| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,256,650 | A→G | 6.6% | intergenic (‑698/+71) | ycgV ← / ← ychF | putative adhesin/catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,256,650 | 0 | A | G | 6.6% | 190.5 / 2.7 | 60 | intergenic (‑698/+71) | ycgV/ychF | putative adhesin/catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated |
| Reads supporting (aligned to +/- strand): ref base A (28/28); new base G (2/2); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
AACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGC > NC_000913/1256503‑1256798 | aaCTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCaa > 4:221564/1‑149 (MQ=255) aCTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAAt > 4:240847/1‑149 (MQ=255) cTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAAtt < 4:363420/149‑1 (MQ=255) aCGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCGATTGCg > 3:8583/1‑149 (MQ=255) cGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGt < 2:54713/149‑1 (MQ=255) cGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGt < 1:456891/149‑1 (MQ=255) tCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGAtttt > 3:636977/1‑149 (MQ=255) cACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTta < 1:308434/149‑1 (MQ=255) cACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTta < 3:221564/149‑1 (MQ=255) tGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTtatat > 2:153946/1‑149 (MQ=255) cTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCGATTGCGTGGATTTTATATAc < 4:8583/149‑1 (MQ=255) tAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACt > 2:610181/1‑149 (MQ=255) aTGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTta > 4:361337/1‑141 (MQ=255) aTGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTta < 3:361337/141‑1 (MQ=255) tCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGctct > 3:283149/1‑149 (MQ=255) ggCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGAt < 4:71003/126‑1 (MQ=255) ggCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGAt > 3:71003/1‑126 (MQ=255) tCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAACTCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATgag > 1:202411/1‑149 (MQ=255) tCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTta > 4:182659/1‑126 (MQ=255) tCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTta < 3:182659/126‑1 (MQ=255) tGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGAt < 4:636977/149‑1 (MQ=255) gAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGAtt > 2:263227/1‑149 (MQ=255) gAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGAtt > 4:236456/1‑149 (MQ=255) cacTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCGATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAg > 1:27965/1‑149 (MQ=255) cacTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAg < 3:240847/149‑1 (MQ=255) tCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCt > 1:561573/1‑149 (MQ=255) tttAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCa > 4:536360/1‑149 (MQ=255) tAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATg < 3:536360/149‑1 (MQ=255) aaTTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATga < 3:236456/149‑1 (MQ=255) attattCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAACATCCACGCAATTGCGTGGATTTTATATACTTTTgcgc < 4:532203/119‑1 (MQ=255) attattCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTgcgc > 3:532203/1‑119 (MQ=255) attattCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATgag < 2:202411/149‑1 (MQ=255) ttattCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATgaga < 1:610181/149‑1 (MQ=255) ttCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACaa < 1:71168/149‑1 (MQ=255) tcGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGa > 3:453931/1‑94 (MQ=255) tcGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGa < 4:453931/94‑1 (MQ=255) tcGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATaa > 4:123019/1‑149 (MQ=255) gTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAaat < 4:283149/149‑1 (MQ=255) gTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTa < 1:666871/122‑1 (MQ=255) gTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTa > 2:666871/1‑122 (MQ=255) cTATAGGCAATTAGCACAAAAGTGTAGGATCTTACAAGAATGATTAGGACTCGGTGCAATGAAAAATCCACGCAATTGCTTGGATTTTATATACTTTTGGGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGa < 4:426929/149‑1 (MQ=255) cTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGAttt < 3:133006/87‑1 (MQ=255) cTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGAttt > 4:133006/1‑87 (MQ=255) cTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAaattaa < 3:625861/144‑1 (MQ=255) cTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAaattaa > 4:625861/1‑144 (MQ=255) tataGGCAATAAGCACAAAAGTGTAGGATGTGACAAGAACGATAAGGACTCGGTGAAATGAATAATCCACGCAATTACGTGGATTTAATATACTTTTGCGCTCTTCATGAGATTTAGAGTAACCTCATGAGACACTAAATTAATTAGAc > 3:426929/1‑149 (MQ=255) gCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATga < 1:292517/105‑1 (MQ=255) gCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATga > 2:292517/1‑105 (MQ=255) aaGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAgg > 1:459379/1‑149 (MQ=255) aaaGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTtca < 1:153946/149‑1 (MQ=255) aGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTtcatc < 1:263227/149‑1 (MQ=255) tACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCt < 2:561573/149‑1 (MQ=255) cGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTAc < 3:123019/149‑1 (MQ=255) ggTGAAATGAAAAATCCACACAATTGCGTGGATTTTATGTACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATATTTAcc > 4:326663/1‑149 (MQ=255) gaaaAAGCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCAc > 2:491433/1‑149 (MQ=255) aaaaTCCACGCGATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGc < 2:27965/149‑1 (MQ=255) aTCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCAtt > 1:681868/1‑149 (MQ=255) tCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCAttt > 3:469805/1‑149 (MQ=255) cACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTg > 2:647114/1‑149 (MQ=255) cGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAaattaat < 1:572016/75‑1 (MQ=255) cGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAaattaat > 2:572016/1‑75 (MQ=255) aaTTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATTGCCATCTTTCACGACGTAATCTTTACCTTCTGCACGCATTTTGCCTGc > 4:326059/1‑149 (MQ=255) | AACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTTAATTATTCTCGTGGTTCACTATAGGCAATAAGCACAAAAGTGTAGGATGTTACAAGAATGATTAGGACTCGGTGAAATGAAAAATCCACGCAATTGCGTGGATTTTATATACTTTTGCGCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGC > NC_000913/1256503‑1256798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |