| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,537,540 | G→A | 5.7% | A438A (GCC→GCT) | narY ← | nitrate reductase 2 (NRZ), beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,537,540 | 0 | G | A | 5.7% | 286.2 / 5.6 | 87 | A438A (GCC→GCT) | narY | nitrate reductase 2 (NRZ), beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (35/47); new base A (2/3); total (37/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
CCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGA > NC_000913/1537392‑1537685 | ccGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTgg < 3:567903/149‑1 (MQ=255)ccGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTgg < 2:665911/149‑1 (MQ=255) gAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATACCAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGc > 4:594143/1‑149 (MQ=255) gAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGACCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGc < 4:475585/149‑1 (MQ=255) aCCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGa < 3:655369/149‑1 (MQ=255) cAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAg < 2:54715/149‑1 (MQ=255) tCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTAc < 2:244306/149‑1 (MQ=255) gCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGAGCGTCGCCCAggtcgaaga < 1:224502/149‑8 (MQ=255) ccAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTtc > 1:502282/1‑149 (MQ=255) aaaGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTtcgt < 2:614215/149‑1 (MQ=255) aaGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCAAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTtcgtc < 3:242919/149‑1 (MQ=255) ggTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGa < 2:83106/149‑1 (MQ=255) gTAAAACTGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGAt < 3:594143/149‑1 (MQ=255) gTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGAt < 4:28978/149‑1 (MQ=255) aaaaCCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCGTAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATgg < 1:135261/149‑1 (MQ=255) aaaCCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGATCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGc > 1:445591/1‑149 (MQ=255) aaCCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGAt < 4:573883/145‑1 (MQ=255) aaCCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGAt > 3:573883/1‑145 (MQ=255) aCCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGAGCGTCGCCCAggtcgaa < 3:654547/135‑6 (MQ=255) aCCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGAGCGTCGCCCAggtcgaa > 4:654547/1‑130 (MQ=255) cGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGa < 3:53187/149‑1 (MQ=255) cGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGa < 3:490293/149‑1 (MQ=255) gCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGAGCGTCGCCCAggtc < 1:550790/129‑3 (MQ=255) gCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGAGCGTCGCCCAggtc > 2:550790/1‑127 (MQ=255) aGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTa > 4:265048/1‑149 (MQ=255) ccGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATc < 4:221935/149‑1 (MQ=255) ccGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATc < 1:53549/149‑1 (MQ=255) gCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTaa < 2:315002/149‑1 (MQ=255) gCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTc < 4:339191/149‑1 (MQ=255) cGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTcc < 3:200177/149‑1 (MQ=255) aTCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGttt > 2:5134/1‑149 (MQ=255) gcccgccATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTtgtg < 3:424130/149‑1 (MQ=255) cgccATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAAc > 4:346212/1‑149 (MQ=255) gccATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACg > 1:330094/1‑149 (MQ=255) cATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCa < 2:288976/149‑1 (MQ=255) aTTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCat < 3:425925/149‑1 (MQ=255) aTTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCat < 1:292249/149‑1 (MQ=255) tttCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCata < 2:330094/149‑1 (MQ=255) tttCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCata < 2:499372/149‑1 (MQ=255) ccGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGt < 3:119285/149‑1 (MQ=255) ccGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGt > 3:297041/1‑149 (MQ=255) cGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTg < 1:458909/149‑1 (MQ=255) tCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGcc < 2:505179/149‑1 (MQ=255) tCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGca < 2:143811/149‑2 (MQ=255) cGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCca < 2:47932/149‑1 (MQ=255) gATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTTTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCcatca > 4:464444/1‑149 (MQ=255) gATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTtcgt < 1:417729/83‑1 (MQ=255) gATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTtcgt > 2:417729/1‑83 (MQ=255) gATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCcatca > 3:546109/1‑149 (MQ=255) aaCAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATcc > 3:632919/1‑149 (MQ=255) aaCAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATcc > 1:156916/1‑149 (MQ=255) aaaCGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCGGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGttt > 2:591756/1‑149 (MQ=255) aaCGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATc > 3:295927/1‑134 (MQ=255) aaCGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATc < 4:295927/134‑1 (MQ=255) aCGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTGCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCa < 4:496941/149‑1 (MQ=255) aTCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAgcg > 2:406632/1‑149 (MQ=255) aTCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAgcg > 4:65952/1‑149 (MQ=255) tCTTCATAGTTGGCAATGGCGAGAAAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGAGGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGAGGCGCATCGCCATCATCCGTTTCAgcgc > 3:433425/1‑149 (MQ=255) ttCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGccc > 3:237453/1‑149 (MQ=255) ttCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGccc > 2:562895/1‑149 (MQ=255) tCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCa < 1:4990/97‑1 (MQ=255) tCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCa > 2:4990/1‑97 (MQ=255) ttGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTac > 3:642396/1‑149 (MQ=255) ttGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCATACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGAGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTac < 4:376966/149‑1 (MQ=255) cAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTaccgga < 3:261106/149‑1 (MQ=255) cAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTaccgga < 3:675798/149‑1 (MQ=255) tGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTaccggaccg < 3:511605/149‑1 (MQ=255) gCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGt < 4:618807/149‑1 (MQ=255) cGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTa > 3:33775/1‑149 (MQ=255) aGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCg < 3:346212/149‑1 (MQ=255) aCATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCTCCCGCAGTACCGGACCGGTATCGCCGGCACTc > 3:497112/1‑149 (MQ=255) tctcTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAAc > 2:39906/1‑149 (MQ=255) tctcTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAAc < 2:420696/149‑1 (MQ=255) tctTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTAt > 3:284269/1‑133 (MQ=255) tctTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTAt < 4:284269/133‑1 (MQ=255) tctTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACat > 4:553754/1‑149 (MQ=255) ttCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCcatc > 3:27658/1‑97 (MQ=255) ttCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCcatc < 4:27658/97‑1 (MQ=255) ttCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACatat > 2:557404/1‑149 (MQ=255) ttCGACCGGGGCGACGCTCAGCCCTCCTTCGACGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCAGATAGTGGCGCATACCCATCATACGTTTCAGCGTCCGCAATACCGGAGCGGTATCGCCCGCAGTCAACaaag > 2:197099/1‑146 (MQ=255) tCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATAtt < 2:156916/149‑1 (MQ=255) tCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATAtt < 4:237453/149‑1 (MQ=255) cGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTg > 1:288040/1‑149 (MQ=255) aCCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGc > 2:416935/1‑149 (MQ=255) ccTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCg > 3:688746/1‑149 (MQ=255) cTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGTGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCga < 3:265048/149‑1 (MQ=255) cTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGc < 2:398393/147‑1 (MQ=255) cTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGc > 1:398393/1‑147 (MQ=255) cTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCga < 1:557404/149‑1 (MQ=255) | CCGAACCGTGGCAACCGTCGCCAAAGGTAAAACCGCAGCCGTTGCGTTCTGCGAAGGCATCGCCCGCCATTTCCCGATGGCTGGTCGGGATAACAAAACGATCTTCATAGTTGGCAATGGCGAGATAGCGATACATCTCTTCGACCTGGGCGACGCTCAGGCCTACTTCGTCGATGGCACGAGTATCAGTAACGCCTTCCACGGTTTGTGAACGCATATAGTGGCGCATCGCCATCATCCGTTTCAGCGCCCGCAGTACCGGACCGGTATCGCCGGCACTCAACATATTGGCGA > NC_000913/1537392‑1537685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |