) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.77e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01 |
TAGAATAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTC > NC_000913/1247918‑1248212 | tAGAATAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTaa < 4:599800/149‑1 (MQ=255) aaTAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGaa > 3:300946/1‑149 (MQ=255) aaTAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGaa < 3:202222/149‑1 (MQ=255) ttGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGaa > 4:216043/1‑144 (MQ=255) ttGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGaa < 3:216043/144‑1 (MQ=255) ttGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTtgc < 4:300946/149‑1 (MQ=255) ttGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGAACttag < 4:290704/149‑3 (MQ=255) tGGCGAGTTAAACTGCGGGAGTTCTTCTCTCGTTTGGGTTAGGTAGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGAACttagc < 3:316777/149‑4 (MQ=255) ggCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGACATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTtgctg < 1:84216/149‑1 (MQ=255) gTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAgg > 1:226006/1‑149 (MQ=255) gTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAgg < 2:278818/149‑1 (MQ=255) aaaCTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACtt < 1:130487/137‑1 (MQ=255) aaaCTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACtt > 2:130487/1‑137 (MQ=255) aaaCTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCat > 3:446118/1‑149 (MQ=255) aCTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCatat > 4:665900/1‑149 (MQ=255) ggAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGATCTTGCTGCCAGGCATATTCTGcc < 4:421061/149‑1 (MQ=255) gAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGcc > 4:216165/1‑148 (MQ=255) gAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGcc < 3:216165/148‑1 (MQ=255) cttcatTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCaa > 4:519485/1‑149 (MQ=255) ttcttTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAAt > 1:28285/1‑149 (MQ=255) tttCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGtt > 4:541209/1‑149 (MQ=255) gTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATg < 3:468377/149‑1 (MQ=255) tttGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCa > 1:453604/1‑121 (MQ=255) tttGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCa < 2:453604/121‑1 (MQ=255) tttGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGc < 1:457752/149‑1 (MQ=255) tttGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGc > 3:221413/1‑149 (MQ=255) tCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAgg > 4:18438/1‑149 (MQ=255) cAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGa < 2:302531/149‑1 (MQ=255) gTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGtt > 1:288604/1‑149 (MQ=255) gTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGtt > 1:142370/1‑149 (MQ=255) gTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGtt < 2:618214/149‑1 (MQ=255) ggACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCg > 1:443443/1‑149 (MQ=255) gACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGc < 1:548868/149‑1 (MQ=255) aCCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCt < 3:476858/149‑1 (MQ=255) cAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTg > 3:547790/1‑149 (MQ=255) gATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTACTGGCTGAGTTCTTTAAGAACTTGCTGCCAGGCATATTCAGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTgccgccgcc > 4:448347/1‑149 (MQ=255) aTGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCca < 3:541209/149‑1 (MQ=255) cAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCcagca > 2:237850/1‑149 (MQ=255) gaagaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAGGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGt > 1:636072/1‑149 (MQ=255) gaagaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGt > 1:189752/1‑149 (MQ=255) gaagaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGt > 3:169718/1‑149 (MQ=255) gaagaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGt > 2:661318/1‑149 (MQ=255) gaagaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGAACtta > 2:73879/1‑84 (MQ=255) gaagaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGAACtta < 1:73879/85‑2 (MQ=255) gaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTc < 1:490159/127‑1 (MQ=255) gaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTc > 2:490159/1‑127 (MQ=255) gaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTcc < 1:226121/149‑1 (MQ=255) gaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTcc > 4:498978/1‑149 (MQ=255) gaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTcc > 2:650724/1‑149 (MQ=255) gaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTcc > 4:653057/1‑149 (MQ=255) cGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGAAGTTCCGGa < 2:522798/149‑1 (MQ=255) gTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGat > 1:563080/1‑149 (MQ=255) acaTCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGatcatc < 1:272032/149‑1 (MQ=255) aTCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAg < 2:80755/111‑1 (MQ=255) aTCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAg > 1:80755/1‑111 (MQ=255) aTCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTa > 1:476825/1‑149 (MQ=255) aaTATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACa < 2:288604/149‑1 (MQ=255) atatAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAg < 2:476825/149‑1 (MQ=255) taGCGTGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAgtgt < 4:446118/149‑1 (MQ=255) cGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATg < 3:653057/149‑1 (MQ=255) aGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGt < 3:57561/149‑1 (MQ=255) gCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTg < 1:650724/149‑1 (MQ=255) ttGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGAc < 3:651587/149‑1 (MQ=255) tAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGt < 1:278009/82‑1 (MQ=255) tAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGt > 2:278009/1‑82 (MQ=255) aGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCttt < 3:208868/44‑1 (MQ=255) aGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCttt > 4:208868/1‑44 (MQ=255) aGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGa > 3:228527/1‑149 (MQ=255) tatTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGaaaa < 3:665900/149‑1 (MQ=255) atTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGat < 2:130168/120‑1 (MQ=255) atTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGat > 1:130168/1‑120 (MQ=255) atTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAg > 3:186252/1‑149 (MQ=255) ttCATCAGCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAGTGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGa > 2:372769/1‑149 (MQ=255) catcatCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGAtt > 2:381968/1‑149 (MQ=255) tcatcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTg > 4:597341/1‑134 (MQ=255) tcatcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTg < 3:597341/134‑1 (MQ=255) atcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCgg < 1:45772/149‑1 (MQ=255) atcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCgg > 1:631040/1‑149 (MQ=255) atcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGATGACCAGAAAAGATTGCgg > 1:52490/1‑149 (MQ=255) cAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCaa > 3:688748/1‑149 (MQ=255) aGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAat < 1:237850/149‑1 (MQ=255) aGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAat < 1:661318/149‑1 (MQ=255) aGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAat < 2:142370/149‑1 (MQ=255) cTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGt > 2:463787/1‑149 (MQ=255) tGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTc > 4:504065/1‑149 (MQ=255) cTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGc > 1:202148/1‑149 (MQ=255) agcaaGTTCTTAAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTgg > 4:461204/4‑149 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCt > 2:151575/1‑149 (MQ=255) caaGTTCTTAAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGAcc > 3:665098/2‑112 (MQ=255) caaGTTCTTAAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGAcc < 4:665098/111‑1 (MQ=255) aGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTc < 4:221413/149‑1 (MQ=255) aGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTc < 2:28285/149‑1 (MQ=255) aGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTc < 1:381968/149‑1 (MQ=255) aGTTCTTAAAGAACTTGCTGCCAGGCATATTCTGCCTTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTc < 3:461204/149‑1 (MQ=255) ttCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTg > 2:667287/1‑149 (MQ=255) tttAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCtt > 1:418040/1‑149 (MQ=255) tAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAGTGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTc < 1:372769/149‑1 (MQ=255) | TAGAATAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTC > NC_000913/1247918‑1248212 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |