Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,304,474 T→A 5.2% I214N (ATT→AAT)  oppC → oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,304,4740TA 5.2% 398.2 / 7.7 113I214N (ATT→AAT) oppColigopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (50/57);  new base A (4/2);  total (54/59)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 113 total aligned reads displayed.

GCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGATGGCGC  >  NC_000913/1304328‑1304618
                                                                                                                                                  |                                                                                                                                                
gctgATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATtg                                                                                                                                                >  4:467490/1‑149 (MQ=255)
   gATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGc                                                                                                                                             <  2:600490/149‑1 (MQ=255)
       ttCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGaa                                                                                                                                         <  2:672731/149‑1 (MQ=255)
               gATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCg                                                                                                                                 <  4:458187/149‑1 (MQ=255)
                aTAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCgg                                                                                                                                >  1:542865/1‑149 (MQ=255)
                   ggCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGgtgt                                                                                                                             <  3:263463/149‑1 (MQ=255)
                    gCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGGCACAATGTGGCGAAtaacaat                                                                                                                                  >  2:648252/1‑137 (MQ=255)
                         gTTCCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTgtggtggt                                                                                                                       >  2:450217/1‑149 (MQ=255)
                          tttCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTgtggtggtg                                                                                                                      <  3:166885/149‑1 (MQ=255)
                               tggctggATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTa                                                                                                                 >  3:185850/1‑149 (MQ=255)
                                ggctggATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTAc                                                                                                                <  3:390484/149‑1 (MQ=255)
                                ggctggATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTAc                                                                                                                >  1:301730/1‑149 (MQ=255)
                                  ctggATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGc                                                                                                              <  2:2842/149‑1 (MQ=255)
                                     gATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATc                                                                                                           >  3:310219/1‑149 (MQ=255)
                                     gATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATc                                                                                                           <  2:220082/149‑1 (MQ=255)
                                          ggCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTc                                                                                                      <  2:639315/149‑1 (MQ=255)
                                           gCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCt                                                                                                     >  1:78527/1‑149 (MQ=255)
                                            cTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTg                                                                                                    <  2:225093/149‑1 (MQ=255)
                                            cTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTATGCCGAATGTACTCGGTGTGGTGGTGGTc                                                                                                                   >  2:648196/1‑134 (MQ=255)
                                            cTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTATGCCGAATGTACTCGGTGTGGTGGTGGTc                                                                                                                   <  1:648196/134‑1 (MQ=255)
                                             tCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTgg                                                                                                   >  2:327743/1‑149 (MQ=255)
                                                 aTTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGcc                                                                                               <  1:327743/149‑1 (MQ=255)
                                                  ttGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGccc                                                                                              >  1:457481/1‑149 (MQ=255)
                                                    gtgCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAg                                                                                            >  1:73218/1‑149 (MQ=255)
                                                      gCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGGGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCa                                                                                          >  2:101331/1‑149 (MQ=255)
                                                      gCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTa                                                                                                                 <  4:563841/126‑1 (MQ=255)
                                                         tGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGa                                                                                       <  2:542865/149‑1 (MQ=255)
                                                           ggCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATc                                                                                     <  2:301730/149‑1 (MQ=255)
                                                              aaaCCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCctc                                                                                  >  4:564217/1‑149 (MQ=255)
                                                               aaCCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCctct                                                                                 <  2:78527/149‑1 (MQ=255)
                                                                aCCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCtt                                                                                <  3:164594/149‑1 (MQ=255)
                                                                aCCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCACCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCtt                                                                                <  4:565892/149‑1 (MQ=255)
                                                                 cccTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCttt                                                                               >  2:632180/1‑149 (MQ=255)
                                                                  ccTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTg                                                                              >  1:357263/1‑149 (MQ=255)
                                                                   cTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGa                                                                             <  1:616971/149‑1 (MQ=255)
                                                                     gAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCGGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAAt                                                                           <  1:91949/149‑1 (MQ=255)
                                                                     gAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAAt                                                                           <  4:498354/149‑1 (MQ=255)
                                                                       gTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCt                                                                         >  2:540793/1‑149 (MQ=255)
                                                                        tCTGAAGCGCAAAGAGTCTATTGAGGCGGCACAAGTTGGTGGTGTATCGTCATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCtt                                                                        <  4:583043/149‑1 (MQ=255)
                                                                          tGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTc                                                                      <  4:216729/149‑1 (MQ=255)
                                                                            aaGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCt                                                                    <  4:627795/149‑1 (MQ=255)
                                                                            aaGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCt                                                                    <  3:82391/149‑1 (MQ=255)
                                                                             aGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCtt                                                                   >  1:15203/1‑149 (MQ=255)
                                                                              gcgcAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTa                                                                  <  2:15203/149‑1 (MQ=255)
                                                                              gcgcAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTa                                                                  <  3:467490/149‑1 (MQ=255)
                                                                                  aaaGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCtt                                                                   >  4:285072/1‑144 (MQ=255)
                                                                                  aaaGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCtt                                                                   <  3:285072/144‑1 (MQ=255)
                                                                                  aaaGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCtt                                                              >  2:436521/1‑149 (MQ=255)
                                                                                  aaaGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCtt                                                              <  1:540793/149‑1 (MQ=255)
                                                                                   aaGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTc                                                             >  3:176840/1‑149 (MQ=255)
                                                                                        tttATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTTGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTggg                                                        >  4:569340/1‑149 (MQ=255)
                                                                                          tATTGAGGCGGCACAAGATGGCGGTGTATCGACATCGGGCAATGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGTATCACTTCTGGTGCCCAGCATGATCATCTTTGAATCTTTCCTTAGCTTCCTgggtt                                                      >  4:426991/1‑147 (MQ=255)
                                                                                           aTTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTc                                                                                                                                  >  3:522850/1‑72 (MQ=255)
                                                                                           aTTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTc                                                                                                                                  <  4:522850/72‑1 (MQ=255)
                                                                                             tGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTgg                                                   <  2:66368/149‑1 (MQ=255)
                                                                                              gAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTggg                                                  >  4:139568/1‑149 (MQ=255)
                                                                                                    gcACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCa                                            >  2:579603/1‑149 (MQ=255)
                                                                                                      acaAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCGGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAag                                          >  3:149937/1‑149 (MQ=255)
                                                                                                      acaAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGt                                                      <  1:345858/137‑1 (MQ=255)
                                                                                                      acaAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGt                                                      >  2:345858/1‑137 (MQ=255)
                                                                                                      acaAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAag                                          >  1:120702/1‑149 (MQ=255)
                                                                                                      acaAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAag                                          >  1:671778/1‑149 (MQ=255)
                                                                                                      acaAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAag                                          >  2:309475/1‑149 (MQ=255)
                                                                                                        aaGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAagag                                        >  1:365627/1‑149 (MQ=255)
                                                                                                          gTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGcc                                      <  1:450217/149‑1 (MQ=255)
                                                                                                            tGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGt                                    <  4:102745/149‑1 (MQ=255)
                                                                                                            tGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGt                                    >  1:226058/1‑149 (MQ=255)
                                                                                                            tGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGt                                    <  2:365627/149‑1 (MQ=255)
                                                                                                               cGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTaa                                 <  4:234562/149‑1 (MQ=255)
                                                                                                                ggTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAag                                <  1:632180/149‑1 (MQ=255)
                                                                                                                 gtgtATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGt                                    <  2:606892/144‑1 (MQ=255)
                                                                                                                 gtgtATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGt                                    >  1:606892/1‑144 (MQ=255)
                                                                                                                     aTCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCt                           >  3:53881/1‑149 (MQ=255)
                                                                                                                      tCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTg                          >  1:89675/1‑149 (MQ=255)
                                                                                                                      tCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTg                          <  3:58307/149‑1 (MQ=255)
                                                                                                                      tCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTg                          >  1:294015/1‑149 (MQ=255)
                                                                                                                       cGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTgg                         >  1:353320/1‑149 (MQ=255)
                                                                                                                         aCATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTg                                                          <  2:15581/114‑1 (MQ=255)
                                                                                                                         aCATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTg                                                          >  1:15581/1‑114 (MQ=255)
                                                                                                                               ggCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTCAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCAtt                 >  4:324278/1‑149 (MQ=255)
                                                                                                                                  aTTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCt              <  2:294015/149‑1 (MQ=255)
                                                                                                                                  aTTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCt              <  2:51017/149‑1 (MQ=255)
                                                                                                                                  aTTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCt              >  2:672142/1‑149 (MQ=255)
                                                                                                                                  aTTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGAATTGCt              >  2:604815/1‑149 (MQ=255)
                                                                                                                                  aTTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTCCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCt              <  1:2167/149‑1 (MQ=255)
                                                                                                                                    tGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGa            <  2:2790/149‑1 (MQ=255)
                                                                                                                                    tGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGa            <  2:353320/149‑1 (MQ=255)
                                                                                                                                    tGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGa            <  2:89675/149‑1 (MQ=255)
                                                                                                                                     tacaTTCGGCACAATGTGGCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGa            >  1:135960/4‑148 (MQ=255)
                                                                                                                                      ttgttCGCCACATTGTGCCGAATGTACTCGGTGAGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGt          >  2:130037/4‑149 (MQ=255)
                                                                                                                                        aTTCGGCACAATGTGGCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGa        >  2:238575/1‑149 (MQ=255)
                                                                                                                                         ttCGGCACAATGTGGCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGGTCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGAt       >  4:16395/1‑149 (MQ=255)
                                                                                                                                         ttCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGAt       <  1:254908/149‑1 (MQ=255)
                                                                                                                                          tCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGATg      <  1:309475/149‑1 (MQ=255)
                                                                                                                                          tCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGATg      <  2:671778/149‑1 (MQ=255)
                                                                                                                                           cGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGATgg     <  3:564217/149‑1 (MQ=255)
                                                                                                                                           cGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGATgg     >  1:238340/1‑149 (MQ=255)
                                                                                                                                              cacaTTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGATGgcgc  <  4:151508/149‑1 (MQ=255)
                                                                                                                                                 aTTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGAt       >  4:174059/1‑141 (MQ=255)
                                                                                                                                                 aTTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGAt       <  3:174059/141‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGATGGCGC  >  NC_000913/1304328‑1304618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: