Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,546,625 C→A 8.3% G182V (GGA→GTA)  yddG ← aromatic amino acid exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,546,6250CA 8.3% 289.1 / 14.1 84G182V (GGA→GTA) yddGaromatic amino acid exporter
Reads supporting (aligned to +/- strand):  ref base C (40/37);  new base A (3/4);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-01

GCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGAC  >  NC_000913/1546478‑1546773
                                                                                                                                                   |                                                                                                                                                    
gcaTAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATcc                                                                                                                                                     <  3:300670/149‑1 (MQ=255)
       cAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCgcgtgcg                                                                                                                                              >  3:135655/1‑149 (MQ=255)
         aaTCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGta                                                                                                                                            >  2:150105/1‑149 (MQ=255)
           tCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGtatt                                                                                                                                          >  1:438792/1‑149 (MQ=255)
              tAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGtatttat                                                                                                                                       >  2:669946/1‑149 (MQ=255)
               aaGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGtatttatt                                                                                                                                      <  1:100756/149‑1 (MQ=255)
                aGGTAAATGCCTCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTc                                                                                                                                     <  2:294569/149‑1 (MQ=255)
                aGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTc                                                                                                                                     <  2:583059/149‑1 (MQ=255)
                 ggTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCg                                                                                                                                    >  4:362584/1‑149 (MQ=255)
                      aTGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACt                                                                                                                               >  2:552897/1‑149 (MQ=255)
                         ccGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACtgtg                                                                                                                            <  3:362584/149‑1 (MQ=255)
                             agagATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAAt                                                                                                                        >  1:332629/1‑149 (MQ=255)
                                      tttAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGccc                                                                                                               <  1:313194/149‑1 (MQ=255)
                                      tttAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGccc                                                                                                               >  2:574691/1‑149 (MQ=255)
                                      tttAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGccc                                                                                                               >  3:295868/1‑149 (MQ=255)
                                         aaTCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGa                                                                                                            >  2:377658/1‑149 (MQ=255)
                                             aTGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaaa                                                                                                        <  3:207414/149‑1 (MQ=255)
                                             aTGACGGGCGTCCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaaa                                                                                                        >  3:404575/1‑149 (MQ=255)
                                              tGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAAc                                                                                                       <  3:583181/149‑1 (MQ=255)
                                              tGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAAc                                                                                                       <  1:150105/149‑1 (MQ=255)
                                              tGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACg                                                                                                      >  3:61651/1‑149 (MQ=255)
                                                  gggCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAc                                                                                                   <  1:377658/149‑1 (MQ=255)
                                                  gggCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAc                                                                                                   <  1:574691/149‑1 (MQ=255)
                                                          tAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTGTTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc                                                                                           >  3:596169/1‑149 (MQ=255)
                                                              tatCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCa                                                                                       <  4:155347/149‑1 (MQ=255)
                                                                tCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAgg                                                                                     >  4:256271/1‑149 (MQ=255)
                                                                tCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAgg                                                                                     >  4:377065/1‑149 (MQ=255)
                                                                  aTTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGaa                                                                                   >  2:488165/1‑149 (MQ=255)
                                                                  aTTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATAcgca                                                                                                                                                  <  3:241889/86‑2 (MQ=255)
                                                                  aTTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATAcgca                                                                                                                                                  >  4:241889/1‑85 (MQ=255)
                                                                  aTTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATAcgca                                                                                                                                                  <  4:646363/86‑2 (MQ=255)
                                                                  aTTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATAcgca                                                                                                                                                  >  3:646363/1‑85 (MQ=255)
                                                                  aTTTCTGGCTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGaa                                                                                   >  2:53106/1‑149 (MQ=255)
                                                                    ttCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAAt                                                                                 <  2:332629/149‑1 (MQ=255)
                                                                    ttCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAAt                                                                                 >  2:656888/1‑149 (MQ=255)
                                                                      cTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATaa                                                                               <  1:177038/149‑1 (MQ=255)
                                                                      cTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATaa                                                                               <  1:552897/149‑1 (MQ=255)
                                                                       tGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAAc                                                                              >  1:69352/1‑149 (MQ=255)
                                                                            gtgGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATATGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAAt                                                                         <  1:53106/149‑1 (MQ=255)
                                                                                   aaGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAc                                                                                                   <  3:280775/116‑1 (MQ=255)
                                                                                   aaGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAc                                                                                                   >  4:280775/1‑116 (MQ=255)
                                                                                        aaTAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGgtggtg                                                             >  1:455152/1‑149 (MQ=255)
                                                                                          tAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGat                                                           >  4:6331/1‑149 (MQ=255)
                                                                                           aGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAAATCAATGGGCTGGTGGTGata                                                          <  2:455152/149‑1 (MQ=255)
                                                                                              aaaCCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGAtatta                                                       <  2:416642/149‑1 (MQ=255)
                                                                                               aaCCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGAtattat                                                      <  4:441354/149‑1 (MQ=255)
                                                                                                aCCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATggg                                                                      <  1:391905/132‑1 (MQ=255)
                                                                                                aCCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATggg                                                                      >  2:391905/1‑132 (MQ=255)
                                                                                                aCCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATattatt                                                     <  3:348960/149‑1 (MQ=255)
                                                                                                aCCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATattatt                                                     <  2:588098/149‑1 (MQ=255)
                                                                                                  ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtga                                                   >  2:367959/1‑149 (MQ=255)
                                                                                                  ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtga                                                   <  4:23900/149‑1 (MQ=255)
                                                                                                  ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGAGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtga                                                   <  3:295411/149‑1 (MQ=255)
                                                                                                   cacaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtgat                                                  >  2:32633/1‑149 (MQ=255)
                                                                                                    acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtgatg                                                 >  1:224208/1‑149 (MQ=255)
                                                                                                    acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtgatg                                                 >  1:115888/1‑149 (MQ=255)
                                                                                                       gACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGAtt                                              <  4:647923/149‑1 (MQ=255)
                                                                                                          ttGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTtca                                           <  3:6331/149‑1 (MQ=255)
                                                                                                             cTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtgat                                                  >  1:340136/1‑139 (MQ=255)
                                                                                                             cTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtgat                                                  <  2:340136/139‑1 (MQ=255)
                                                                                                             cTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTtcatca                                        <  3:377065/149‑1 (MQ=255)
                                                                                                              tCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTcatcat                                       >  3:137976/1‑149 (MQ=255)
                                                                                                              tCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTcatcat                                       >  2:660109/1‑149 (MQ=255)
                                                                                                                ccGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATaa                                     <  1:656888/149‑1 (MQ=255)
                                                                                                                        aGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTtcat                                          >  1:366247/1‑136 (MQ=255)
                                                                                                                        aGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAAccc                             >  1:477591/1‑149 (MQ=255)
                                                                                                                        aGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGAGAAACGCACCAATGAACACCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTtcat                                          <  2:366247/136‑1 (MQ=255)
                                                                                                                          gACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAg                                                                                      <  2:416606/90‑1 (MQ=255)
                                                                                                                          gACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAg                                                                                      >  1:416606/1‑90 (MQ=255)
                                                                                                                          gACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtga                                                   <  2:503211/125‑1 (MQ=255)
                                                                                                                          gACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATtga                                                   >  1:503211/1‑125 (MQ=255)
                                                                                                                           aCAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTtcatc                                         >  2:244391/1‑134 (MQ=255)
                                                                                                                           aCAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTtcatc                                         <  1:244391/134‑1 (MQ=255)
                                                                                                                              aaaaCGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCaa                                                                          >  2:184094/1‑98 (MQ=255)
                                                                                                                              aaaaCGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCaa                                                                          <  1:184094/98‑1 (MQ=255)
                                                                                                                                 aCGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCAcc                    <  4:404575/149‑1 (MQ=255)
                                                                                                                                     tAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCt                <  2:69352/149‑1 (MQ=255)
                                                                                                                                          ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAacac           <  4:135655/149‑1 (MQ=255)
                                                                                                                                           cATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACAcc          >  2:258488/1‑149 (MQ=255)
                                                                                                                                           cATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACAcc          >  1:37478/1‑149 (MQ=255)
                                                                                                                                            aTTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACAccc         >  2:309503/1‑149 (MQ=255)
                                                                                                                                             ttAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCa        >  3:243243/1‑149 (MQ=255)
                                                                                                                                              tAAATACGCACGCGGATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCt                <  1:602412/140‑1 (MQ=255)
                                                                                                                                              tAAATACGCACGCGGATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCt                >  4:330359/1‑140 (MQ=255)
                                                                                                                                              tAAATACGCACGCGGATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCt                <  3:330359/140‑1 (MQ=255)
                                                                                                                                                   ccGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGAc  <  2:408276/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTGCTAAATATCATTTCTGGTTGTGGCGTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAACGCCAGGAAATAACTCAATGGGCTGGTGGTGATATTATTGATGATTTCATCATAATGTAACCCATTGTCACCGCCTAACACCCAACAGAC  >  NC_000913/1546478‑1546773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: