Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,568,915 G→A 5.9% intergenic (‑92/+39) yddW ← / ← gadC liprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,568,9150GA 5.9% 337.6 / 8.2 103intergenic (‑92/+39)yddW/gadCliprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter
Reads supporting (aligned to +/- strand):  ref base G (49/47);  new base A (3/3);  total (52/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

Only 100 of 106 total aligned reads displayed.

AACTAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGC  >  NC_000913/1568767‑1569063
                                                                                                                                                    |                                                                                                                                                     
aaCTAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTGAATACGACAGCAAGCACGAAAAAGGGAGCGATg                                                                                                                                                       >  4:456897/1‑149 (MQ=255)
aaCTAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATg                                                                                                                                                       >  3:294803/1‑149 (MQ=255)
   tAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGa                                                                                                                                                      <  1:93748/147‑1 (MQ=255)
   tAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGa                                                                                                                                                      >  2:93748/1‑147 (MQ=255)
      tATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTAt                                                                                                                                                <  2:334043/149‑1 (MQ=255)
       aTCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATc                                                                                                                                               <  4:196300/149‑1 (MQ=255)
         cGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGc                                                                                                                                             <  2:284316/149‑1 (MQ=255)
           cTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCTc                                                                                                                                           <  2:187632/149‑1 (MQ=255)
            tGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCTcc                                                                                                                                          <  3:379832/149‑1 (MQ=255)
                   ctAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCt                                                                                                                                   >  1:431337/1‑149 (MQ=255)
                    tAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCTCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCtt                                                                                                                                  >  4:63547/1‑149 (MQ=255)
                    tAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCtt                                                                                                                                  >  4:155669/1‑149 (MQ=255)
                    tAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCtt                                                                                                                                  >  2:214088/1‑149 (MQ=255)
                    tAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCTc                                                                                                                                           <  3:286322/140‑1 (MQ=255)
                    tAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCTc                                                                                                                                           >  4:286322/1‑140 (MQ=255)
                       tCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTtata                                                                                                                               >  1:625454/1‑149 (MQ=255)
                         gTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAAc                                                                                                                             <  2:431337/149‑1 (MQ=255)
                         gTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAAc                                                                                                                             >  3:394695/1‑149 (MQ=255)
                          ttAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAAcc                                                                                                                            >  2:405844/1‑149 (MQ=255)
                          ttAATTTCTTGTATCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAAcc                                                                                                                            >  1:604648/1‑149 (MQ=255)
                              tttCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTc                                                                                                                        >  1:260487/1‑149 (MQ=255)
                                tCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCt                                                                                                                                   >  1:188465/1‑136 (MQ=255)
                                tCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCt                                                                                                                                   <  2:188465/136‑1 (MQ=255)
                                      ttCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATgg                                                                                                                <  1:128233/149‑1 (MQ=255)
                                      ttCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATgg                                                                                                                >  2:128233/1‑149 (MQ=255)
                                        cGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGtt                                                                                                              >  3:330626/1‑149 (MQ=255)
                                          ggAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAg                                                                                                            <  4:294803/149‑1 (MQ=255)
                                           gAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgt                                                                                                           <  2:625454/149‑1 (MQ=255)
                                             gCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgtgt                                                                                                         >  2:541555/1‑149 (MQ=255)
                                               aGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGttt                                                                                                       >  2:621861/1‑149 (MQ=255)
                                                gATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCCATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTc                                                                                                      <  4:411033/149‑1 (MQ=255)
                                                 atatCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCt                                                                                                     <  3:93158/149‑1 (MQ=255)
                                                  tatCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCtt                                                                                                    <  2:509085/149‑1 (MQ=255)
                                                   atCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTg                                                                                                   <  1:214088/149‑1 (MQ=255)
                                                    tCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGt                                                                                                  <  4:394695/149‑1 (MQ=255)
                                                    tCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGt                                                                                                  >  4:437833/1‑149 (MQ=255)
                                                    tCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGt                                                                                                  >  4:175821/1‑149 (MQ=255)
                                                    tCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGt                                                                                                  >  2:8292/1‑149 (MQ=255)
                                                      cATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtca                                                                                                >  4:2220/1‑149 (MQ=255)
                                                      cATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtca                                                                                                <  3:553863/149‑1 (MQ=255)
                                                      cATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtca                                                                                                <  3:170930/149‑1 (MQ=255)
                                                       atatGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTc                                                                                                                        >  2:407581/1‑124 (MQ=255)
                                                       atatGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTc                                                                                                                        <  1:407581/124‑1 (MQ=255)
                                                       atatGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTAGAACCATTCAGACATGGTTAGTGTTTCTTGtcat                                                                                               >  4:607283/1‑149 (MQ=255)
                                                         atGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtcattc                                                                                             >  3:32505/1‑149 (MQ=255)
                                                          tgtgCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtcattca                                                                                            <  4:330626/149‑1 (MQ=255)
                                                               tGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcaca                                                                                       >  1:300109/1‑149 (MQ=255)
                                                                    tCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAAtata                                                                                  >  1:84590/1‑149 (MQ=255)
                                                                     ccGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtcattc                                                                                             >  1:156449/1‑137 (MQ=255)
                                                                     ccGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtcattc                                                                                             <  2:156449/137‑1 (MQ=255)
                                                                      cGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgt                                                                                <  4:375541/149‑1 (MQ=255)
                                                                           aaCAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt                                                                           <  3:437833/149‑1 (MQ=255)
                                                                                 tgtgCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTCGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt                                                                     <  1:201405/149‑1 (MQ=255)
                                                                                 tgtgCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt                                                                     <  1:621861/149‑1 (MQ=255)
                                                                                   tgCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGc                                                                   >  2:38137/1‑149 (MQ=255)
                                                                                     cgcTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAc                                                                 >  3:558166/1‑149 (MQ=255)
                                                                                      gcTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcg                                                                >  3:672839/1‑149 (MQ=255)
                                                                                       cTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgtgt                                                                                                         <  4:527349/107‑1 (MQ=255)
                                                                                              tcGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCa                                                        >  4:5732/1‑149 (MQ=255)
                                                                                              tcGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCa                                                        >  3:12937/1‑149 (MQ=255)
                                                                                                gTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtg                                                                               >  2:115183/1‑124 (MQ=255)
                                                                                                gTACTTATCCCCGTTAAGTAAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtg                                                                               <  1:115183/124‑1 (MQ=255)
                                                                                                 tACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCa                                                                                                                       >  1:644320/1‑83 (MQ=255)
                                                                                                 tACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCa                                                                                                                       <  2:644320/83‑1 (MQ=255)
                                                                                                     tATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaagaa                                                 <  3:5732/149‑1 (MQ=255)
                                                                                                       tCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGt                                               <  3:456897/149‑1 (MQ=255)
                                                                                                        ccccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTg                                              >  3:250467/1‑149 (MQ=255)
                                                                                                         cccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGa                                             <  3:155669/149‑1 (MQ=255)
                                                                                                            gTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt                                                                           >  3:34088/1‑116 (MQ=255)
                                                                                                            gTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt                                                                           <  4:34088/116‑1 (MQ=255)
                                                                                                            gTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCt                                          >  3:466841/1‑149 (MQ=255)
                                                                                                               aaGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt                                       <  1:38137/149‑1 (MQ=255)
                                                                                                                 gTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTgg                                     >  3:309618/1‑149 (MQ=255)
                                                                                                                  tCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATGGTTCCCTGGTCTTATAGCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACTTGCGCGCGGGTGCAGGAAGCAGTGACCTTTTAGt                                    >  4:426521/1‑149 (MQ=255)
                                                                                                                    aaTACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGc                                  >  1:300868/1‑149 (MQ=255)
                                                                                                                    aaTACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGc                                  <  4:466841/149‑1 (MQ=255)
                                                                                                                     aTACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCg                                 <  4:32505/149‑1 (MQ=255)
                                                                                                                      tACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGt                                >  1:356520/1‑149 (MQ=255)
                                                                                                                       aCGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGtt                               <  3:2220/149‑1 (MQ=255)
                                                                                                                         gACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCt                             >  3:371380/1‑149 (MQ=255)
                                                                                                                         gACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCt                             <  4:12937/149‑1 (MQ=255)
                                                                                                                           cAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGa                           <  4:672839/149‑1 (MQ=255)
                                                                                                                                agcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGtt                      <  1:94325/149‑1 (MQ=255)
                                                                                                                                  caCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGa                    <  1:541555/149‑1 (MQ=255)
                                                                                                                                   aCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGAt                   <  4:371380/149‑1 (MQ=255)
                                                                                                                                   aCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGAt                   <  1:8292/149‑1 (MQ=255)
                                                                                                                                    cGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGAtt                  <  3:691184/149‑1 (MQ=255)
                                                                                                                                      gacaaGGGAGCGATAATTCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTgg                >  4:188792/4‑149 (MQ=255)
                                                                                                                                      aaaaaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTgg                >  1:81949/1‑149 (MQ=255)
                                                                                                                                      aaaaaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTgg                <  3:63547/149‑1 (MQ=255)
                                                                                                                                         aaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTc             <  2:604648/149‑1 (MQ=255)
                                                                                                                                           gggAGCGATAATTCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCa           >  4:170853/1‑149 (MQ=255)
                                                                                                                                            ggAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCag          >  1:383014/1‑149 (MQ=255)
                                                                                                                                             gAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCaga         >  1:633239/1‑149 (MQ=255)
                                                                                                                                             gAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCaga         >  1:540166/1‑149 (MQ=255)
                                                                                                                                                 gATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGa     >  2:179125/1‑149 (MQ=255)
                                                                                                                                                 gATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGa     <  2:300868/149‑1 (MQ=255)
                                                                                                                                                  aTGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGAc    >  2:427967/1‑149 (MQ=255)
                                                                                                                                                  aTGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTCGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGAc    >  1:495593/1‑149 (MQ=255)
                                                                                                                                                    gAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGc  <  4:309618/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACTAGTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGC  >  NC_000913/1568767‑1569063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: